Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,229	T→C	D462G (GAT→GGT)	yhbX ←	putative hydrolase, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,229	0	T	C	100.0%	204.8 / NA	57	D462G (GAT→GGT)	yhbX	putative hydrolase, inner membrane
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

TCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCG  >  NC_000913/3320096‑3320364                                                                                                                                      |                                                                                                                                        tcCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCaa                                                                                                                                   >  1:147815/1‑140 (MQ=255)    tttATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGaa                                                                                                                                <  1:212487/140‑1 (MQ=255)     ttATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCGAGAAc                                                                                                                               <  2:162725/140‑1 (MQ=255)     ttATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAAc                                                                                                                               <  2:707695/140‑1 (MQ=255)           tgtgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCt                                                                                                                         >  2:232703/1‑140 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAAc                                                                                                                               <  1:466264/132‑1 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAAc                                                                                                                               >  1:64193/1‑132 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAAc                                                                                                                               >  2:466264/1‑132 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAAc                                                                                                                               <  2:64193/132‑1 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACg                                                                                                                              <  2:550770/133‑1 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACg                                                                                                                              >  1:550770/1‑133 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCtat                                                                                                                       >  2:649032/1‑140 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCtat                                                                                                                       >  2:203134/1‑140 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGGAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGCTGTGCGATCCACGCCACCCCCAAGAACGGGGCtat                                                                                                                       >  1:166194/1‑140 (MQ=255)             tgCAATCACTTCCTCAAGCGTTTGCGGCTGGTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCtat                                                                                                                       >  2:732679/1‑140 (MQ=255)                aaTCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATAcc                                                                                                                    >  1:375019/1‑140 (MQ=255)                     cTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATa                                                                                                               >  1:166714/1‑140 (MQ=255)                       tCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACgggg                                                                                                                           <  2:547021/126‑1 (MQ=255)                       tCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACgggg                                                                                                                           >  1:547021/1‑126 (MQ=255)                          tCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACAt                                                                                                          <  1:232703/140‑1 (MQ=255)                          tCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACAt                                                                                                          <  2:166714/140‑1 (MQ=255)                           cAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATc                                                                                                         >  1:808324/1‑140 (MQ=255)                            aaGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATAc                                                                                                                     >  1:660044/1‑127 (MQ=255)                            aaGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATAc                                                                                                                     <  2:660044/127‑1 (MQ=255)                               cGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGa                                                                                                     <  2:808324/140‑1 (MQ=255)                                gTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGAc                                                                                                    >  1:23240/1‑140 (MQ=255)                                            tCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCctg                                                                                        <  2:635820/140‑1 (MQ=255)                                                     gTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGc                                                                               <  1:203134/140‑1 (MQ=255)                                                           cccATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCt                                                                         >  1:52361/1‑140 (MQ=255)                                                              aTCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGcc                                                                      >  1:660590/1‑140 (MQ=255)                                                              aTCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGcc                                                                      >  2:57780/1‑140 (MQ=255)                                                                   cGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCAt                                                                 <  2:52361/140‑1 (MQ=255)                                                                   cGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGCTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCGCTGCCTCCAt                                                                 >  2:570119/1‑140 (MQ=255)                                                                          ataaGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATa                                                          >  1:699875/1‑140 (MQ=255)                                                                              ggTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACg                                                      <  1:732679/140‑1 (MQ=255)                                                                                     attGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttcttct                                               >  1:418696/1‑140 (MQ=255)                                                                                         tAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGt                                           >  2:476960/1‑140 (MQ=255)                                                                                         tAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGt                                           >  2:555148/1‑140 (MQ=255)                                                                                          aaGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTa                                          <  2:23240/140‑1 (MQ=255)                                                                                           aGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAg                                         >  1:742881/1‑140 (MQ=255)                                                                                             cTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAggg                                       <  2:107895/140‑1 (MQ=255)                                                                                             cTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAggg                                       >  1:491014/1‑140 (MQ=255)                                                                                              tGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttcttctt                                              >  2:502013/1‑132 (MQ=255)                                                                                              tGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttcttctt                                              <  1:502013/132‑1 (MQ=255)                                                                                               gTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTc                                     <  1:649032/140‑1 (MQ=255)                                                                                               gTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTc                                     <  1:555148/140‑1 (MQ=255)                                                                                                tCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTc                                     <  1:523722/139‑1 (MQ=255)                                                                                                tCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTc                                     >  2:523722/1‑139 (MQ=255)                                                                                                tCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCa                                    <  2:51250/140‑1 (MQ=255)                                                                                                tCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCa                                    >  2:83501/1‑140 (MQ=255)                                                                                                          gttgttTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCg                                            <  1:361122/122‑1 (MQ=255)                                                                                                          gttgttTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCg                                            >  2:361122/1‑122 (MQ=255)                                                                                                          gttgttTTCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGc                          >  1:500391/1‑140 (MQ=255)                                                                                                                 tCCGTTGTGCGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTc                   <  1:83501/140‑1 (MQ=255)                                                                                                                          cGATCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATa          <  1:57780/140‑1 (MQ=255)                                                                                                                             tCCACGCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACAt       >  1:326739/1‑140 (MQ=255)                                                                                                                                  gCCACCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCg  >  2:14577/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAGCTGTCGAAAAGATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCG  >  NC_000913/3320096‑3320364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑