Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,280,202	G→A	M113I (ATG→ATA)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,280,202	0	G	A	100.0%	287.2 / NA	82	M113I (ATG→ATA)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/36);  total (46/36)

GTCACCTGGGAAACCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAT  >  NC_000913/1280068‑1280335                                                                                                                                       |                                                                                                                                      gTCACCTGGGAAACCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATgcg                                                                                                                                  >  1:662219/1‑140 (MQ=255)        gggAAACCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGc                                                                                                                           <  2:714316/140‑1 (MQ=255)           aaaCCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCtg                                                                                                                        >  1:743304/1‑140 (MQ=255)            aaCCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCtga                                                                                                                       <  2:105294/140‑1 (MQ=255)              cccAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCtgatg                                                                                                                     >  2:985040/1‑140 (MQ=255)              cccAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCtgatg                                                                                                                     >  2:1574299/1‑140 (MQ=255)               ccAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCtgatga                                                                                                                    >  2:215807/1‑140 (MQ=255)                cagcagACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGaa                                                                                                                   >  1:1212725/1‑140 (MQ=255)                cagcagACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGaa                                                                                                                   >  1:802036/1‑140 (MQ=255)                   cagACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAAt                                                                                                                >  2:760047/1‑140 (MQ=255)                   cagACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAAt                                                                                                                >  1:1356038/1‑140 (MQ=255)                         gactATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCg                                                                                                          <  1:1347550/140‑1 (MQ=255)                             aTCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCAGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGaa                                                                                                      >  1:191923/1‑140 (MQ=255)                                cgcgTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCg                                                                                                   >  1:1194970/1‑140 (MQ=255)                                    tgcccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAgg                                                                                               <  1:1416396/138‑1 (MQ=255)                                    tACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAgg                                                                                               <  1:1079809/140‑1 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             <  1:941219/140‑1 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             <  1:1531587/140‑1 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             <  1:834234/140‑1 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             >  2:390185/1‑140 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             >  2:456697/1‑140 (MQ=255)                                      cccGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGgcg                                                                                             >  2:650731/1‑140 (MQ=255)                                        cGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCt                                                                                           >  1:1019910/1‑140 (MQ=255)                                              gATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAg                                                                                     <  2:391803/140‑1 (MQ=255)                                                 cTGCCAAACCATGACCCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGa                                                                                  <  1:1315762/140‑1 (MQ=255)                                                 cTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGa                                                                                  <  2:743304/140‑1 (MQ=255)                                                  tGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGAt                                                                                 <  1:893252/140‑1 (MQ=255)                                                  tGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGAt                                                                                 <  2:607241/140‑1 (MQ=255)                                                  tGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGAt                                                                                 <  1:416585/140‑1 (MQ=255)                                                   gCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATc                                                                                <  1:975026/140‑1 (MQ=255)                                                           aTGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAg                                                                        >  1:123675/1‑140 (MQ=255)                                                              acccTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATGTTTACAGTGCCAACCGCCTGAAATGCCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCa                                                                     <  1:1324953/138‑1 (MQ=255)                                                              aaCCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCa                                                                     >  1:991944/1‑140 (MQ=255)                                                              aaCCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCa                                                                     <  1:635937/140‑1 (MQ=255)                                                              aaCCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCa                                                                     <  2:913184/140‑1 (MQ=255)                                                                ccTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATg                                                                   <  1:174473/140‑1 (MQ=255)                                                                ccTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATg                                                                   <  2:769861/140‑1 (MQ=255)                                                                  tCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATggg                                                                 <  2:662219/140‑1 (MQ=255)                                                                   cgcgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGc                                                                >  2:929408/1‑140 (MQ=255)                                                                   cgcgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGc                                                                >  1:1045904/1‑140 (MQ=255)                                                                   cgcgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGc                                                                >  1:1250546/1‑140 (MQ=255)                                                                   cgcgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGc                                                                <  1:583252/140‑1 (MQ=255)                                                                     cgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCtt                                                              <  1:902277/140‑1 (MQ=255)                                                                     cgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCtt                                                              <  1:1325710/140‑1 (MQ=255)                                                                     cgGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCtt                                                              <  1:666820/140‑1 (MQ=255)                                                                           cccGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCa                                                        >  1:865476/1‑140 (MQ=255)                                                                                gcgGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGaa                                                   <  1:953839/140‑1 (MQ=255)                                                                                  ggTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGa                                                 <  1:985040/140‑1 (MQ=255)                                                                                         gCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGAt                                          >  1:1001914/1‑140 (MQ=255)                                                                                              tCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                     >  1:966110/1‑140 (MQ=255)                                                                                              tCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                     >  1:1643165/1‑140 (MQ=255)                                                                                              tCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                     >  1:1463389/1‑140 (MQ=255)                                                                                              tCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                     >  2:1130065/1‑140 (MQ=255)                                                                                              tCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                     >  1:1133602/1‑140 (MQ=255)                                                                                                cTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGa                                   >  2:78446/1‑140 (MQ=255)                                                                                                 tGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGaa                                  <  1:760047/140‑1 (MQ=255)                                                                                                 tGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGaa                                  <  1:1574299/140‑1 (MQ=255)                                                                                                 tGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGaa                                  <  2:1019910/140‑1 (MQ=255)                                                                                                        tttACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCttt                           >  1:1378108/1‑140 (MQ=255)                                                                                                         ttACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCCGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTa                          >  1:172446/1‑140 (MQ=255)                                                                                                          tACAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTaa                         >  1:347620/1‑140 (MQ=255)                                                                                                           aCAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAg                        >  2:1112087/1‑140 (MQ=255)                                                                                                           aCAGTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAg                        <  2:191923/140‑1 (MQ=255)                                                                                                              gTGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAg                     >  2:1323130/1‑140 (MQ=255)                                                                                                               tGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAgg                    >  1:1588896/1‑140 (MQ=255)                                                                                                               tGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAgg                    >  1:1402006/1‑140 (MQ=255)                                                                                                               tGCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAgg                    <  2:1356038/140‑1 (MQ=255)                                                                                                                gCCAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGgc                   >  1:1354033/1‑140 (MQ=255)                                                                                                                 ccAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGgcg                  >  2:499545/1‑140 (MQ=255)                                                                                                                 ccAACCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGgcg                  >  2:1062257/1‑140 (MQ=255)                                                                                                                    aCCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGt               >  2:395717/1‑140 (MQ=255)                                                                                                                    aCCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGt               >  1:1581868/1‑140 (MQ=255)                                                                                                                    aCCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGt               >  2:1575903/1‑140 (MQ=255)                                                                                                                    aCCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGt               >  1:1297712/1‑140 (MQ=255)                                                                                                                    aCCGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAAAGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGt               >  2:1280271/1‑140 (MQ=255)                                                                                                                     ccGCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTg              <  2:1643165/140‑1 (MQ=255)                                                                                                                       gCCTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGa            <  2:568549/140‑1 (MQ=255)                                                                                                                         cTGAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGAcg          >  2:555664/1‑140 (MQ=255)                                                                                                                          cgAAATCCCCGATAATGCGCAAGCGCGTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGAcgc         <  2:1068904/139‑1 (MQ=255)                                                                                                                           gAAATACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGAcgcg        <  1:1445867/140‑1 (MQ=255)                                                                                                                            aaaTACCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCgg       <  2:1045904/140‑1 (MQ=255)                                                                                                                                aCCCGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGGGGa   >  1:273521/1‑140 (MQ=255)                                                                                                                                 cccGATAATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAt  >  2:505514/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GTCACCTGGGAAACCCAGCAGACTGACTATCCGCGTACCCGTCCGGATCTGCCAAACCATGAACCTCGCGGCTGCCCGCGCGGTGCCAGCTACTCCTGGTATCTTTACAGTGCCAACCGCCTGAAATACCCGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAT  >  NC_000913/1280068‑1280335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑