Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,198	T→C	T63T (ACA→ACG)	era ←	30S ribosomal subunit maturation GTPase Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,198	0	T	C	100.0%	112.0 / NA	31	T63T (ACA→ACG)	era	30S ribosomal subunit maturation GTPase Era
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

GCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGT  >  NC_000913/2703062‑2703334                                                                                                                                         |                                                                                                                                         gCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       <  1:323624/140‑1 (MQ=255)  cACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTAt                                                                                                                                      <  1:93447/140‑1 (MQ=255)   aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       >  1:587474/1‑138 (MQ=255)   aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       <  2:587474/138‑1 (MQ=255)   aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATc                                                                                                                                     >  1:496720/1‑140 (MQ=255)       tttCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACg                                                                                                                                 >  1:190964/1‑140 (MQ=255)          cgtcgtCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAg                                                                                                                              >  2:107422/1‑140 (MQ=255)           gtcgtcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGa                                                                                                                             <  2:190964/140‑1 (MQ=255)            tcgtcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGAt                                                                                                                            <  1:588088/140‑1 (MQ=255)               tcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGc                                                                                                                         >  1:643196/1‑140 (MQ=255)                           cGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  1:360700/140‑1 (MQ=255)                           cGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  1:107422/140‑1 (MQ=255)                                  cTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTa                                                                                                      <  2:163942/140‑1 (MQ=255)                                  cTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTACTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTa                                                                                                      <  2:40097/140‑1 (MQ=255)                                           cAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             >  1:225238/1‑124 (MQ=255)                                           cAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  2:225238/124‑1 (MQ=255)                                                                    aaTAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTgcg                                                                    <  2:643196/140‑1 (MQ=255)                                                                      tAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGcc                                                                  <  2:496720/140‑1 (MQ=255)                                                                           ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                             >  2:594648/1‑140 (MQ=255)                                                                                             atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAtttt                                           >  1:82585/1‑140 (MQ=255)                                                                                                aGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTg                                        >  1:390161/1‑140 (MQ=255)                                                                                                        aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                <  2:390161/140‑1 (MQ=255)                                                                                                        aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                <  1:594648/140‑1 (MQ=255)                                                                                                            gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  2:194571/1‑140 (MQ=255)                                                                                                            gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  1:763670/1‑140 (MQ=255)                                                                                                            gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  2:690043/1‑140 (MQ=255)                                                                                                                gTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTc                        >  1:223356/1‑140 (MQ=255)                                                                                                                                cAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGc        <  2:763670/140‑1 (MQ=255)                                                                                                                                   gCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa     >  1:233883/1‑140 (MQ=255)                                                                                                                                   gCCCGGCGTATCGACGTAGATCACCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa     >  2:232691/1‑140 (MQ=255)                                                                                                                                      cGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGt  >  1:575359/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGT  >  NC_000913/2703062‑2703334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑