Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,544,300	G→A	F108F (TTC→TTT)	ucpA ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,544,300	0	G	A	100.0%	131.7 / NA	38	F108F (TTC→TTT)	ucpA	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/18);  total (20/18)

CGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGT  >  NC_000913/2544165‑2544430                                                                                                                                        |                                                                                                                                   cGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAAt                                                                                                                                <  2:59964/140‑1 (MQ=255)  gTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATc                                                                                                                               <  2:267544/140‑1 (MQ=255)             aTCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCAt                                                                                                                    <  1:216131/140‑1 (MQ=255)             aTCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCAt                                                                                                                    <  2:601615/140‑1 (MQ=255)                 gCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCt                                                                                                                <  1:195904/140‑1 (MQ=255)                      caTATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCatat                                                                                                           >  2:489927/1‑140 (MQ=255)                              cAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGaaa                                                                                                   <  1:121100/140‑1 (MQ=255)                                gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACt                                                                                                 >  1:266108/1‑140 (MQ=255)                                gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACt                                                                                                 >  1:45381/1‑140 (MQ=255)                                 tGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTg                                                                                                >  2:401743/1‑140 (MQ=255)                                  gACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGc                                                                                               >  2:124635/1‑140 (MQ=255)                                   aCTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGcc                                                                                              <  1:401743/140‑1 (MQ=255)                                     tGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCa                                                                                            >  1:583024/1‑140 (MQ=255)                                       gggcagtcatcgCAATGCGGCCATCTGTGCGGGCAATCAGCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAga                                                                                          <  2:45835/134‑1 (MQ=255)                                           catcatCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAgacgac                                                                                      <  1:489927/140‑1 (MQ=255)                                                 cacaATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGc                                                                                <  1:754410/140‑1 (MQ=255)                                                      tGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCg                                                                           >  1:677019/1‑140 (MQ=255)                                                        cGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGccctgccc                                                                         >  1:45835/1‑134 (MQ=255)                                                         gACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTa                                                                        <  2:583024/140‑1 (MQ=255)                                                         gACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTa                                                                        >  2:97589/1‑140 (MQ=255)                                                          aCCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTAt                                                                       >  1:354168/1‑140 (MQ=255)                                                          aCCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTAt                                                                       >  2:345572/1‑140 (MQ=255)                                                           ccATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTAtt                                                                      <  1:97589/140‑1 (MQ=255)                                                              tCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAACCTGCCCAGACGACAAACGCCTg                                                                             >  2:763690/1‑130 (MQ=255)                                                              tCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTg                                                                             <  1:763690/130‑1 (MQ=255)                                                              tCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCAc                                                                   <  1:345572/140‑1 (MQ=255)                                                                tttGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCa                                                                 >  2:183916/1‑140 (MQ=255)                                                                        cAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCa                                                         >  1:823454/1‑140 (MQ=255)                                                                                     cagcaCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCcttctt                                            >  2:524373/1‑140 (MQ=255)                                                                                     cagcaCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCcttctt                                            >  2:834144/1‑140 (MQ=255)                                                                                             ccTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTc                                    <  2:266108/140‑1 (MQ=255)                                                                                              cTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcg                                   <  1:183916/140‑1 (MQ=255)                                                                                                                gCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                 >  2:395637/1‑124 (MQ=255)                                                                                                                gCCTTTAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                 <  1:395637/124‑1 (MQ=255)                                                                                                                    ttAATATTGATGTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTAc             >  1:803275/1‑140 (MQ=255)                                                                                                                               gTCAATATGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGt  >  1:524457/1‑140 (MQ=255)                                                                                                                                    tatGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCg     >  1:747783/1‑132 (MQ=255)                                                                                                                                    tatGAAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCg     <  2:747783/132‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGT  >  NC_000913/2544165‑2544430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑