Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,101,728	(G)7→8	coding (39/984 nt)	kdgT →	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,721	1	.	G	100.0%	289.2 / NA	77	P11R (CCG→CGG)	kdgT	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (37/40);  total (37/40)

GATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTT  >  NC_000913/4101582‑4101855                                                                                                                                             |                                                                                                                                         gATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATccc                                                                                                                                           <  2:55546/140‑1 (MQ=255)    cACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑‑ggg                                                                                                                                     <  2:325235/140‑1 (MQ=255)       ttttATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑‑gggggg                                                                                                                                  >  2:1086027/1‑140 (MQ=255)                 tAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCt                                                                                                                         >  1:475240/1‑140 (MQ=255)                 tAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCt                                                                                                                         >  2:226466/1‑140 (MQ=255)                 tAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCt                                                                                                                         >  1:793904/1‑140 (MQ=255)                 tAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCt                                                                                                                         >  2:157042/1‑140 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       <  1:497687/140‑1 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       <  2:294047/140‑1 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       >  1:188515/1‑140 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       <  1:1477646/140‑1 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       <  1:1210370/140‑1 (MQ=255)                   aatgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCg                                                                                                                       >  1:1122981/1‑140 (MQ=255)                    atgaatgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGt                                                                                                                      <  2:1070303/140‑1 (MQ=255)                        atgTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCg                                                                                                                  <  1:1290521/140‑1 (MQ=255)                             tATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTAtt                                                                                                             >  2:625882/1‑140 (MQ=255)                             tATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGACAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTAtt                                                                                                             >  1:438132/1‑140 (MQ=255)                                  tCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTg                                                                                                        <  2:1450262/140‑1 (MQ=255)                                     tttCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGgcg                                                                                                     >  1:1619459/1‑140 (MQ=255)                                     tttCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGgcg                                                                                                     <  1:376164/140‑1 (MQ=255)                                     tttCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGgcg                                                                                                     <  1:322648/140‑1 (MQ=255)                                     tttCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGgcg                                                                                                     <  2:1555002/140‑1 (MQ=255)                                       tCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCa                                                                                                   <  2:1594861/140‑1 (MQ=255)                                       tCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCa                                                                                                   <  1:430459/140‑1 (MQ=255)                                        ccATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAc                                                                                                  <  1:1409468/140‑1 (MQ=255)                                        ccATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCCACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAAACCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAc                                                                                                  <  2:523714/140‑1 (MQ=255)                                         cATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCa                                                                                                   <  2:674075/140‑1 (MQ=255)                                                  tGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCacac                                                                                        >  1:1094297/1‑140 (MQ=255)                                                  tGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCacac                                                                                        >  1:1124307/1‑140 (MQ=255)                                                   gATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                                                       >  2:61957/1‑140 (MQ=255)                                                   gATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                                                       >  1:513837/1‑140 (MQ=255)                                                   gATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAAGGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                                                       <  1:1439832/140‑1 (MQ=255)                                                    aTGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCt                                                                                      >  1:36896/1‑140 (MQ=255)                                                    aTGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGAGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCt                                                                                      >  2:1139304/1‑140 (MQ=255)                                                       cTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTtct                                                                                   >  1:1049569/1‑140 (MQ=255)                                                       cTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTtct                                                                                   >  1:324593/1‑140 (MQ=255)                                                       cTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTtct                                                                                   >  2:366460/1‑140 (MQ=255)                                                        tCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTtctc                                                                                  >  1:482292/1‑140 (MQ=255)                                                         ccATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCg                                                                                 >  2:1630785/1‑140 (MQ=255)                                                         ccATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCg                                                                                 >  1:1536707/1‑140 (MQ=255)                                                              gAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggg                                                                            <  1:1167854/140‑1 (MQ=255)                                                                ggAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggggg                                                                          <  2:673819/140‑1 (MQ=255)                                                                    ttACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCggg                                                                      >  1:870567/1‑140 (MQ=255)                                                                    ttACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCggg                                                                      >  2:147173/1‑140 (MQ=255)                                                                     tACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCgggg                                                                     <  2:793904/140‑1 (MQ=255)                                                                            cAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtt                                                              >  1:510442/1‑140 (MQ=255)                                                                            cAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtt                                                              >  1:445159/1‑140 (MQ=255)                                                                            cAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtt                                                              >  1:1321295/1‑140 (MQ=255)                                                                            cAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtt                                                              >  1:1130306/1‑140 (MQ=255)                                                                              gCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtttt                                                            >  2:1394996/1‑140 (MQ=255)                                                                               cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                                                           <  2:99783/140‑1 (MQ=255)                                                                               cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                                                           <  1:1086027/140‑1 (MQ=255)                                                                               cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                                                           >  1:1102972/1‑140 (MQ=255)                                                                               cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                                                           <  1:273119/140‑1 (MQ=255)                                                                                 cTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGa                                                         <  2:1222925/140‑1 (MQ=255)                                                                                    aaaaaTATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCa                                                      >  2:1522207/1‑140 (MQ=255)                                                                                      aaaTATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCAtt                                                    <  1:466955/140‑1 (MQ=255)                                                                                         tatGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc                                                 >  1:225554/1‑140 (MQ=255)                                                                                            gCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCaa                                              >  1:1301284/1‑140 (MQ=255)                                                                                            gCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCaa                                              >  1:1301285/1‑140 (MQ=255)                                                                                             ccGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAAc                                             <  1:34217/140‑1 (MQ=255)                                                                                              cGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACg                                            <  1:625882/140‑1 (MQ=255)                                                                                              cGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACg                                            <  2:49090/140‑1 (MQ=255)                                                                                                   ggTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGgatg                                       <  2:1049569/140‑1 (MQ=255)                                                                                                     tGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGgatgat                                     <  2:733092/140‑1 (MQ=255)                                                                                                     tGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGgatgat                                     <  2:1619459/140‑1 (MQ=255)                                                                                                       aTGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTa                                   >  1:1061327/1‑140 (MQ=255)                                                                                                              tGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTAc                            >  2:341962/1‑140 (MQ=255)                                                                                                              tGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTAc                            <  2:36896/140‑1 (MQ=255)                                                                                                              tGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTAc                            >  2:505176/1‑140 (MQ=255)                                                                                                              tGCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTAc                            <  2:188515/140‑1 (MQ=255)                                                                                                               gCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACg                           <  1:226466/140‑1 (MQ=255)                                                                                                               gCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACg                           <  1:157042/140‑1 (MQ=255)                                                                                                               gCAGATAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACg                           <  2:1124307/140‑1 (MQ=255)                                                                                                                   aTAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGc                       >  1:660130/1‑140 (MQ=255)                                                                                                                    tAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGcc                      <  2:475240/140‑1 (MQ=255)                                                                                                                    tAAAACGCTCGATTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGcc                      <  1:600824/140‑1 (MQ=255)                                                                                                                               aTTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCgg           <  2:510442/140‑1 (MQ=255)                                                                                                                               aTTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCgg           <  2:1130306/140‑1 (MQ=255)                                                                                                                               aTTGAGAAAATCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCgg           <  2:1321295/140‑1 (MQ=255)                                                                                                                                       aaTCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGttt   <  2:1536707/140‑1 (MQ=255)                                                                                                                                        aTCCCG‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGtttt  <  2:1061327/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                         GATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTT  >  NC_000913/4101582‑4101855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑