Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	347,623	T→C	K274R (AAG→AGG)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	347,623	0	T	C	100.0%	249.1 / NA	69	K274R (AAG→AGG)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/45);  total (24/45)

CGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGC  >  NC_000913/347486‑347757                                                                                                                                          |                                                                                                                                       cgTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACctt                                                                                                                                      >  1:299431/1‑140 (MQ=255)   tGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACctttt                                                                                                                                    >  1:330469/1‑140 (MQ=255)    gggCAATTTCGAACAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGGTGCGACCTTTTg                                                                                                                                   <  2:131555/140‑1 (MQ=255)     ggCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACctttgcc                                                                                                                                  <  2:78268/140‑4 (MQ=255)     ggCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGc                                                                                                                                  >  1:722948/1‑140 (MQ=255)      gCAATTTCGAACAGCCTGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGcc                                                                                                                                 <  1:1630751/140‑1 (MQ=255)      gCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGcc                                                                                                                                 <  2:1136266/140‑1 (MQ=255)      gCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGcc                                                                                                                                 <  1:614694/140‑1 (MQ=255)      gCAATTCCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGcc                                                                                                                                 <  2:1175921/140‑1 (MQ=255)             cGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTcgc                                                                                                                          <  2:330469/140‑1 (MQ=255)               aaCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCgcgc                                                                                                                        <  2:147195/140‑1 (MQ=255)                    cccGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGt                                                                                                                   >  2:925832/1‑140 (MQ=255)                    cccGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGt                                                                                                                   >  1:1491317/1‑140 (MQ=255)                      cGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTgg                                                                                                                 >  1:682680/1‑140 (MQ=255)                          gcgACCTCGGCGGCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCggg                                                                                                             <  2:986693/140‑1 (MQ=255)                          gcgACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCggg                                                                                                             <  2:777418/140‑1 (MQ=255)                              ccTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCaaa                                                                                                         >  1:302829/1‑140 (MQ=255)                              ccTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCaaa                                                                                                         >  2:8865/1‑140 (MQ=255)                                 ccGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAata                                                                                                      <  2:726049/140‑1 (MQ=255)                                   gcgTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATAtt                                                                                                    >  1:398615/1‑140 (MQ=255)                                       cgcgAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCg                                                                                                <  1:1604246/140‑1 (MQ=255)                                        gcgAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTTCCgg                                                                                               >  2:488110/1‑139 (MQ=255)                                         cgAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGAt                                                                                              >  2:694907/1‑140 (MQ=255)                                           aGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGa                                                                                            <  1:269274/140‑1 (MQ=255)                                            gCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGCGGCGGGCAAAATATTCCCGATGaa                                                                                           <  2:1221745/140‑1 (MQ=255)                                                      gCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgc                                                                                 <  2:198184/140‑1 (MQ=255)                                                        cccTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccg                                                                               <  2:1060381/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  1:581114/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  1:1393755/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  2:875046/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  1:1428897/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  1:311599/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  1:1172287/140‑1 (MQ=255)                                                          cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGgccgcc                                                                             <  2:1369265/140‑1 (MQ=255)                                                             ccTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCCGATGAATCGCCCGGGccgcccgc                                                                          >  2:729236/1‑140 (MQ=255)                                                              cTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGct                                                                         <  2:167737/140‑1 (MQ=255)                                                                cATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGctct                                                                       <  2:1610430/140‑1 (MQ=255)                                                                cATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGctct                                                                       >  1:668800/1‑140 (MQ=255)                                                                 aTAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCCCCCGCTCtt                                                                      >  1:422963/1‑140 (MQ=255)                                                                  tAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCttt                                                                     >  1:1490892/1‑140 (MQ=255)                                                                     ccAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGcc                                                                  >  2:612723/1‑140 (MQ=255)                                                                     ccAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGcc                                                                  <  1:1385800/140‑1 (MQ=255)                                                                       aaaCAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCg                                                                <  1:592332/140‑1 (MQ=255)                                                                        aaCAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCCGCTCTTTGCCCgg                                                               >  2:1267032/1‑139 (MQ=255)                                                                        aaCAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCgc                                                               >  2:542473/1‑139 (MQ=255)                                                                          cAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATAATCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTcc                                                             >  1:1377996/1‑140 (MQ=255)                                                                             ttCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAAACGCCCGGGCCGCCAGCCCTTTGCCCGGccccc                                                          >  2:332685/1‑139 (MQ=255)                                                                                tGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGttt                                                       <  1:1015207/140‑1 (MQ=255)                                                                                  cTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTcc                                                     >  1:1365893/1‑140 (MQ=255)                                                                                     ccAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTccccc                                                  <  1:638403/140‑1 (MQ=255)                                                                                     ccAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTccccc                                                  <  1:1299181/140‑1 (MQ=255)                                                                                       agcagcGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtc                                                >  1:759805/1‑140 (MQ=255)                                                                                             gATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATCCCCGATGAATCGCCTGGGCCGCCAGCTTTTTGCCCGTCCCCGTTCCCCCCtcaatcaa                                          <  2:1491317/140‑1 (MQ=255)                                                                                               ttCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCCGATGAAACGCCTGGGCCGCCAGCTCTTTTCCCGTCCCCGATTCCCCCTCCATCAaca                                        >  2:1286552/1‑140 (MQ=255)                                                                                                  ggCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGCCCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTTTTTGCCCGTCCCCGTTTCCCCCTCAATCAACAccg                                     <  2:499584/140‑1 (MQ=255)                                                                                                      aTCGCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACcgccgc                                 <  2:299431/140‑1 (MQ=255)                                                                                                         gCCCCGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCCCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACt                              <  1:1286552/140‑1 (MQ=255)                                                                                                             cGCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAg                          <  2:1365893/140‑1 (MQ=255)                                                                                                              gCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGc                         <  1:488110/140‑1 (MQ=255)                                                                                                              gCAGTTGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGc                         <  1:8865/140‑1 (MQ=255)                                                                                                                aGTTGCCGGCAACAAACGGATGCGCCCTTTTGCCCGGTCGCGCTTCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCgg                       <  2:682680/140‑1 (MQ=255)                                                                                                                   tGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                    <  2:1326238/140‑1 (MQ=255)                                                                                                                   tGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                    <  1:1288814/140‑1 (MQ=255)                                                                                                                   tGACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGCCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                    <  2:398615/140‑1 (MQ=255)                                                                                                                    gACGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCAt                   >  1:1240354/1‑140 (MQ=255)                                                                                                                     aCGGCAACAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAACCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATa                  <  2:1490892/140‑1 (MQ=255)                                                                                                                        gCAAAAAACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAg               <  2:1139046/140‑1 (MQ=255)                                                                                                                          aacaaACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCa             >  2:30237/1‑140 (MQ=255)                                                                                                                          aacaaACGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCCTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCa             >  2:1491714/1‑140 (MQ=255)                                                                                                                                cGGATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAg       <  2:1240354/140‑1 (MQ=255)                                                                                                                                 ggATGCGCCCTTTCGCGCTGTCGCCCTTCGGGGCGGGCAAAATATTCCCGAGGAAGCCCCTGGGCCGCCGGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCACCGCCGCCGCACTGGAGCGGGCATACAGCAAAATAGt      <  2:302829/140‑1 (MQ=255)                                                                                                                                 ggATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCTCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGt      <  1:925832/140‑1 (MQ=255)                                                                                                                                 ggATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGt      <  1:1025288/140‑1 (MQ=255)                                                                                                                                  gATGCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTc     <  2:358800/140‑1 (MQ=255)                                                                                                                                    ggCGACCTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTTTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTg   <  2:502249/139‑1 (MQ=255)                                                                                                                                     gCGACCTTTTGCCCTGCCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCGGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGc  <  1:488599/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGC  >  NC_000913/347486‑347757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑