Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,610,540 +C coding (377/546 nt) hyfH → hydrogenase 4 component H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,610,5361.C99.1% 400.9 / ‑5.0 108A125P (GCC→CCC) hyfHhydrogenase 4 component H
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (72/35);  minor base T (1/0);  total (73/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 119 total aligned reads displayed.

CGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑CCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGA  >  NC_000913/2610397‑2610669
                                                                                                                                            |                                                                                                                                     
cGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTg                                                                                                                                        >  2:1613720/1‑140 (MQ=255)
 ggACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑c                                                                                                                                      >  2:792640/1‑140 (MQ=255)
 ggACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑c                                                                                                                                      >  2:553967/1‑140 (MQ=255)
 ggACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑c                                                                                                                                      >  2:365249/1‑140 (MQ=255)
 ggACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTGCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑c                                                                                                                                      >  2:1306246/1‑140 (MQ=255)
 ggACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCCCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑c                                                                                                                                      >  2:161256/1‑140 (MQ=255)
    cGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑cccc                                                                                                                                   <  2:1145547/140‑1 (MQ=255)
       tgtgAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGGTGCAGCCGTTGCGAACGCCCGTTTG‑cccccgc                                                                                                                                >  2:559921/1‑137 (MQ=255)
         tgAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑cccccgcaa                                                                                                                              <  1:339405/140‑6 (MQ=255)
               agTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAAcc                                                                                                                         >  2:1379872/1‑140 (MQ=255)
                 tgtgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCAt                                                                                                                       >  1:1185077/1‑140 (MQ=255)
                 tgtgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCAt                                                                                                                       >  2:573157/1‑140 (MQ=255)
                 tgtgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCAt                                                                                                                       >  2:125523/1‑140 (MQ=255)
                   tgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCg                                                                                                                     >  2:1607816/1‑140 (MQ=255)
                   tgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCg                                                                                                                     >  1:599523/1‑140 (MQ=255)
                   tgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCg                                                                                                                     >  1:146903/1‑140 (MQ=255)
                   tgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCg                                                                                                                     >  1:1625628/1‑140 (MQ=255)
                   tgCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCg                                                                                                                     >  2:81611/1‑140 (MQ=255)
                         aCCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTgg                                                                                                               <  2:1314229/140‑1 (MQ=255)
                          ccAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGgc                                                                                                              >  1:1528383/1‑140 (MQ=255)
                             gagCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGgctgc                                                                                                           >  2:460083/1‑140 (MQ=255)
                             gagCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGgctgc                                                                                                           >  2:977069/1‑140 (MQ=255)
                             gagCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGgctgc                                                                                                           <  2:111186/140‑1 (MQ=255)
                                   tCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAAtt                                                                                                     >  1:1598134/1‑140 (MQ=255)
                                    ccAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTg                                                                                                    >  2:689616/1‑140 (MQ=255)
                                     cAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGt                                                                                                   <  1:546609/140‑1 (MQ=255)
                                        cTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAg                                                                                                >  1:1502201/1‑140 (MQ=255)
                                          tACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGca                                                                                              <  1:1007962/140‑1 (MQ=255)
                                           aCCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGcac                                                                                             >  2:545931/1‑140 (MQ=255)
                                             cAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAg                                                                                           >  2:1026882/1‑140 (MQ=255)
                                                     ttGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACaaaa                                                                                   >  2:1327406/1‑140 (MQ=255)
                                                     ttGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACaaaa                                                                                   <  1:1463810/140‑1 (MQ=255)
                                                      tGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAAt                                                                                  >  2:1019094/1‑140 (MQ=255)
                                                      tGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAAt                                                                                  >  1:1091936/1‑140 (MQ=255)
                                                       gAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATg                                                                                 <  1:229430/140‑1 (MQ=255)
                                                       gAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATg                                                                                 >  1:1112180/1‑140 (MQ=255)
                                                       gAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATg                                                                                 <  1:553967/140‑1 (MQ=255)
                                                       gAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATg                                                                                 <  2:1625628/140‑1 (MQ=255)
                                                       gAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATg                                                                                 <  1:1379872/140‑1 (MQ=255)
                                                             aCCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCcac                                                                           >  2:747561/1‑140 (MQ=255)
                                                              ccGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCACAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCcaca                                                                          >  2:1360928/1‑140 (MQ=255)
                                                                       aTAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTTAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGa                                                                 >  1:1375569/1‑140 (MQ=255)
                                                                           aGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAAtg                                                             >  1:961878/1‑140 (MQ=255)
                                                                             ccGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAAtgtt                                                           <  1:460083/140‑1 (MQ=255)
                                                                                aTCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTtgtg                                                        >  2:340802/1‑140 (MQ=255)
                                                                                           cgcgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcga                                             >  1:895775/1‑140 (MQ=255)
                                                                                            gcgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCACAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgag                                            <  1:1360928/140‑1 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGTCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:112265/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  2:891020/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:641089/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:1360711/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:922254/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  2:935699/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:989618/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  1:548901/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  2:1065405/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  2:1648151/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagc                                           >  2:1204806/1‑140 (MQ=255)
                                                                                             cgcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCCCAagcgagc                                           >  2:554283/1‑140 (MQ=255)
                                                                                              gcgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAagcgagcg                                          >  2:289888/1‑140 (MQ=255)
                                                                                               cgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGt                                         >  1:1013016/1‑140 (MQ=255)
                                                                                               cgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGt                                         >  1:308649/1‑140 (MQ=255)
                                                                                               cgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGt                                         >  1:1001045/1‑140 (MQ=255)
                                                                                               cgACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTCGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGt                                         >  1:1449550/1‑140 (MQ=255)
                                                                                                    ttCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGcc                                    <  1:689616/140‑1 (MQ=255)
                                                                                                     tCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGccc                                   <  2:1137105/140‑1 (MQ=255)
                                                                                                     tCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGccc                                   <  1:639410/140‑1 (MQ=255)
                                                                                                       cATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCgg                                 <  1:1613720/140‑1 (MQ=255)
                                                                                                               aCGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCaaa                         >  2:1130313/1‑140 (MQ=255)
                                                                                                                cGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaac                        >  1:104530/1‑140 (MQ=255)
                                                                                                                cGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaac                        >  1:733000/1‑140 (MQ=255)
                                                                                                                 gTTGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaaca                       >  1:1403802/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  1:1048132/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  2:1248216/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  2:424577/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  2:1437110/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  1:393165/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  1:270364/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  1:816602/1‑140 (MQ=255)
                                                                                                                   tGCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAaacaac                     >  1:887135/1‑140 (MQ=255)
                                                                                                                    gCAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAAcg                    >  1:230515/1‑140 (MQ=255)
                                                                                                                     cAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAAcgc                   >  1:913735/1‑140 (MQ=255)
                                                                                                                     cAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAAcgc                   >  2:434733/1‑140 (MQ=255)
                                                                                                                     cAGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAAcgc                   >  2:1445312/1‑140 (MQ=255)
                                                                                                                      aGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTTAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATTCAAACAAcgcg                  <  2:1375569/140‑1 (MQ=255)
                                                                                                                      aGCCGTTGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAAcgcg                  <  1:792640/140‑1 (MQ=255)
                                                                                                                           ttGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACg             <  1:125523/140‑1 (MQ=255)
                                                                                                                           ttGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACg             <  1:318215/140‑1 (MQ=255)
                                                                                                                           ttGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACg             <  1:365249/140‑1 (MQ=255)
                                                                                                                           ttGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACg             <  1:545931/140‑1 (MQ=255)
                                                                                                                           ttGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACg             <  1:1015560/140‑1 (MQ=255)
                                                                                                                            tGCGAACGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGc            >  2:827706/1‑140 (MQ=255)
                                                                                                                                 aCGCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATc       <  1:384306/140‑1 (MQ=255)
                                                                                                                                   gCCCGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGGTGATCaa     <  1:326884/140‑1 (MQ=255)
                                                                                                                                    cccGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAac    <  1:406318/140‑1 (MQ=255)
                                                                                                                                     ccGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacg   >  1:708348/1‑140 (MQ=255)
                                                                                                                                      cGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga  <  1:5014/140‑1 (MQ=255)
                                                                                                                                      cGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga  <  2:915041/140‑1 (MQ=255)
                                                                                                                                      cGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga  <  1:559921/140‑1 (MQ=255)
                                                                                                                                      cGTTTGCCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga  <  2:961878/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                     
CGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTG‑CCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGA  >  NC_000913/2610397‑2610669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑