Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,787	A→G	T227A (ACC→GCC)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,787	0	A	G	100.0%	311.7 / NA	82	T227A (ACC→GCC)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/49);  total (33/49)

GGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAA  >  NC_000913/4217651‑4217921                                                                                                                                         |                                                                                                                                       ggCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGccc                                                                                                                                     <  2:1588773/140‑1 (MQ=255) ggCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGccc                                                                                                                                     <  1:840339/140‑1 (MQ=255)     gTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgct                                                                                                                                 >  1:215751/1‑140 (MQ=255)     gTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgct                                                                                                                                 >  2:1418109/1‑140 (MQ=255)     gTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgct                                                                                                                                 >  1:1341595/1‑140 (MQ=255)     gTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgct                                                                                                                                 >  2:1476576/1‑140 (MQ=255)     gTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgct                                                                                                                                 >  1:1421157/1‑140 (MQ=255)      ttCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgctg                                                                                                                                <  1:108717/140‑1 (MQ=255)      ttCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCctgctg                                                                                                                                <  2:626788/140‑1 (MQ=255)        cACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGt                                                                                                                              <  2:69684/140‑1 (MQ=255)           ttCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGc                                                                                                                           <  1:597146/140‑1 (MQ=255)            tCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTTGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGcc                                                                                                                          <  1:444562/140‑1 (MQ=255)             cGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGccc                                                                                                                         <  2:1017805/140‑1 (MQ=255)               aaGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGt                                                                                                                       <  1:39275/140‑1 (MQ=255)                aGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCCGCTGGTTGCCCgaa                                                                                                                      >  2:166226/1‑138 (MQ=255)                 gATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTAc                                                                                                                     <  1:1476576/140‑1 (MQ=255)                 gATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTAc                                                                                                                     <  1:205150/140‑1 (MQ=255)                  aTCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTAcc                                                                                                                    >  1:628358/1‑140 (MQ=255)                    cAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGa                                                                                                                  >  2:1053348/1‑140 (MQ=255)                     aGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGAt                                                                                                                 >  2:765257/1‑140 (MQ=255)                       cTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGc                                                                                                               >  1:787204/1‑140 (MQ=255)                          gCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGa                                                                                                            >  1:636576/1‑140 (MQ=255)                           cGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGAt                                                                                                           >  2:1515891/1‑140 (MQ=255)                            gTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATg                                                                                                          >  1:1401927/1‑140 (MQ=255)                               aGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                       <  2:1193941/140‑1 (MQ=255)                               aGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                       <  2:1621937/140‑1 (MQ=255)                               aGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                       <  1:419338/140‑1 (MQ=255)                               aGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                       <  2:672186/140‑1 (MQ=255)                               aGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                       <  2:908142/140‑1 (MQ=255)                                 tGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATgcggcg                                                                                                     <  2:783506/140‑1 (MQ=255)                                  gaagaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGcggcgg                                                                                                    <  1:89936/140‑1 (MQ=255)                                    agaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAt                                                                                                  <  1:1048450/140‑1 (MQ=255)                                    agaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAt                                                                                                  <  2:215751/140‑1 (MQ=255)                                    agaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAt                                                                                                  <  1:1477956/140‑1 (MQ=255)                                     gaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATc                                                                                                 >  2:1233865/1‑140 (MQ=255)                                       aaTGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTg                                                                                               <  2:1444311/140‑1 (MQ=255)                                         tGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGAt                                                                                             <  2:1148801/140‑1 (MQ=255)                                           cGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATcc                                                                                           >  2:1263248/1‑139 (MQ=255)                                             gTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCAcc                                                                                         >  2:1503974/1‑140 (MQ=255)                                                acaTGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTcc                                                                                      >  1:672286/1‑140 (MQ=255)                                                 caTGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCg                                                                                     >  1:58006/1‑140 (MQ=255)                                                   tGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGAt                                                                                   >  1:354318/1‑140 (MQ=255)                                                      gcggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGc                                                                                >  2:10765/1‑140 (MQ=255)                                                      gcggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGc                                                                                >  1:1574483/1‑140 (MQ=255)                                                       cggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                               <  1:1418109/140‑1 (MQ=255)                                                       cggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                               <  2:847275/140‑1 (MQ=255)                                                             aGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGt                                                                         <  1:707082/140‑1 (MQ=255)                                                                     tGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGc                                                                 <  1:137416/140‑1 (MQ=255)                                                                         aaCTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAAt                                                             >  1:413229/1‑140 (MQ=255)                                                                          aCTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAAtt                                                            <  2:467756/140‑1 (MQ=255)                                                                               ggAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATttatta                                                       >  2:624567/1‑140 (MQ=255)                                                                                    cTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGc                                                  <  1:1135549/140‑1 (MQ=255)                                                                                      aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGa                                                <  2:1341595/140‑1 (MQ=255)                                                                                      aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGa                                                >  1:1559038/1‑140 (MQ=255)                                                                                      aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGa                                                >  1:1613570/1‑140 (MQ=255)                                                                                      aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGa                                                >  1:527180/1‑140 (MQ=255)                                                                                       ttCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                               <  1:765257/140‑1 (MQ=255)                                                                                       ttCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                               <  2:628358/140‑1 (MQ=255)                                                                                       ttCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                               <  1:1233865/140‑1 (MQ=255)                                                                                       ttCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                               <  2:239821/140‑1 (MQ=255)                                                                                       ttCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                               <  1:1047533/140‑1 (MQ=255)                                                                                         cAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCg                                             <  2:1100668/140‑1 (MQ=255)                                                                                           gAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTa                                           <  1:933176/140‑1 (MQ=255)                                                                                           gAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTa                                           <  1:583164/140‑1 (MQ=255)                                                                                                 ggTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                     >  1:1507023/1‑140 (MQ=255)                                                                                                 ggTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                     <  2:782509/140‑1 (MQ=255)                                                                                                  gTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGa                                    >  1:563648/1‑140 (MQ=255)                                                                                                  gTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGa                                    >  1:48412/1‑140 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGTTGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTAATACCGGCGAGCGTACCAGTGAAGGc                                <  1:1503974/140‑1 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                <  2:998010/140‑1 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                >  1:653577/1‑140 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                <  2:1401927/140‑1 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                <  2:413229/140‑1 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                <  1:935422/140‑1 (MQ=255)                                                                                                      cggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                <  1:275189/140‑1 (MQ=255)                                                                                                       ggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGct                               <  1:1063054/140‑1 (MQ=255)                                                                                                           cgTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                           <  1:10765/140‑1 (MQ=255)                                                                                                            gTCTGGCAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCttcttc                          >  2:181821/1‑140 (MQ=255)                                                                                                                  cAGCTGACGTGACGGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACt                    <  2:1574483/140‑1 (MQ=255)                                                                                                                       gacgtgacgGACGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGc               <  1:390890/140‑1 (MQ=255)                                                                                                                        acgtgacgGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCg              >  2:442762/1‑140 (MQ=255)                                                                                                                             acgGGCGTTCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCAt         >  1:1358960/1‑140 (MQ=255)                                                                                                                                    ttCCAGCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCaa  >  2:310701/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAA  >  NC_000913/4217651‑4217921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑