Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,577	T→C	intergenic (+36/‑4)	rhsD → / → ybbC	protein RhsD/PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,577	0	T	C	100.0%	241.8 / NA	64	intergenic (+36/‑4)	rhsD/ybbC	protein RhsD/PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/25);  total (39/25)

ATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527444‑527713                                                                                                                                      |                                                                                                                                         aTGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATg                                                                                                                                    <  1:832984/140‑1 (MQ=255)     aCGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAat                                                                                                                                <  2:23761/140‑1 (MQ=255)           tGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGtt                                                                                                                          >  2:440486/1‑140 (MQ=255)                  cTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAtt                                                                                                                   >  1:683057/1‑140 (MQ=255)                             aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  2:664797/1‑140 (MQ=255)                             aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  1:963151/1‑140 (MQ=255)                               gagaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCa                                                                                                      >  2:702566/1‑140 (MQ=255)                               gagaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCa                                                                                                      >  2:626363/1‑140 (MQ=255)                                 gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtt                                                                                                    >  2:1272172/1‑140 (MQ=255)                                  aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  1:54046/1‑140 (MQ=255)                                  aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  2:422631/1‑140 (MQ=255)                                  aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  2:187276/1‑140 (MQ=255)                                       atTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTtat                                                                                              <  2:85369/140‑1 (MQ=255)                                            tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAt                                                                                         >  2:335895/1‑140 (MQ=255)                                             aTCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAtt                                                                                        <  1:1071196/140‑1 (MQ=255)                                               cAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTg                                                                                      >  2:549938/1‑140 (MQ=255)                                                aGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGc                                                                                     <  2:1284666/140‑1 (MQ=255)                                                      aGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTaa                                                                               <  2:444555/140‑1 (MQ=255)                                                        tgtCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATg                                                                             >  1:340879/1‑140 (MQ=255)                                                           cccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAACGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATgaga                                                                          <  1:1393661/140‑1 (MQ=255)                                                            ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc                                                                         >  2:1604973/1‑140 (MQ=255)                                                            ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc                                                                         >  2:1030137/1‑140 (MQ=255)                                                            ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc                                                                         >  1:329246/1‑140 (MQ=255)                                                             cGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACa                                                                        <  2:421684/140‑1 (MQ=255)                                                              gggATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAg                                                                       <  2:683543/140‑1 (MQ=255)                                                              gggATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAg                                                                       <  2:268571/140‑1 (MQ=255)                                                                gATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCt                                                                     >  2:1081612/1‑140 (MQ=255)                                                                     aGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa                                                                <  2:192666/140‑1 (MQ=255)                                                                         cAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGt                                                            >  1:583900/1‑140 (MQ=255)                                                                                 aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga                                                    >  2:115770/1‑140 (MQ=255)                                                                                 aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga                                                    >  1:646135/1‑140 (MQ=255)                                                                                    ccGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACCATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa                                                 >  1:1607039/1‑140 (MQ=255)                                                                                     cGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAAc                                                >  1:1073695/1‑140 (MQ=255)                                                                                     cGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAAc                                                >  2:1415195/1‑140 (MQ=255)                                                                                      gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  1:1468148/1‑140 (MQ=255)                                                                                      gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  2:1457668/1‑140 (MQ=255)                                                                                      gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  2:308238/1‑140 (MQ=255)                                                                                         tCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            <  2:464798/140‑1 (MQ=255)                                                                                          cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattat                                           >  1:361689/1‑140 (MQ=255)                                                                                          cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattat                                           >  2:1068909/1‑140 (MQ=255)                                                                                             gATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTAtttt                                        >  1:917459/1‑140 (MQ=255)                                                                                              aTTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTAttttt                                       >  2:1348929/1‑140 (MQ=255)                                                                                               ttAATTTTAGTGTTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa                                      >  2:367135/1‑140 (MQ=255)                                                                                               ttAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa                                      >  2:1318093/1‑140 (MQ=255)                                                                                                   ttttAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAg                                  >  2:1427830/1‑140 (MQ=255)                                                                                                         tGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTAtgt                            >  2:273145/1‑140 (MQ=255)                                                                                                                     tGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACa                <  1:266659/140‑1 (MQ=255)                                                                                                                       gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:646135/140‑1 (MQ=255)                                                                                                                       gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:422631/140‑1 (MQ=255)                                                                                                                       gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:335895/140‑1 (MQ=255)                                                                                                                        ggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt             >  2:440233/1‑140 (MQ=255)                                                                                                                         gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTa            >  1:912347/1‑140 (MQ=255)                                                                                                                         gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTa            <  2:583900/140‑1 (MQ=255)                                                                                                                          ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg           >  1:764058/1‑140 (MQ=255)                                                                                                                          ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg           >  2:1214923/1‑140 (MQ=255)                                                                                                                                  gagaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAc   <  1:626363/140‑1 (MQ=255)                                                                                                                                  gagaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAc   <  1:549938/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  >  1:1581097/1‑140 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  1:21683/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  1:367135/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:683057/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:361689/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  1:1081612/140‑1 (MQ=255)                                                                                                                                   agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:329246/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527444‑527713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑