Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,899	A→G	E329G (GAA→GGA)	kbaZ →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,899	0	A	G	100.0%	130.7 / NA	37	E329G (GAA→GGA)	kbaZ	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

TGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTG  >  NC_000913/3279768‑3280035                                                                                                                                    |                                                                                                                                         tGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGc                                                                                                                                  >  2:277941/1‑140 (MQ=255)  ggCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCa                                                                                                                                 <  2:598317/140‑1 (MQ=255)  ggCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCa                                                                                                                                 >  2:466705/1‑140 (MQ=255)      cAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGc                                                                                                                             <  1:636172/140‑1 (MQ=255)       aGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGcc                                                                                                                            <  2:534971/140‑1 (MQ=255)          gcTGACCTTCGCCCTGCGTTAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACt                                                                                                                         >  2:3937/1‑140 (MQ=255)              aCCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCa                                                                                                                     >  2:480586/1‑140 (MQ=255)                  tCGCCCTGCGTTAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGt                                                                                                                 <  1:3937/140‑1 (MQ=255)                          cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  2:160258/1‑140 (MQ=255)                          cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  1:721670/1‑140 (MQ=255)                          cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTAGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  2:285229/1‑140 (MQ=255)                               aGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcgc                                                                                                    <  1:391894/140‑1 (MQ=255)                                           tctGGCGGCGATTGAAGAAGAGCGGGTGCCAGCGAAAGCCTGCTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACAGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  2:593589/140‑1 (MQ=255)                                           tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  2:49526/140‑1 (MQ=255)                                           tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  1:496046/140‑1 (MQ=255)                                           tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        >  1:126115/1‑140 (MQ=255)                                            ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                                                                                       <  1:690142/140‑1 (MQ=255)                                            ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                                                                                       <  1:733566/140‑1 (MQ=255)                                               gcggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTATGGCGCGTgg                                                                                    >  1:593589/1‑140 (MQ=255)                                                cggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGt                                                                                   >  2:528106/1‑140 (MQ=255)                                                cggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGt                                                                                   >  2:169798/1‑140 (MQ=255)                                                                   ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGGAGACGTGATGCTCGACCGCCCGGGATACGGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:843060/140‑1 (MQ=255)                                                                   ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                >  1:674754/1‑140 (MQ=255)                                                                   ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:507700/140‑1 (MQ=255)                                                                   ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  1:480586/140‑1 (MQ=255)                                                                   ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:674754/140‑1 (MQ=255)                                                                       ccAGCGAAAGCCTGTTCTGGTTTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTgcg                                                            <  2:792772/140‑1 (MQ=255)                                                                       ccAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTgcg                                                            <  1:169798/140‑1 (MQ=255)                                                                                    gTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCg                                               <  2:721670/140‑1 (MQ=255)                                                                                      tCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGa                                             <  1:528106/140‑1 (MQ=255)                                                                                               cGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGAt                                    >  1:500280/1‑140 (MQ=255)                                                                                                                cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  2:132429/1‑140 (MQ=255)                                                                                                                cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  2:350272/1‑140 (MQ=255)                                                                                                                cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  1:705925/1‑140 (MQ=255)                                                                                                                cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   <  1:256921/140‑1 (MQ=255)                                                                                                                    aTGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCt               <  2:126115/140‑1 (MQ=255)                                                                                                                    aTGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCt               <  2:500280/140‑1 (MQ=255)                                                                                                                                 cGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTg  >  2:122909/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         TGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTG  >  NC_000913/3279768‑3280035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑