Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,996	T→C	T461A (ACA→GCA)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,996	0	T	C	100.0%	256.3 / NA	70	T461A (ACA→GCA)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/34);  total (36/34)

CCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTC  >  NC_000913/1245861‑1246132                                                                                                                                        |                                                                                                                                         ccGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCcgcg                                                                                                                                      <  2:539896/140‑1 (MQ=255)  cGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGa                                                                                                                                     <  1:246705/140‑1 (MQ=255)    ttCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGAcc                                                                                                                                   <  2:1202170/140‑1 (MQ=255)    ttCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGAcc                                                                                                                                   <  2:831991/140‑1 (MQ=255)    ttCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGAcc                                                                                                                                   <  2:1109864/140‑1 (MQ=255)    ttCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGAcc                                                                                                                                   <  1:484494/140‑1 (MQ=255)    ttCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGAcc                                                                                                                                   <  1:827262/140‑1 (MQ=255)         gtggtgCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCTCCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTg                                                                                                                              <  1:35590/140‑1 (MQ=255)           ggtgCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTa                                                                                                                            >  2:779027/1‑140 (MQ=255)              gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACg                                                                                                                         >  1:185655/1‑140 (MQ=255)              gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACg                                                                                                                         >  2:258011/1‑140 (MQ=255)              gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACg                                                                                                                         <  1:719960/140‑1 (MQ=255)               cTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACgg                                                                                                                        >  1:368347/1‑140 (MQ=255)                      catATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAg                                                                                                                 >  2:1511148/1‑140 (MQ=255)                           ttttCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCAtt                                                                                                            <  1:477436/140‑1 (MQ=255)                               ccaccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgc                                                                                                        <  1:1561566/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  2:1528335/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  1:1141232/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  1:946321/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  2:1612834/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  2:1212851/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  1:1432074/140‑1 (MQ=255)                                caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                       <  1:336247/140‑1 (MQ=255)                                  ccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCa                                                                                                     <  2:1526614/140‑1 (MQ=255)                                               ccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGcc                                                                                        <  2:315049/140‑1 (MQ=255)                                               ccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGcc                                                                                        >  1:881993/1‑140 (MQ=255)                                                         gtgtGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc                                                                              <  1:80894/140‑1 (MQ=255)                                                           gtgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCg                                                                            <  2:1223153/140‑1 (MQ=255)                                                            tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGa                                                                           >  1:1369114/1‑140 (MQ=255)                                                            tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGa                                                                           >  2:1609401/1‑140 (MQ=255)                                                            tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGa                                                                           >  2:690660/1‑140 (MQ=255)                                                            tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGa                                                                           >  2:1547205/1‑140 (MQ=255)                                                            tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGa                                                                           >  1:979000/1‑140 (MQ=255)                                                             gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGAc                                                                          >  1:163315/1‑140 (MQ=255)                                                             gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGAc                                                                          >  1:697088/1‑140 (MQ=255)                                                             gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGAc                                                                          >  1:555610/1‑140 (MQ=255)                                                             gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGAc                                                                          >  2:187065/1‑140 (MQ=255)                                                              cTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACg                                                                         <  1:523457/140‑1 (MQ=255)                                                              cTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACg                                                                         >  2:1113062/1‑140 (MQ=255)                                                                gAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtg                                                                       <  2:903421/140‑1 (MQ=255)                                                                 aaCATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtgg                                                                      >  2:758781/1‑140 (MQ=255)                                                                 aaCATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtgg                                                                      >  1:991528/1‑140 (MQ=255)                                                                          cAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgc                                                             <  2:368347/140‑1 (MQ=255)                                                                             gAAGTTCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgccgc                                                          <  1:592231/140‑1 (MQ=255)                                                                                   ccAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGtt                                                    >  2:1065851/1‑140 (MQ=255)                                                                                   ccAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGtt                                                    >  1:384362/1‑140 (MQ=255)                                                                                   ccAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGtt                                                    >  2:602131/1‑140 (MQ=255)                                                                                   ccAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGtt                                                    >  2:581518/1‑140 (MQ=255)                                                                                      gCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgca                                                 <  1:743871/140‑1 (MQ=255)                                                                                      gCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgca                                                 <  2:173410/140‑1 (MQ=255)                                                                                          aTGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGcagcag                                             <  2:218138/140‑1 (MQ=255)                                                                                                  cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGttt                                     >  2:593294/1‑140 (MQ=255)                                                                                                      aCCTCTTTTTGCCCGTATTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgt                                 >  2:709663/1‑140 (MQ=255)                                                                                                       ccTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                >  1:1325010/1‑140 (MQ=255)                                                                                                       ccTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                >  1:532022/1‑140 (MQ=255)                                                                                                        ctctTTTTGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                               >  1:432442/1‑140 (MQ=255)                                                                                                            ttttGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                           <  2:1330807/140‑1 (MQ=255)                                                                                                            ttttGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                           <  1:1201615/140‑1 (MQ=255)                                                                                                               tGCCCGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcgc                        >  2:526677/1‑140 (MQ=255)                                                                                                                 cccGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCa                      >  1:1541270/1‑140 (MQ=255)                                                                                                                 cccGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCa                      >  1:688260/1‑140 (MQ=255)                                                                                                                  ccGTAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                     >  1:499834/1‑140 (MQ=255)                                                                                                                     tAGTTTTGTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                  >  1:1587674/1‑140 (MQ=255)                                                                                                                            gTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg           <  2:979000/140‑1 (MQ=255)                                                                                                                            gTAATCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg           <  1:758781/140‑1 (MQ=255)                                                                                                                                tCCTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGTCGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt       >  1:1054944/1‑140 (MQ=255)                                                                                                                                  cTTCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGTAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCt     <  2:1317442/140‑1 (MQ=255)                                                                                                                                    tCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt   >  2:39959/1‑140 (MQ=255)                                                                                                                                    tCTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt   >  1:530482/1‑140 (MQ=255)                                                                                                                                     cTGCCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTc  >  2:957857/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTC  >  NC_000913/1245861‑1246132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑