| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,675,181 | G→A | A194V (GCG→GTG) | yphC ← | putative zinc‑binding dehydrogenase YphC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,675,181 | 0 | G | A | 97.7% | 142.6 / NA | 43 | A194V (GCG→GTG) | yphC | putative zinc‑binding dehydrogenase YphC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/23); minor base C (0/1); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTC > NC_000913/2675049‑2675311 | tCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGACGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGcat > 1:812640/1‑140 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCTGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 1:345039/140‑1 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 1:171982/140‑1 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 2:207211/140‑1 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 2:141201/140‑1 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 1:804126/140‑1 (MQ=255) aGACCTTCGGTGGTTGCTAAATAGACGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc < 2:812640/140‑1 (MQ=255) tCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGccc > 2:542784/1‑140 (MQ=255) gCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac > 1:10295/1‑140 (MQ=255) aaTAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGaccacc < 1:542784/140‑1 (MQ=255) cGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACg < 2:680970/140‑1 (MQ=255) ctCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGACCCCTTTTGCACCGCGACCTTTCCCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTAt < 2:177770/139‑1 (MQ=255) aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac > 2:61999/1‑126 (MQ=255) aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac < 1:61999/126‑1 (MQ=255) aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTAt > 1:388920/1‑140 (MQ=255) cATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCAc > 1:786083/1‑140 (MQ=255) aTCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACt < 1:408617/140‑1 (MQ=255) aTCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACt > 2:408617/1‑140 (MQ=255) tCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTg > 1:209448/1‑140 (MQ=255) aaCTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATGATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAAc > 2:138074/1‑140 (MQ=255) aaCTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAAc > 2:310609/1‑140 (MQ=255) tttGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTcgcc > 2:20612/1‑140 (MQ=255) gCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCcgcg < 1:778863/140‑1 (MQ=255) cATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCa < 1:20612/140‑1 (MQ=255) tCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCaaa > 2:439727/1‑140 (MQ=255) cGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCaaaa < 2:10295/140‑1 (MQ=255) tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGTCCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa < 2:786083/140‑1 (MQ=255) tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa < 2:209448/140‑1 (MQ=255) tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa < 2:319719/140‑1 (MQ=255) atatCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTAcc < 2:70024/140‑1 (MQ=255) tatCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCa > 2:360791/1‑140 (MQ=255) cGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAAcgccg < 2:291187/140‑1 (MQ=255) ccGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCa > 2:150060/1‑140 (MQ=255) tCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATa < 1:310609/140‑1 (MQ=255) cGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATaaa > 2:132709/1‑140 (MQ=255) gCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCAcc < 2:388920/140‑1 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt > 2:303174/1‑138 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt > 2:370642/1‑138 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt < 1:211788/138‑1 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt > 2:211788/1‑138 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt < 1:370642/138‑1 (MQ=255) cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt < 1:303174/138‑1 (MQ=255) cgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTc > 1:352220/1‑140 (MQ=255) | TCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTC > NC_000913/2675049‑2675311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |