Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,295,048	C→T	R190C (CGC→TGC)	gltP →	glutamate/aspartate : H(+) symporter GltP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,295,048	0	C	T	100.0%	339.6 / NA	96	R190C (CGC→TGC)	gltP	glutamate/aspartate : H(+) symporter GltP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/66);  total (30/66)

GTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGC  >  NC_000913/4294912‑4295183                                                                                                                                         |                                                                                                                                        gTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCt                                                                                                                                      <  2:1384799/140‑1 (MQ=255) gTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCt                                                                                                                                      <  1:1631698/140‑1 (MQ=255)  tCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTc                                                                                                                                     <  2:452068/140‑1 (MQ=255)   cGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTcc                                                                                                                                    <  1:597321/140‑1 (MQ=255)    gCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCa                                                                                                                                   <  2:363516/140‑1 (MQ=255)    gCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCa                                                                                                                                   <  1:1172011/140‑1 (MQ=255)              acgaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAAc                                                                                                                         <  2:37397/140‑1 (MQ=255)              acgaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAAc                                                                                                                         <  2:60096/140‑1 (MQ=255)               cgaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAAcc                                                                                                                        <  2:37472/140‑1 (MQ=255)               cgaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAAcc                                                                                                                        <  1:1390328/140‑1 (MQ=255)                gaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCa                                                                                                                       <  2:103330/140‑1 (MQ=255)                gaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCa                                                                                                                       <  2:771932/140‑1 (MQ=255)                      tgtgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTa                                                                                                                 <  1:471369/140‑1 (MQ=255)                      tgtgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTa                                                                                                                 <  1:734837/140‑1 (MQ=255)                        tgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTaaa                                                                                                               <  1:766652/140‑1 (MQ=255)                        tgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTaaa                                                                                                               >  2:1632533/1‑140 (MQ=255)                          gCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGt                                                                                                             <  1:1295920/140‑1 (MQ=255)                           cGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTg                                                                                                            <  2:1199090/140‑1 (MQ=255)                            gTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGa                                                                                                           <  1:1057826/140‑1 (MQ=255)                             tCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGAc                                                                                                          <  1:837760/140‑1 (MQ=255)                             tCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGAc                                                                                                          <  1:1432806/140‑1 (MQ=255)                             tCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGAc                                                                                                          >  1:986713/1‑140 (MQ=255)                             tCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGAc                                                                                                          <  1:293313/140‑1 (MQ=255)                               gATGGTGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTc                                                                                                        <  1:1531650/140‑1 (MQ=255)                               gATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTc                                                                                                        <  2:866706/140‑1 (MQ=255)                                aTGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTca                                                                                                       >  2:1554138/1‑140 (MQ=255)                                aTGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTca                                                                                                       >  1:731587/1‑140 (MQ=255)                                 tGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcac                                                                                                      <  2:471953/140‑1 (MQ=255)                                 tGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcac                                                                                                      <  1:992689/140‑1 (MQ=255)                                 tGGCGAAAGGCGAAATGCTGCCGATCAGCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCAGCTCTGAAACCATGTTTAAAGTGACTcac                                                                                                      <  2:1428150/140‑1 (MQ=255)                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                                     <  2:473050/140‑1 (MQ=255)                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                                     <  1:1511360/140‑1 (MQ=255)                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                                     <  1:461140/140‑1 (MQ=255)                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                                     <  1:651295/140‑1 (MQ=255)                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTg                                                                                                <  1:1638424/140‑1 (MQ=255)                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTg                                                                                                <  1:96389/140‑1 (MQ=255)                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTg                                                                                                <  1:1064550/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  2:206654/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  2:227679/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  2:1364954/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  1:110475/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  1:1386095/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  1:1158114/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  1:1297614/140‑1 (MQ=255)                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTACTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                                              <  1:502535/140‑1 (MQ=255)                                              aaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGtt                                                                                         <  2:1321100/140‑1 (MQ=255)                                                  ctgcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGc                                                                                     <  2:137775/140‑1 (MQ=255)                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                                                   <  2:309753/140‑1 (MQ=255)                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                                                   <  2:235911/140‑1 (MQ=255)                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                                                   <  2:1353300/140‑1 (MQ=255)                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                                                   >  1:1545396/1‑140 (MQ=255)                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGCGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                                                   <  1:1504411/140‑1 (MQ=255)                                                     ccGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAcc                                                                                  >  2:286154/1‑140 (MQ=255)                                                      cGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCg                                                                                 <  1:531753/140‑1 (MQ=255)                                                      cGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCg                                                                                 >  1:131232/1‑140 (MQ=255)                                                       gATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCgg                                                                                <  1:641690/140‑1 (MQ=255)                                                        atcatcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggg                                                                               >  2:795053/1‑139 (MQ=255)                                                          catcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggtgg                                                                             <  1:219310/140‑1 (MQ=255)                                                           atcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggtggg                                                                            <  1:134194/140‑1 (MQ=255)                                                             cTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggtgggtg                                                                          <  2:1161862/140‑1 (MQ=255)                                                                   ctcGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTg                                                                    >  2:834038/1‑140 (MQ=255)                                                                   ctcGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTg                                                                    >  1:905711/1‑140 (MQ=255)                                                                    tcGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTgc                                                                   <  2:808796/140‑1 (MQ=255)                                                                        tGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTg                                                               >  1:1251219/1‑140 (MQ=255)                                                                         gCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGa                                                              <  2:986713/140‑1 (MQ=255)                                                                             tttGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGc                                                          >  2:1383277/1‑140 (MQ=255)                                                                                ggTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGt                                                       <  2:1319643/140‑1 (MQ=255)                                                                                ggTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGt                                                       >  2:1111765/1‑140 (MQ=255)                                                                                ggTCAGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGAGTTCTGCTCCATCTCTGAAACCATGCTTAAAATGACTCACATGGTGATGCGTTATGCACCGGCCGGTGTGTTTGCGCTGGTTGCggc                                                       >  2:655825/1‑139 (MQ=255)                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                                     >  2:715513/1‑140 (MQ=255)                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCCGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                                     >  2:538118/1‑140 (MQ=255)                                                                                  tCTGGGGCTTTCCTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                                     >  1:1417521/1‑140 (MQ=255)                                                                                    tGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACg                                                   <  1:578280/140‑1 (MQ=255)                                                                                      gggCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGt                                                 >  1:1029384/1‑140 (MQ=255)                                                                                      gggCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGt                                                 >  2:1308247/1‑140 (MQ=255)                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                                                >  2:1230738/1‑140 (MQ=255)                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                                                >  2:441165/1‑140 (MQ=255)                                                                                            tcttcCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                                           >  2:269162/1‑140 (MQ=255)                                                                                            tcttcCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                                           >  1:664679/1‑140 (MQ=255)                                                                                            tcttcCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                                           >  2:1377075/1‑140 (MQ=255)                                                                                                  cTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTgg                                     >  1:203165/1‑140 (MQ=255)                                                                                                  cTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTgg                                     <  1:102614/140‑1 (MQ=255)                                                                                                     cccGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGttt                                  >  2:756357/1‑140 (MQ=255)                                                                                                      ccGCGACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTtc                                 >  1:31518/1‑140 (MQ=255)                                                                                                        gcgACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTtctc                               >  2:716054/1‑140 (MQ=255)                                                                                                         cgACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCg                              <  1:1554138/140‑1 (MQ=255)                                                                                                          gACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGt                             <  1:776338/140‑1 (MQ=255)                                                                                                          gACGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGt                             <  2:159565/140‑1 (MQ=255)                                                                                                            cGCATCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGtct                           <  1:846725/140‑1 (MQ=255)                                                                                                               aTCGTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTctctt                        >  1:1134476/1‑139 (MQ=255)                                                                                                                  gTGAACCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTgg                     <  1:714444/140‑1 (MQ=255)                                                                                                                     aaCCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCa                  <  2:731587/140‑1 (MQ=255)                                                                                                                      aCCGCTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCAc                 >  2:707098/1‑140 (MQ=255)                                                                                                                          cTGGTGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGc             >  1:1000035/1‑140 (MQ=255)                                                                                                                              tGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGaaa         <  2:42123/140‑1 (MQ=255)                                                                                                                              tGACCGTGTTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGaaa         <  1:715513/140‑1 (MQ=255)                                                                                                                                    tgtTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGtg   >  1:317767/1‑140 (MQ=255)                                                                                                                                     gtTCTGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGtgc  <  1:1377075/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGC  >  NC_000913/4294912‑4295183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑