Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,293,977	T→C	V193A (GTG→GCG)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,293,977	0	T	C	100.0%	190.9 / NA	52	V193A (GTG→GCG)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

GGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACAATCCGGG  >  NC_000913/3293841‑3294109                                                                                                                                         |                                                                                                                                     gggCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGAt                                                                                                                                   >  2:298856/1‑140 (MQ=255) gggCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGAt                                                                                                                                   >  1:576407/1‑140 (MQ=255)      tCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACg                                                                                                                              >  2:354403/1‑140 (MQ=255)      tCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACg                                                                                                                              >  1:245536/1‑140 (MQ=255)      tCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACg                                                                                                                              >  1:310481/1‑140 (MQ=255)      tCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAAAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACg                                                                                                                              <  2:687884/140‑1 (MQ=255)           ccATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCcgac                                                                                                                         >  2:25763/1‑140 (MQ=255)                  tttACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAata                                                                                                                  <  2:181752/140‑1 (MQ=255)                   ttACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGTTCAACGCCGACGAAAatat                                                                                                                 >  2:353162/1‑140 (MQ=255)                   ttACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAatat                                                                                                                 <  1:633997/140‑1 (MQ=255)                            gcgTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAgg                                                                                                        <  1:25763/140‑1 (MQ=255)                             cgTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAggc                                                                                                       >  2:606202/1‑140 (MQ=255)                                    tGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGctggctgg                                                                                                <  2:494805/140‑1 (MQ=255)                                        cAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAgg                                                                                                        <  1:154328/128‑1 (MQ=255)                                        cAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAgg                                                                                                        >  2:154328/1‑128 (MQ=255)                                        cAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCt                                                                                            >  2:460295/1‑140 (MQ=255)                                          ggAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGc                                                                                          >  2:789670/1‑140 (MQ=255)                                          ggAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGc                                                                                          >  2:156497/1‑140 (MQ=255)                                                gctgctTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGcgcg                                                                                    >  1:380026/1‑140 (MQ=255)                                                gctgctTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGcgcg                                                                                    >  1:87434/1‑140 (MQ=255)                                                 ctgctTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGTTCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGt                                                                                   <  1:353162/140‑1 (MQ=255)                                                   gctTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCt                                                                                 <  2:380026/140‑1 (MQ=255)                                                   gctTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCt                                                                                 >  1:135610/1‑140 (MQ=255)                                                     ttGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTgg                                                                               >  1:221678/1‑140 (MQ=255)                                                         cgcgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTg                                                                           >  2:355354/1‑140 (MQ=255)                                                          gcgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGa                                                                          <  2:221678/140‑1 (MQ=255)                                                          gcgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGa                                                                          <  2:821311/140‑1 (MQ=255)                                                           cgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGAt                                                                         <  2:576407/140‑1 (MQ=255)                                                           cgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGAt                                                                         <  1:354403/140‑1 (MQ=255)                                                           cgAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGAt                                                                         <  1:298856/140‑1 (MQ=255)                                                                   aaaCAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTaa                                                                 <  2:87434/140‑1 (MQ=255)                                                                   aaaCAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTaa                                                                 <  1:789670/140‑1 (MQ=255)                                                                   aaaCAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTaa                                                                 <  1:156497/140‑1 (MQ=255)                                                                        gcagAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATc                                                            >  2:58836/1‑140 (MQ=255)                                                                                 tGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACatga                                                   >  2:143094/1‑140 (MQ=255)                                                                                  gATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACatgat                                                  <  1:460295/140‑1 (MQ=255)                                                                                        aCCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGc                                            >  1:520945/1‑140 (MQ=255)                                                                                         ccTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACatga                                                   <  2:97946/132‑1 (MQ=255)                                                                                         ccTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACatga                                                   >  1:97946/1‑132 (MQ=255)                                                                                                   gcTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCg                                 >  1:398556/1‑140 (MQ=255)                                                                                                    ctctCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGa                                <  1:143094/140‑1 (MQ=255)                                                                                                               gACTCAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAAc                     <  2:520945/140‑1 (MQ=255)                                                                                                                   cAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTa                 >  2:571952/1‑140 (MQ=255)                                                                                                                   cAGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTa                 >  1:803892/1‑140 (MQ=255)                                                                                                                    aGGAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTAt                <  2:803892/140‑1 (MQ=255)                                                                                                                      gAACAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATcc              >  2:747615/1‑140 (MQ=255)                                                                                                                         cAGGCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGaa           <  1:747615/140‑1 (MQ=255)                                                                                                                           ggCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACa         >  2:128044/1‑140 (MQ=255)                                                                                                                            gCGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACaa        >  1:688464/1‑140 (MQ=255)                                                                                                                             cGAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACAAt       <  2:310481/140‑1 (MQ=255)                                                                                                                              gAATACGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACAATc      >  2:786389/1‑140 (MQ=255)                                                                                                                                  aCGCTGGCGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACAATCCggg  <  1:355354/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAGGCTGGCTGGATCTGCAGCGCGTCTGGTTTGATAACCGTAACGATCCCGACATGATGAAAGCCGGGATCGCCGACTGGCAGAAACGTTATCCGAACAATCCGGG  >  NC_000913/3293841‑3294109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑