Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,205	G→A	A34V (GCC→GTC)	eutT ←	putative ethanolamine utilization cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,205	0	G	A	100.0%	101.2 / NA	30	A34V (GCC→GTC)	eutT	putative ethanolamine utilization cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/14);  total (16/14)

CACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGA  >  NC_000913/2574070‑2574333                                                                                                                                        |                                                                                                                                 caCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACaga                                                                                                                              >  2:211054/1‑140 (MQ=255)  aCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACagag                                                                                                                             >  1:572305/1‑140 (MQ=255)    gCCTGCGGATGTTCATCGCTACTGGTAAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAggg                                                                                                                           >  2:375015/1‑140 (MQ=255)              gTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 <  1:211054/140‑1 (MQ=255)               ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGAc                                                                                                                <  1:675945/140‑1 (MQ=255)                         cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 >  1:167458/1‑129 (MQ=255)                         cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 <  2:167458/129‑1 (MQ=255)                          tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCa                                                                                                        <  2:561176/137‑1 (MQ=255)                          tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCa                                                                                                        >  1:561176/1‑137 (MQ=255)                             tCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGgaga                                                                                                  <  1:92054/140‑1 (MQ=255)                                 cccATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                                                                                              >  1:660764/1‑140 (MQ=255)                                 cccATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                                                                                              >  1:757537/1‑140 (MQ=255)                                      gAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGACCGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGCGAGATGGATctct                                                                                         >  2:785961/1‑140 (MQ=255)                                         cGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAg                                                                                                       <  1:413488/123‑1 (MQ=255)                                         cGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAg                                                                                                       >  2:413488/1‑123 (MQ=255)                                           ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  1:726545/1‑140 (MQ=255)                                           ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:75274/1‑140 (MQ=255)                                           ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:744068/1‑140 (MQ=255)                                           ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:686545/1‑140 (MQ=255)                                               gcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCg                                                                                <  1:809010/140‑1 (MQ=255)                                                cggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc                                                                               <  1:243812/140‑1 (MQ=255)                                                            ttCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGt                                                                   <  2:572305/140‑1 (MQ=255)                                                             tCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt                                                                  <  1:75274/140‑1 (MQ=255)                                                             tCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt                                                                  <  2:59682/140‑1 (MQ=255)                                                              cATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTc                                                                 <  2:660764/140‑1 (MQ=255)                                                                                           tCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATc                                    <  2:757537/140‑1 (MQ=255)                                                                                                      gATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGa                         >  2:706238/1‑140 (MQ=255)                                                                                                                         tCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAg      >  1:167843/1‑140 (MQ=255)                                                                                                                           cAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGc    >  2:475884/1‑140 (MQ=255)                                                                                                                             gCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGa  <  2:726545/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 CACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGA  >  NC_000913/2574070‑2574333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑