Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,634,926	T→C	V232A (GTT→GCT)	xseA →	exodeoxyribonuclease VII subunit XseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,634,926	0	T	C	100.0%	81.4 / NA	24	V232A (GTT→GCT)	xseA	exodeoxyribonuclease VII subunit XseA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

TGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGC  >  NC_000913/2634803‑2635054                                                                                                                            |                                                                                                                                 tGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCggg                                                                                                                  <  1:97189/140‑1 (MQ=255)   gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                >  2:846522/1‑140 (MQ=255)   gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                >  2:60066/1‑140 (MQ=255)        tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                           >  1:420346/1‑140 (MQ=255)        tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                           <  2:73644/140‑1 (MQ=255)                 tGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGa                                                                                                  >  2:346512/1‑140 (MQ=255)                                gATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttg                                                                                   <  1:346512/140‑1 (MQ=255)                                 aTCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgt                                                                                  >  1:731928/1‑140 (MQ=255)                                  tcgtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgtt                                                                                 <  1:60066/140‑1 (MQ=255)                                   cgtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgttg                                                                                <  2:836898/140‑1 (MQ=255)                                    gtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGc                                                                               >  2:548594/1‑140 (MQ=255)                                      cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                             >  1:651743/1‑140 (MQ=255)                                      cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTCAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                             >  1:333910/1‑140 (MQ=255)                                        ggCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGAt                                                                           >  1:753802/1‑140 (MQ=255)                                                                aTTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgcc                                                   <  1:73944/140‑1 (MQ=255)                                                                        aGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGa                                           >  1:399316/1‑140 (MQ=255)                                                                             tAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAagtag                                      >  1:167753/1‑140 (MQ=255)                                                                                      aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa                             <  2:280898/140‑1 (MQ=255)                                                                                      aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa                             <  1:548594/140‑1 (MQ=255)                                                                                            aGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGc                       <  2:731928/140‑1 (MQ=255)                                                                                                      aTTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACt             <  1:846522/140‑1 (MQ=255)                                                                                                        ttttACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTgc           >  2:793455/1‑140 (MQ=255)                                                                                                           tACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGcc        <  2:420346/140‑1 (MQ=255)                                                                                                                 ccGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGc  >  1:79323/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                 TGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGC  >  NC_000913/2634803‑2635054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑