Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,633,034	T→C	D346G (GAC→GGC)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,633,034	0	T	C	100.0%	122.1 / NA	34	D346G (GAC→GGC)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/18);  total (16/18)

ACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGC  >  NC_000913/2632913‑2633166                                                                                                                          |                                                                                                                                     aCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATAc                                                                                                                    >  1:793374/1‑140 (MQ=255) aCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATAc                                                                                                                    >  1:707464/1‑140 (MQ=255)   ggTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCg                                                                                                                  >  2:824618/1‑140 (MQ=255)           ttGTAAAAACGGCCCGGGTAGAGTCCGTTTTCCCCCGGAGATTCTTCAGTTCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGc                                                                                                          <  2:707464/140‑1 (MQ=255)               aaGAACGGCCCTGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATa                                                                                                      <  1:30936/140‑1 (MQ=255)                         tgggTAGAGTTCGTTTTCCCCGGGAGTTTTTTCAGTCCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATAc                                                                                            <  2:611582/138‑1 (MQ=255)                              agagTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTg                                                                                       <  1:677337/140‑1 (MQ=255)                                  ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGGACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  2:702351/1‑140 (MQ=255)                                  ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  2:673074/1‑140 (MQ=255)                                  ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  1:354111/1‑140 (MQ=255)                                    cgttttCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTcc                                                                                 <  1:178223/137‑1 (MQ=255)                                                gagaTTCTTCAGTACCCGCCAGCATGGGACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACt                                                                     <  1:702351/140‑1 (MQ=255)                                                gagaTTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACt                                                                     <  1:269151/140‑1 (MQ=255)                                                    ttcttcAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGt                                                                 >  1:747791/1‑140 (MQ=255)                                                            tACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACa                                                         <  1:824618/140‑1 (MQ=255)                                                             ccccGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAg                                                        <  1:166896/139‑1 (MQ=255)                                                                 gCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGt                                                    >  1:141347/1‑140 (MQ=255)                                                                       aTGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTg                                              <  2:793374/140‑1 (MQ=255)                                                                                     cACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGc                                <  1:620422/140‑1 (MQ=255)                                                                                     cACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGc                                >  2:184794/1‑140 (MQ=255)                                                                                       ccGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCa                              >  2:723626/1‑140 (MQ=255)                                                                                        cgcgCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAg                             >  2:388359/1‑140 (MQ=255)                                                                                         gcgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGt                            <  2:354111/140‑1 (MQ=255)                                                                                         gcgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGt                            <  1:184794/140‑1 (MQ=255)                                                                                          cgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTc                           >  2:29503/1‑140 (MQ=255)                                                                                               gcgcCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGAt                      >  2:150128/1‑140 (MQ=255)                                                                                                 gcCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATAc                    >  1:109667/1‑140 (MQ=255)                                                                                                   cAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGa                  <  2:310622/140‑1 (MQ=255)                                                                                                   cAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGa                  <  1:150128/140‑1 (MQ=255)                                                                                                    aGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAg                 <  1:723626/140‑1 (MQ=255)                                                                                                     gcggcgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGt                >  2:650503/1‑140 (MQ=255)                                                                                                         cgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTa            <  1:388359/140‑1 (MQ=255)                                                                                                         cgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTa            <  2:747791/140‑1 (MQ=255)                                                                                                               cTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATa      <  2:218366/140‑1 (MQ=255)                                                                                                                   ggCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGc  >  2:546384/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                     ACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGC  >  NC_000913/2632913‑2633166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑