Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,109	T→C	L127L (TTA→TTG)	nanR ←	DNA‑binding transcriptional dual regulator NanR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,109	0	T	C	100.0%	161.0 / NA	45	L127L (TTA→TTG)	nanR	DNA‑binding transcriptional dual regulator NanR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/26);  total (19/26)

CGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACC  >  NC_000913/3373973‑3374238                                                                                                                                         |                                                                                                                                  cGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAAt                                                                                                                                >  2:471362/1‑140 (MQ=255)       aaCGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCg                                                                                                                          >  1:697568/1‑140 (MQ=255)         cGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGaa                                                                                                                        <  1:522276/140‑1 (MQ=255)           aatgaatgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAAt                                                                                                                      <  1:309120/140‑1 (MQ=255)                 tgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCaa                                                                                                                <  2:397009/140‑1 (MQ=255)                 tgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCaa                                                                                                                >  1:682944/1‑140 (MQ=255)                      cgTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCa                                                                                                           >  2:524498/1‑140 (MQ=255)                       gttgttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCAc                                                                                                          <  2:131390/140‑1 (MQ=255)                               ccAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  <  2:596534/140‑1 (MQ=255)                                   cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  <  2:506618/136‑1 (MQ=255)                                   cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  >  1:506618/1‑136 (MQ=255)                                   cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGaaa                                                                                              >  1:133071/1‑140 (MQ=255)                                         actgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAAt                                                                                        >  1:673128/1‑140 (MQ=255)                                         actgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAAt                                                                                        >  2:524761/1‑140 (MQ=255)                                               gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  2:686114/140‑1 (MQ=255)                                               gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  1:524761/140‑1 (MQ=255)                                               gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  2:133071/140‑1 (MQ=255)                                                       gTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCg                                                                          >  1:6034/1‑140 (MQ=255)                                                         gCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  1:471362/140‑1 (MQ=255)                                                             ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        >  1:466219/1‑136 (MQ=255)                                                             ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  2:466219/136‑1 (MQ=255)                                                             ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGc                                                                    >  2:836341/1‑140 (MQ=255)                                                             ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGc                                                                    <  1:843354/140‑1 (MQ=255)                                                               gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        >  1:571445/1‑134 (MQ=255)                                                               gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  2:571445/134‑1 (MQ=255)                                                               gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTc                                                                  <  2:146250/140‑1 (MQ=255)                                                                 cAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCAc                                                                <  2:690127/140‑1 (MQ=255)                                                                 cAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCAc                                                                <  2:682944/140‑1 (MQ=255)                                                                    cAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCga                                                             >  2:493121/1‑140 (MQ=255)                                                                           aTTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCCCCAGACAGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAgt                                                      <  2:802703/140‑1 (MQ=255)                                                                           aTTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAgt                                                      <  2:391297/140‑1 (MQ=255)                                                                                cTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCg                                                 <  1:854272/140‑1 (MQ=255)                                                                                    tCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGa                                             <  1:379961/140‑1 (MQ=255)                                                                                      ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAg                                           <  1:836341/140‑1 (MQ=255)                                                                                      ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAg                                           <  2:697568/140‑1 (MQ=255)                                                                                                         tAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGcgcg                        >  2:32407/1‑140 (MQ=255)                                                                                                            cgcACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTc                     >  2:710985/1‑140 (MQ=255)                                                                                                               aCCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGcc                  >  1:708088/1‑140 (MQ=255)                                                                                                               aCCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGcc                  <  2:695553/140‑1 (MQ=255)                                                                                                                ccAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCg                 >  2:841328/1‑140 (MQ=255)                                                                                                                   gACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCgttg              <  2:6034/140‑1 (MQ=255)                                                                                                                   gACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCgttg              >  1:634919/1‑140 (MQ=255)                                                                                                                        gATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTAt         <  1:841328/140‑1 (MQ=255)                                                                                                                                   cAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCAcc  >  2:307922/1‑136 (MQ=255)                                                                                                                                   cAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCAcc  <  1:307922/136‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACC  >  NC_000913/3373973‑3374238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑