Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	305,808	T→C	Q337R (CAG→CGG)	ecpD ←	fimbrial adhesin EcpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	305,808	0	T	C	97.7%	309.1 / ‑2.4	87	Q337R (CAG→CGG)	ecpD	fimbrial adhesin EcpD
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (43/42);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GAAATCACGGGGTTTGATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCTGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGC  >  NC_000913/305672‑305941                                                                                                                                         |                                                                                                                                      gAAATCACGGGGTTTGATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCt                                                                                                                                    >  1:533566/1‑140 (MQ=255)    aTCACGGGGTTTGATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTaa                                                                                                                                 >  1:849122/1‑140 (MQ=255)       aCGGGGTTTGATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAaaga                                                                                                                              >  1:341672/1‑140 (MQ=255)                gATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCg                                                                                                                     >  1:204306/1‑140 (MQ=255)                    aTCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaa                                                                                                                 <  2:760317/140‑1 (MQ=255)                     tCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                >  1:642147/1‑140 (MQ=255)                     tCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                >  2:483256/1‑140 (MQ=255)                     tCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                <  2:259434/140‑1 (MQ=255)                     tCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                <  1:602230/140‑1 (MQ=255)                      cAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaaga                                                                                                               <  1:769696/140‑1 (MQ=255)                       aGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAATGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                >  2:470435/1‑138 (MQ=255)                       aGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAATGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGagaag                                                                                                                <  1:470435/138‑1 (MQ=255)                       aGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAgaagaa                                                                                                              <  2:204306/140‑1 (MQ=255)                       aGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAgaagaa                                                                                                              >  2:105458/1‑140 (MQ=255)                       aGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAgaagaa                                                                                                              >  2:477538/1‑140 (MQ=255)                        gCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGaaa                                                                                                             >  2:506361/1‑140 (MQ=255)                         cGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGaaaa                                                                                                            >  1:840317/1‑140 (MQ=255)                         cGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGaaaa                                                                                                            <  1:105458/140‑1 (MQ=255)                          gtgtTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTc                                                                                                                      >  2:737296/1‑129 (MQ=255)                          gtgtTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTc                                                                                                                      <  1:737296/129‑1 (MQ=255)                           tgtTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACa                                                                                                          <  1:483256/140‑1 (MQ=255)                           tgtTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACa                                                                                                          <  2:840317/140‑1 (MQ=255)                            gtTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACAt                                                                                                         >  1:538529/1‑140 (MQ=255)                                gAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGAt                                                                                                     <  1:506361/140‑1 (MQ=255)                                gAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGAt                                                                                                     <  1:455498/140‑1 (MQ=255)                                     aGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGt                                                                                                >  1:36357/1‑140 (MQ=255)                                     aGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCACTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGt                                                                                                <  2:285682/140‑1 (MQ=255)                                      gAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTc                                                                                               >  2:166174/1‑140 (MQ=255)                                       aaaaTTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCt                                                                                              >  1:673233/1‑140 (MQ=255)                                       aaaaTTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCt                                                                                              >  1:299022/1‑140 (MQ=255)                                              aTACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGctct                                                                                       >  1:794204/1‑140 (MQ=255)                                                aCCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTc                                                                                     <  1:454011/140‑1 (MQ=255)                                                  cagccagACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCAt                                                                                   <  2:319159/140‑1 (MQ=255)                                                    gccagACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCAtct                                                                                 >  2:70888/1‑140 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  1:51289/140‑1 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  2:36357/140‑1 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  2:341672/140‑1 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  1:70888/140‑1 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  2:82577/140‑1 (MQ=255)                                                          cTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCACTGATACCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  2:800823/140‑1 (MQ=255)                                                           tCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGaa                                                                          <  2:538529/140‑1 (MQ=255)                                                            ccGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAg                                                                         <  2:614805/140‑1 (MQ=255)                                                             cGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAgg                                                                        <  1:56444/140‑1 (MQ=255)                                                             cGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAgg                                                                        >  2:463647/1‑140 (MQ=255)                                                             cGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAgg                                                                        <  2:642147/140‑1 (MQ=255)                                                                   tgtgGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCTGCTTAAAGGAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAg                                                                         <  2:120541/133‑1 (MQ=255)                                                                   tgtgGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCTGCTTAAAGGAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAg                                                                         >  1:120541/1‑133 (MQ=255)                                                                       gtgtTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATa                                                              >  2:134182/1‑140 (MQ=255)                                                                         gtTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           >  2:95830/1‑125 (MQ=255)                                                                         gtTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGa                                                                           <  1:95830/125‑1 (MQ=255)                                                                         gtTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAgg                                                            <  2:794204/140‑1 (MQ=255)                                                                          ttCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGgtg                                                                      <  2:669151/129‑1 (MQ=255)                                                                          ttCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGgtg                                                                      >  1:669151/1‑129 (MQ=255)                                                                             tGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGt                                                        >  2:66275/1‑140 (MQ=255)                                                                              gAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTa                                                       <  2:123505/140‑1 (MQ=255)                                                                               aaaGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTAt                                                      >  1:497033/1‑140 (MQ=255)                                                                                  gCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGc                                                   >  1:460061/1‑140 (MQ=255)                                                                                      aaattGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCAc                                               >  1:686921/4‑140 (MQ=255)                                                                                               aGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCa                                      <  2:686921/140‑1 (MQ=255)                                                                                                 aTCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATg                                    <  2:108789/140‑1 (MQ=255)                                                                                                 aTCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATg                                    <  2:299022/140‑1 (MQ=255)                                                                                                 aTCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATg                                    <  2:747909/140‑1 (MQ=255)                                                                                                   cTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGaa                                  >  2:316078/1‑140 (MQ=255)                                                                                                      tGGTGTTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTg                               >  1:827352/1‑140 (MQ=255)                                                                                                         tgtTGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCa                            >  1:476230/1‑140 (MQ=255)                                                                                                           ttGATATCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCAtt                          >  2:846486/1‑140 (MQ=255)                                                                                                              atatCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAg                       >  2:414509/1‑140 (MQ=255)                                                                                                               tatCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGa                      <  1:66275/140‑1 (MQ=255)                                                                                                               tatCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGa                      <  2:673233/140‑1 (MQ=255)                                                                                                                atCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGAc                     >  1:832776/1‑140 (MQ=255)                                                                                                                 tCGCTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACt                    <  2:827352/140‑1 (MQ=255)                                                                                                                    cTGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGaa                 <  2:128700/140‑1 (MQ=255)                                                                                                                     tGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACt                                >  2:22264/1‑124 (MQ=255)                                                                                                                     tGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACt                                <  1:22264/124‑1 (MQ=255)                                                                                                                     tGATCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAAc                >  1:628440/1‑140 (MQ=255)                                                                                                                        tCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAACCATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGc             <  2:567837/140‑1 (MQ=255)                                                                                                                        tCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGc             >  1:567837/1‑140 (MQ=255)                                                                                                                        tCCCGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGc             >  2:432919/1‑140 (MQ=255)                                                                                                                         cccGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACt                    >  1:595811/1‑132 (MQ=255)                                                                                                                         cccGAGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCa            >  1:272892/1‑140 (MQ=255)                                                                                                                         cccGAGGTTCACCATCCGCTTAAAGAACTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACt                    <  2:595811/132‑1 (MQ=255)                                                                                                                             aGGTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATc        >  1:251596/1‑140 (MQ=255)                                                                                                                               gTTCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGt      >  1:857918/1‑140 (MQ=255)                                                                                                                                ttCACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTa     <  1:316078/140‑1 (MQ=255)                                                                                                                                  cACCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGCGTAGTAATAGGCGGAATTGCTTACCGGTTTCCATGAACTGCCATTCGGACTGCGATGCATATCGTAcc   >  2:68828/1‑139 (MQ=255)                                                                                                                                   aCCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGc  <  2:53306/140‑1 (MQ=255)                                                                                                                                   aCCATCCGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGc  >  1:731223/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GAAATCACGGGGTTTGATAATCAGCGTGTTGGAGGTAGAAAATTCATACCAGCCAGACTCCGGTGATGTGGTGTTCTGAAAGCGAAAGTTGAATAGATCTTTGGTGTTGATATCGCTGATCCCGAGGTTCACCATCTGCTTAAAGAAGTTGCTCGAGAAGAAAACATAGATCGAGTCTGCGCTCTTCATCTCGTTGAAGGTGTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGC  >  NC_000913/305672‑305941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑