Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,047,411	C→T	G544D (GGT→GAT)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,047,411	0	C	T	100.0%	148.2 / NA	42	G544D (GGT→GAT)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

CTTTCATGATAATGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAACCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAGCGCCAG  >  NC_000913/3047276‑3047544                                                                                                                                        |                                                                                                                                      cTTTCATGATAATGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTc                                                                                                                                   >  1:283125/1‑140 (MQ=255)           aaTGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTcc                                                                                                                         >  2:55029/1‑140 (MQ=255)            aTGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCcg                                                                                                                        >  1:210951/1‑140 (MQ=255)             tGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCcgg                                                                                                                       >  1:165484/1‑140 (MQ=255)                  aaTCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGc                                                                                                                  <  1:494232/140‑1 (MQ=255)                    tCGCCAGCAGGCCCGCGTATTCGTCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGTCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAg                                                                                                                >  1:445662/1‑140 (MQ=255)                    tCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAg                                                                                                                <  2:210951/140‑1 (MQ=255)                    tCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAg                                                                                                                <  2:54060/140‑1 (MQ=255)                      gCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGaa                                                                                                              <  1:372387/140‑1 (MQ=255)                           cagGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGaa                                                                                                              <  2:579886/135‑1 (MQ=255)                           cagGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGaa                                                                                                              >  1:579886/1‑135 (MQ=255)                            agGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTg                                                                                                        <  1:160675/140‑1 (MQ=255)                                ccGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGt                                                                                                    <  1:492124/140‑1 (MQ=255)                                        tCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCaaa                                                                                            >  1:435218/1‑140 (MQ=255)                                                tgCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGcc                                                                                    >  1:374049/1‑140 (MQ=255)                                                 gcgcCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCa                                                                                   <  2:165484/140‑1 (MQ=255)                                                  cgcCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAg                                                                                  >  2:371369/1‑140 (MQ=255)                                                  cgcCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAg                                                                                  <  1:192688/140‑1 (MQ=255)                                                      agagTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGa                                                                              <  2:298143/140‑1 (MQ=255)                                                            cGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTggg                                                                         <  2:819357/139‑1 (MQ=255)                                                            cGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTggg                                                                         >  1:819357/1‑139 (MQ=255)                                                                  cATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCAtct                                                                  >  1:126154/1‑140 (MQ=255)                                                                          cGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTcggcggcg                                                          <  2:283125/140‑1 (MQ=255)                                                                            gCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCggcggcggc                                                        >  1:646802/1‑140 (MQ=255)                                                                             cgtcgtAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGgcggcggcg                                                       >  1:383080/1‑140 (MQ=255)                                                                               tcgtAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGtt                                                     >  1:203500/1‑140 (MQ=255)                                                                                       ggTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCa                                             >  1:755790/1‑140 (MQ=255)                                                                                        gTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCAt                                            <  1:55029/140‑1 (MQ=255)                                                                                                  ccagccagTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGaa                                  <  2:499591/140‑1 (MQ=255)                                                                                                     gccagTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAAccc                               >  2:708150/1‑140 (MQ=255)                                                                                                       cagTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAg                             >  2:703508/1‑140 (MQ=255)                                                                                                         gTCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCg                           <  2:755790/140‑1 (MQ=255)                                                                                                          tCAGCCAGCTGCGCAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCgg                          >  1:653459/1‑140 (MQ=255)                                                                                                                    gcgcAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGcc                >  1:608190/1‑140 (MQ=255)                                                                                                                       cAATCATCTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGa             >  2:744552/1‑140 (MQ=255)                                                                                                                         atcatcTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGAtt           <  2:126154/140‑1 (MQ=255)                                                                                                                          tcatcTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTc          <  1:703508/140‑1 (MQ=255)                                                                                                                            atcTGCTGATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAg        >  2:641891/1‑140 (MQ=255)                                                                                                                               tgctgATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAgcgc     <  2:646802/140‑1 (MQ=255)                                                                                                                                gctgATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAGCGcc    <  2:445662/140‑1 (MQ=255)                                                                                                                                 ctgATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAGCGCCa   <  2:653459/140‑1 (MQ=255)                                                                                                                                  tgATAATCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAGCGCCAg  >  2:779390/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CTTTCATGATAATGACGAATCGCCAGCAGGCCCGCGTATTCGCCCTGTGCGCCAGAGTTCGGCTGCATACAAACGGCGTCGTAACCGGTCAGTTTCACCAGCCAGTCAGCCAGCTGCGCAATCATCTGCTGATAACCTTCGGCCTGCTCCGGCGGGCAGAACGGGTGCAGTTCGGCAAATTCCGGCCAGGTGATTGGGATCATCTCGGCGGCGGCGTTCAGTTTCATGGTGCAGGAACCCAGCGGGATCATCGCCTGATTCAGCGCCAG  >  NC_000913/3047276‑3047544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑