Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,994	T→C	M1T (ATG→ACG) †	scpB →	methylmalonyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,994	0	T	C	100.0%	168.2 / NA	46	M1T (ATG→ACG) †	scpB	methylmalonyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

TTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAGCGATCTCA  >  NC_000913/3063857‑3064122                                                                                                                                          |                                                                                                                                 ttCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGt                                                                                                                                <  2:860523/140‑1 (MQ=255)      gAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTAt                                                                                                                           <  2:54975/140‑1 (MQ=255)        aTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCa                                                                                                                         >  1:491323/1‑140 (MQ=255)         tGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAg                                                                                                                        <  2:488696/140‑1 (MQ=255)               tttCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGtt                                                                                                                  <  2:726563/140‑1 (MQ=255)                ttCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTa                                                                                                                 <  2:855848/140‑1 (MQ=255)                 tCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTaa                                                                                                                <  2:297809/140‑1 (MQ=255)                    aTCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGt                                                                                                             >  1:265645/1‑140 (MQ=255)                        cTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTc                                                                                                         >  2:637823/1‑140 (MQ=255)                        cTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTc                                                                                                         <  2:548302/140‑1 (MQ=255)                                   aGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATcaacaa                                                                                              >  1:701712/1‑140 (MQ=255)                                   aGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATcaacaa                                                                                              >  2:695842/1‑140 (MQ=255)                                     aCGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAg                                                                                            >  2:628113/1‑140 (MQ=255)                                      cGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGt                                                                                           <  1:637823/140‑1 (MQ=255)                                                ggTCAAAAACAATACGCTCTCACCGCGCACCGACCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 <  2:388558/140‑1 (MQ=255)                                                  tCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 >  1:548436/1‑138 (MQ=255)                                                  tCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 <  2:548436/138‑1 (MQ=255)                                                   cAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAg                                                                              >  1:809567/1‑140 (MQ=255)                                                    aaaaaCAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGt                                                                             <  2:192577/140‑1 (MQ=255)                                                                  ctcACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               <  2:491323/140‑1 (MQ=255)                                                                   tcACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGaa                                                              <  1:245750/140‑1 (MQ=255)                                                                    cACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGaaa                                                             >  2:825159/1‑140 (MQ=255)                                                                        gcgcACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTTAATATAGCCCaaaaaat                                                         >  2:245750/1‑137 (MQ=255)                                                                        gcgcACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACtt                                                         >  2:190010/1‑140 (MQ=255)                                                                           cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               >  1:75224/1‑131 (MQ=255)                                                                           cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               <  2:75224/131‑1 (MQ=255)                                                                           cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTa                                                        <  2:172793/138‑1 (MQ=255)                                                                           cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTa                                                        >  1:172793/1‑138 (MQ=255)                                                                           cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAAt                                                      >  1:36487/1‑140 (MQ=255)                                                                              cGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGcc                                                   >  1:439805/1‑140 (MQ=255)                                                                               ggCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCt                                                  <  2:439805/140‑1 (MQ=255)                                                                                gCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCtt                                                 <  1:190010/140‑1 (MQ=255)                                                                                   tGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAg                                              >  2:497931/1‑140 (MQ=255)                                                                                       gCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTaaa                                          <  1:549116/140‑1 (MQ=255)                                                                                         aGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGt                                        <  1:825159/140‑1 (MQ=255)                                                                                           cTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCt                                      >  1:751039/1‑140 (MQ=255)                                                                                            tCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCtt                                     <  1:497931/140‑1 (MQ=255)                                                                                                tGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAAATATGGCCGAAAAATTAATGCCTTAAGTAAAAACTTTAtt                                 >  2:495677/1‑140 (MQ=255)                                                                                                  aaTTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATtga                               <  1:495677/140‑1 (MQ=255)                                                                                                  aaTTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATtga                               <  2:809567/140‑1 (MQ=255)                                                                                                     ttATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTTACTATGGCCGAAAACTTAATGCCTTAAGTAAAATCTTTATtgatga                            >  1:834643/1‑140 (MQ=255)                                                                                                                 aaTATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTCAAGTAAAGTCTTTATTGATGATCTTATGCAGGc                <  2:701712/140‑1 (MQ=255)                                                                                                                       ttGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAg          <  2:108127/140‑1 (MQ=255)                                                                                                                       ttGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAg          <  2:751039/140‑1 (MQ=255)                                                                                                                         gATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTTAGTTTTAATATGGCCGAAAAATTAATGACTTAAGTAAAGTCTTTATTGATGATCTTATTCAGGGGTTAAACg        >  1:204547/1‑140 (MQ=255)                                                                                                                               aGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGGGGTTAAAAATTGACCAAAAAATAAAGGCCTAAGATAAGGCTTTATTGATTATCTTATGCAAGAGTTCAGcactctaa  >  2:158926/1‑133 (MQ=255)                                                                                                                                          |                                                                                                                                 TTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAGCGATCTCA  >  NC_000913/3063857‑3064122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑