Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,103,886	C→T	H292Y (CAT→TAT)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,103,886	0	C	T	100.0%	91.3 / NA	28	H292Y (CAT→TAT)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

GCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCT  >  NC_000913/3103753‑3104010                                                                                                                                      |                                                                                                                             gcAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCt                                                                                                                        <  2:737382/140‑1 (MQ=255)     cGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAg                                                                                                                    >  2:865461/1‑140 (MQ=255)         cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCAt                                                                                                                >  2:804087/1‑140 (MQ=255)         cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCAt                                                                                                                <  1:648289/140‑1 (MQ=255)                   aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAg                                                                                                      <  2:230213/140‑1 (MQ=255)                     tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGat                                                                                                    <  1:114390/140‑1 (MQ=255)                          ggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATtgt                                                                                               <  1:865461/140‑1 (MQ=255)                          ggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATtgt                                                                                               >  2:684611/1‑140 (MQ=255)                               tttATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTa                                                                                          <  1:804087/140‑1 (MQ=255)                                        tttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTc                                                                                 >  2:303744/1‑140 (MQ=255)                                        tttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTc                                                                                 >  2:398416/1‑140 (MQ=255)                                         ttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTcc                                                                                >  2:17009/1‑140 (MQ=255)                                          tCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTccc                                                                               >  2:67158/1‑140 (MQ=255)                                             cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                            >  1:613292/1‑140 (MQ=255)                                             cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                            >  2:85652/1‑140 (MQ=255)                                                  tttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtcgtc                                                                       >  2:564940/1‑140 (MQ=255)                                                           gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                              <  1:684611/140‑1 (MQ=255)                                                                aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCa                                                         <  1:398416/140‑1 (MQ=255)                                                                aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCa                                                         <  2:613292/140‑1 (MQ=255)                                                                   tttGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATgg                                                      >  1:19585/1‑140 (MQ=255)                                                                     tGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAt                                                    <  1:85652/140‑1 (MQ=255)                                                                               gctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATg                                          <  1:303744/140‑1 (MQ=255)                                                                                  ggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcgc                                       <  1:564940/140‑1 (MQ=255)                                                                                    cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctc                                     <  2:347573/140‑1 (MQ=255)                                                                                    cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctc                                     <  1:67158/140‑1 (MQ=255)                                                                                         cGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGta                                >  2:287577/1‑140 (MQ=255)                                                                                         cGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGta                                <  1:17009/140‑1 (MQ=255)                                                                                                    tgcCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcgc                     >  1:335930/1‑140 (MQ=255)                                                                                                                       cTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCt  >  1:293284/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                             GCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCT  >  NC_000913/3103753‑3104010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑