Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,973	T→A	I348F (ATC→TTC)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,973	0	T	A	100.0%	97.3 / NA	29	I348F (ATC→TTC)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/14);  total (15/14)

TCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAA  >  NC_000913/2974838‑2975091                                                                                                                                        |                                                                                                                       tCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCAt                                                                                                                    <  2:13542/140‑1 (MQ=255) tCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCAt                                                                                                                    <  1:830933/140‑1 (MQ=255)        aaTGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGt                                                                                                             >  1:312566/1‑140 (MQ=255)        aaTGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGt                                                                                                             <  1:633091/140‑1 (MQ=255)           gCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCgg                                                                                                          >  1:641881/1‑140 (MQ=255)                                 ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                                                                    <  1:51034/140‑1 (MQ=255)                                 ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                                                                    <  2:641881/140‑1 (MQ=255)                                  cTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaaa                                                                                   >  1:636104/1‑140 (MQ=255)                                       ggATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTc                                                                              <  2:280157/140‑1 (MQ=255)                                              cTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAg                                                                       >  2:714803/1‑140 (MQ=255)                                                    aaCCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAg                                                                 <  2:42736/140‑1 (MQ=255)                                                         gAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAgcgccc                                                            <  2:636104/140‑3 (MQ=255)                                                              aaaaaGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAg                                                       >  2:417797/1‑140 (MQ=255)                                                                            ccTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGc                                         <  2:451377/140‑1 (MQ=255)                                                                             ctctTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCa                                        <  1:417797/140‑1 (MQ=255)                                                                              tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAg                                       >  1:53384/1‑140 (MQ=255)                                                                                  cttcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGc                                   <  1:771075/140‑1 (MQ=255)                                                                                                      tgtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgag               >  1:360542/1‑140 (MQ=255)                                                                                                       gtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgaga              >  2:71057/1‑140 (MQ=255)                                                                                                       gtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgaga              >  1:224737/1‑140 (MQ=255)                                                                                                         gCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGaaa            >  2:266988/1‑140 (MQ=255)                                                                                                         gCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGaaa            <  2:360542/140‑1 (MQ=255)                                                                                                              aCGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGc       <  2:72885/140‑1 (MQ=255)                                                                                                              aCGCGGCATCAACAAATGAGCGCAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGc       <  2:388560/140‑1 (MQ=255)                                                                                                               cgcgGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      >  1:484607/1‑140 (MQ=255)                                                                                                                  ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGa   >  2:285976/1‑140 (MQ=255)                                                                                                                  ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGa   >  1:733401/1‑140 (MQ=255)                                                                                                                  ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGC‑TTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      >  2:126032/1‑136 (MQ=255)                                                                                                                  ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGC‑TTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      <  1:126032/136‑1 (MQ=255)                                                                                                                   gCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGaa  >  1:241689/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                       TCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAA  >  NC_000913/2974838‑2975091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑