Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	957,271	A→G	K170K (AAA→AAG)	ycaP →	DUF421 domain‑containing protein YcaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	957,271	0	A	G	100.0%	323.7 / NA	86	K170K (AAA→AAG)	ycaP	DUF421 domain‑containing protein YcaP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/48);  total (38/48)

CTCCAACATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAACCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCGCGCTGTG  >  NC_000913/957136‑957407                                                                                                                                        |                                                                                                                                         cTCCAACATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCgg                                                                                                                                      <  1:1550138/140‑1 (MQ=255) cTCCAACATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCgg                                                                                                                                      >  2:1295990/1‑140 (MQ=255)      aCATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTa                                                                                                                                 >  2:1171874/1‑140 (MQ=255)       cATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTAc                                                                                                                                <  1:556497/140‑1 (MQ=255)       cATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTAc                                                                                                                                <  2:1045662/140‑1 (MQ=255)       cATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTAc                                                                                                                                <  2:172281/140‑1 (MQ=255)       cATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTAc                                                                                                                                <  2:390508/140‑1 (MQ=255)            cGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTAtt                                                                                                                           >  1:692186/1‑140 (MQ=255)                    gAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAg                                                                                                                   <  2:489199/140‑1 (MQ=255)                    gAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAg                                                                                                                   <  1:288522/140‑1 (MQ=255)                      gTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTg                                                                                                                 <  2:1155516/140‑1 (MQ=255)                       ttCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGa                                                                                                                <  2:1198658/140‑1 (MQ=255)                       ttCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGa                                                                                                                >  2:493192/1‑140 (MQ=255)                              tGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACg                                                                                                         <  2:1535653/140‑1 (MQ=255)                              tGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACg                                                                                                         <  2:1323241/140‑1 (MQ=255)                              tGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACg                                                                                                         <  2:1177920/140‑1 (MQ=255)                                gAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCa                                                                                                       <  2:349173/140‑1 (MQ=255)                                 aGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAg                                                                                                      <  1:171869/140‑1 (MQ=255)                                 aGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAg                                                                                                      <  1:1109916/140‑1 (MQ=255)                                   cTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCg                                                                                                    >  1:174112/1‑140 (MQ=255)                                   cTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCg                                                                                                    >  1:1050702/1‑140 (MQ=255)                                   cTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCg                                                                                                    >  1:1319843/1‑140 (MQ=255)                                    tACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGt                                                                                                   >  2:1211726/1‑140 (MQ=255)                                    tACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGt                                                                                                   >  1:1410680/1‑140 (MQ=255)                                    tACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGt                                                                                                   >  2:1840/1‑140 (MQ=255)                                    tACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGt                                                                                                   >  2:1089887/1‑140 (MQ=255)                                    tACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGt                                                                                                   >  1:1054566/1‑140 (MQ=255)                                      cGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTa                                                                                                 >  2:997319/1‑140 (MQ=255)                                           gcgtggcgtgGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAg                                                                                            >  1:371094/1‑140 (MQ=255)                                           gcgtggcgtgGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAg                                                                                            >  1:1343329/1‑140 (MQ=255)                                           gcgtggcgtgGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAg                                                                                            >  2:1271955/1‑140 (MQ=255)                                              tggcgtggAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAgtgg                                                                                         >  1:1432719/1‑140 (MQ=255)                                                gcgtggAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAgtggtg                                                                                       <  1:612705/140‑1 (MQ=255)                                                  gtggAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGcc                                                                                     <  2:919738/140‑1 (MQ=255)                                                     gAGCAGATGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGa                                                                                  <  1:1345275/140‑1 (MQ=255)                                                          gcTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTcgg                                                                             <  2:478682/140‑1 (MQ=255)                                                           cTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTcggc                                                                            >  2:1453256/1‑140 (MQ=255)                                                              gggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGa                                                                         <  1:855346/140‑1 (MQ=255)                                                              gggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGa                                                                         <  1:1429551/140‑1 (MQ=255)                                                              gggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGa                                                                         <  1:1295990/140‑1 (MQ=255)                                                              gggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGCTACAAAGTGGTGCCGGAGTCGGCGGa                                                                         <  2:1319843/140‑1 (MQ=255)                                                               ggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGAc                                                                        <  2:51644/140‑1 (MQ=255)                                                               ggCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGAc                                                                        >  1:304171/1‑140 (MQ=255)                                                                gCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACt                                                                       <  1:1058730/140‑1 (MQ=255)                                                                gCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACt                                                                       <  1:614911/140‑1 (MQ=255)                                                                   ggTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATg                                                                    <  1:493192/140‑1 (MQ=255)                                                                     tACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGcc                                                                  <  1:1089887/140‑1 (MQ=255)                                                                     tACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGcc                                                                  >  1:207537/1‑140 (MQ=255)                                                                     tACGCCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGcc                                                                  <  2:1217807/140‑1 (MQ=255)                                                                      aCGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCt                                                                 <  1:1840/140‑1 (MQ=255)                                                                        gTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGt                                                               >  2:611403/1‑140 (MQ=255)                                                                        gTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGt                                                               <  1:295391/140‑1 (MQ=255)                                                                         tCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTa                                                              <  2:1050702/140‑1 (MQ=255)                                                                         tCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTa                                                              >  1:332285/1‑140 (MQ=255)                                                                          cTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTAt                                                             >  2:346247/1‑140 (MQ=255)                                                                          cTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTAt                                                             >  2:1499011/1‑140 (MQ=255)                                                                             gCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCg                                                          <  2:452199/140‑1 (MQ=255)                                                                                   ctcGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAg                                                    <  2:10483/140‑1 (MQ=255)                                                                                   ctcGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAg                                                    <  1:788047/140‑1 (MQ=255)                                                                                   ctcGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAg                                                    >  1:62209/1‑140 (MQ=255)                                                                                       aaaCCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGaa                                                <  2:174112/140‑1 (MQ=255)                                                                                       aaaCCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGaa                                                >  2:88064/1‑140 (MQ=255)                                                                                         aCCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAt                                              <  1:817985/140‑1 (MQ=255)                                                                                            aaCGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcat                                           <  1:1171874/140‑1 (MQ=255)                                                                                            aaCGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcat                                           <  1:478160/140‑1 (MQ=255)                                                                                            aaCGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcat                                           >  1:776300/1‑140 (MQ=255)                                                                                            aaCGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcat                                           <  2:1258840/140‑1 (MQ=255)                                                                                             aCGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcatt                                          <  2:1016316/140‑1 (MQ=255)                                                                                              cGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAAtcattc                                         >  1:986311/1‑140 (MQ=255)                                                                                                    aaTCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGa                                   >  1:1188480/1‑140 (MQ=255)                                                                                                     aTCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGaa                                  >  2:1253069/1‑140 (MQ=255)                                                                                                          tgtCTATTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCgg                             >  2:103922/1‑140 (MQ=255)                                                                                                               aTTTCTTTGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGaa                        <  2:1432719/140‑1 (MQ=255)                                                                                                                      tGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAAt                 <  1:408059/140‑1 (MQ=255)                                                                                                                      tGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAAt                 >  1:715012/1‑140 (MQ=255)                                                                                                                      tGAAGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGCGAAATCATTCATATGAAAGCGGGGGAAAAACAAt                 >  2:496502/1‑140 (MQ=255)                                                                                                                        aaGATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTa               >  1:1422722/1‑140 (MQ=255)                                                                                                                          gATGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATg             >  2:1133906/1‑140 (MQ=255)                                                                                                                           aTGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGt            <  2:1054566/140‑1 (MQ=255)                                                                                                                           aTGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGt            <  2:1410680/140‑1 (MQ=255)                                                                                                                            tGACAAGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTc           >  2:1294974/1‑140 (MQ=255)                                                                                                                                 aGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCgcgc      <  2:207537/140‑1 (MQ=255)                                                                                                                                 aGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCgcgc      <  1:997319/140‑1 (MQ=255)                                                                                                                                 aGGTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCgcgc      <  1:1505324/140‑1 (MQ=255)                                                                                                                                  ggTGAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCGCGCt     >  1:990820/1‑140 (MQ=255)                                                                                                                                     gAAGCCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCGCGCtgtg  >  2:1046337/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CTCCAACATGACGGAATTTGAGTTCTTTATGGAGCTACGATTGCGTGGCGTGGAGCAGCTGGGGCAGGTACGTCTGGCGATTCTCGAAACCAACGGGCAAATCAGTGTCTATTTCTTTGAAGATGACAAGGTGAAACCGGGTTTACTTATTTTACCCAGTGATTGTACGCAGCGTTACAAAGTGGTGCCGGAGTCGGCGGACTATGCCTGTATTCGTTGTAGTGAAATCATTCATATGAAAGCGGGGGAAAAACAATTATGTCCGCGCTGTG  >  NC_000913/957136‑957407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑