BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	167,157	C→T	Q810* (CAG→TAG)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	167,157	0	C	T	100.0%	442.5 / NA	125	Q810* (CAG→TAG)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (60/65); total (60/65)

Only 100 of 125 total aligned reads displayed.

CAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGA  >  NC_000913/167020‑167292

cAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAg                                                                                                                                       <  2:163979/140‑1 (MQ=255)

 aGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGt                                                                                                                                      >  2:1466957/1‑140 (MQ=255)

   atatGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGtct                                                                                                                                    >  2:1459804/1‑140 (MQ=255)

   atatGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGtct                                                                                                                                    >  1:1151830/1‑140 (MQ=255)

    tatGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGtctt                                                                                                                                   >  2:1212444/1‑140 (MQ=255)

     atGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGtcttc                                                                                                                                  >  2:57048/1‑140 (MQ=255)

     atGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGtcttc                                                                                                                                  <  2:808702/140‑1 (MQ=255)

        ggCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCa                                                                                                                               >  1:991960/1‑140 (MQ=255)

          cGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGc                                                                                                                             <  1:1363445/140‑1 (MQ=255)

             ggACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgc                                                                                                                          >  2:317493/1‑140 (MQ=255)

             ggACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgc                                                                                                                          >  1:885842/1‑140 (MQ=255)

             ggACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgc                                                                                                                          >  1:1157634/1‑140 (MQ=255)

             ggACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgc                                                                                                                          >  2:1513623/1‑140 (MQ=255)

                 tACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgcagca                                                                                                                      <  2:1085433/140‑1 (MQ=255)

                 tACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCgcagca                                                                                                                      <  1:827268/140‑1 (MQ=255)

                  acgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCaa                                                                                                                     >  1:1213306/1‑140 (MQ=255)

                   cgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAAc                                                                                                                    >  2:1181984/1‑140 (MQ=255)

                   cgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAAc                                                                                                                    >  2:542534/1‑140 (MQ=255)

                   cgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAAc                                                                                                                    >  2:1605762/1‑140 (MQ=255)

                   cgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAAc                                                                                                                    >  1:1140199/1‑140 (MQ=255)

                   cgacgGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAAc                                                                                                                    >  2:1098259/1‑140 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               >  2:1496342/1‑140 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               >  2:1363015/1‑140 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               <  1:1406649/140‑1 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               >  2:244001/1‑140 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               <  1:87451/140‑1 (MQ=255)

                        gCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCCGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCg                                                                                                               <  1:1505562/140‑1 (MQ=255)

                         cAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGc                                                                                                              >  1:274037/1‑140 (MQ=255)

                         cAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGc                                                                                                              >  2:1126366/1‑140 (MQ=255)

                         cAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGc                                                                                                              >  1:36919/1‑140 (MQ=255)

                         cAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGc                                                                                                              >  1:1421930/1‑140 (MQ=255)

                             tttgtttgCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACa                                                                                                          <  2:1105963/140‑1 (MQ=255)

                             tttgtttgCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACa                                                                                                          <  2:1292330/140‑1 (MQ=255)

                             tttgtttgCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACa                                                                                                          <  2:238872/140‑1 (MQ=255)

                                gtttgCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCa                                                                                                       >  2:594144/1‑140 (MQ=255)

                                   tgCGGCGGTGGCTTGCGTATCTTGCCGGTCTGGACCAGCGTTCCGCAATCGCTGTGCCAGCAGAGCGAGAGGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAAcc                                                                                                    <  2:536612/140‑1 (MQ=255)

                                    gCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCt                                                                                                   >  2:1323230/1‑140 (MQ=255)

                                      aGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGc                                                                                                 <  1:1407843/140‑1 (MQ=255)

                                           tGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGc                                                                                            <  1:1568384/140‑1 (MQ=255)

                                            ggCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCa                                                                                           <  1:1583176/140‑1 (MQ=255)

                                            ggCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCa                                                                                           <  1:682435/140‑1 (MQ=255)

                                              cATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAag                                                                                         <  1:553027/140‑1 (MQ=255)

                                                tGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAagag                                                                                       <  2:419678/140‑1 (MQ=255)

                                                  cGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCa                                                                                     >  1:448115/1‑140 (MQ=255)

                                                   gTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAg                                                                                    <  2:1233319/140‑1 (MQ=255)

                                                     aTCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGaa                                                                                  >  2:1472369/1‑140 (MQ=255)

                                                       cTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGATTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCGCAGCAAGAGCAGAAAg                                                                                >  1:201655/1‑140 (MQ=255)

                                                          gCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAGCAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACa                                                                             >  1:1378514/1‑140 (MQ=255)

                                                             ggTCTGGTCCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCGGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCg                                                                          <  1:1600811/140‑1 (MQ=255)

                                                             ggTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCg                                                                          <  1:1466957/140‑1 (MQ=255)

                                                              gTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGa                                                                         <  1:1513623/140‑1 (MQ=255)

                                                              gTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGa                                                                         <  2:613795/140‑1 (MQ=255)

                                                              gTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGa                                                                         <  2:1421930/140‑1 (MQ=255)

                                                               tCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGAc                                                                        <  1:1284356/140‑1 (MQ=255)

                                                               tCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGAc                                                                        >  2:63911/1‑140 (MQ=255)

                                                                   gACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGgtg                                                                    >  2:1011999/1‑140 (MQ=255)

                                                                        gCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGcc                                                               >  2:277653/1‑140 (MQ=255)

                                                                         cGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCg                                                              >  2:420218/1‑140 (MQ=255)

                                                                           aTCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGt                                                            <  1:868551/140‑1 (MQ=255)

                                                                           aTCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGt                                                            <  2:1437043/140‑1 (MQ=255)

                                                                           aTCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGt                                                            <  2:722663/140‑1 (MQ=255)

                                                                           aTCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGt                                                            <  2:950611/140‑1 (MQ=255)

                                                                             ccGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTg                                                          >  2:957843/1‑140 (MQ=255)

                                                                             ccGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTg                                                          <  2:750771/140‑1 (MQ=255)

                                                                               gCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGa                                                        <  2:293314/140‑1 (MQ=255)

                                                                                 aaTCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATc                                                      <  2:713639/140‑1 (MQ=255)

                                                                                   tCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCaa                                                    >  1:159056/1‑140 (MQ=255)

                                                                                     gCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAgg                                                  <  1:110455/140‑1 (MQ=255)

                                                                                      cTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGa                                                 >  1:744056/1‑140 (MQ=255)

                                                                                      cTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGa                                                 >  1:564931/1‑140 (MQ=255)

                                                                                       tgtgCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGat                                                <  2:691823/140‑1 (MQ=255)

                                                                                       tgtgCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGat                                                <  1:1459804/140‑1 (MQ=255)

                                                                                       tgtgCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGat                                                <  1:594144/140‑1 (MQ=255)

                                                                                        gtgCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGata                                               >  1:1049694/1‑140 (MQ=255)

                                                                                           ccAGCAGAGCGAGATGCAGCAGCGGCCGTCAGGCAATCCGTTTGATTAGTCTTCGCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGGCGGTGTAGCCGGTTGGATCAAGGATATGt                                            <  1:357902/140‑1 (MQ=255)

                                                                                                 gagCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgta                                      >  2:511093/1‑140 (MQ=255)

                                                                                                  agCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtag                                     <  1:1011999/140‑1 (MQ=255)

                                                                                                  agCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtag                                     <  1:1640760/140‑1 (MQ=255)

                                                                                                  agCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtag                                     <  1:542534/140‑1 (MQ=255)

                                                                                                   gCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagt                                    >  2:416875/1‑140 (MQ=255)

                                                                                                   gCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagt                                    >  2:673829/1‑140 (MQ=255)

                                                                                                   gCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagt                                    >  2:487856/1‑140 (MQ=255)

                                                                                                    cGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagta                                   >  1:928852/1‑140 (MQ=255)

                                                                                                    cGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagta                                   <  1:1126366/140‑1 (MQ=255)

                                                                                                    cGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgtagta                                   >  1:1599740/1‑140 (MQ=255)

                                                                                                     gagaTGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTaa                                  >  1:192516/1‑140 (MQ=255)

                                                                                                      agaTGCAGCAGCAGCCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAAt                                 <  2:446724/140‑1 (MQ=255)

                                                                                                                   gcCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCg                    >  1:551156/1‑140 (MQ=255)

                                                                                                                   gcCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCg                    >  1:1055110/1‑140 (MQ=255)

                                                                                                                   gcCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCg                    >  1:928730/1‑140 (MQ=255)

                                                                                                                   gcCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCg                    <  2:297220/140‑1 (MQ=255)

                                                                                                                   gcCGTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCACAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCg                    >  2:1516371/1‑140 (MQ=255)

                                                                                                                      gTCAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGa                 >  1:679085/1‑140 (MQ=255)

                                                                                                                        cAGGCAATCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGaaa               >  2:1429464/1‑140 (MQ=255)

                                                                                                                               tCCGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGa        <  1:1605762/140‑1 (MQ=255)

                                                                                                                                 cGTTTGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATg      <  1:1028097/140‑1 (MQ=255)

                                                                                                                                   tttGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGc    <  1:1212444/140‑1 (MQ=255)

                                                                                                                                   tttGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGc    >  1:369994/1‑140 (MQ=255)

                                                                                                                                    ttGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCg   <  2:1213306/140‑1 (MQ=255)

                                                                                                                                     tGATTAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGa  >  1:1380643/1‑140 (MQ=255)

CAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGA  >  NC_000913/167020‑167292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`