Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,430,008	C→T	A320A (GCC→GCT)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,430,008	0	C	T	100.0%	144.9 / NA	44	A320A (GCC→GCT)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

ACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAACGCG  >  NC_000913/1429883‑1430143                                                                                                                              |                                                                                                                                        aCGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGaaa                                                                                                                           <  1:1641153/140‑1 (MQ=255)     aCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCg                                                                                                                       >  2:1583680/1‑140 (MQ=255)      cGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCgg                                                                                                                      >  1:1200526/1‑140 (MQ=255)      cGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCgg                                                                                                                      >  2:551912/1‑140 (MQ=255)              cttctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAgc                                                                                                              >  1:1600226/1‑140 (MQ=255)                 ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  1:1324742/1‑140 (MQ=255)                 ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  2:1605065/1‑140 (MQ=255)                 ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  1:438564/1‑140 (MQ=255)                      aCGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATc                                                                                                      >  2:987953/1‑140 (MQ=255)                       cGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCg                                                                                                     >  1:985204/1‑140 (MQ=255)                        ggCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                                                    <  2:1297944/140‑1 (MQ=255)                         gCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTc                                                                                                   <  2:1404691/140‑1 (MQ=255)                               ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTGCCGCCGGCGGAAAATGGGCAGAAAGCGCCGCATCGTCTGCGTc                                                                                             >  2:1143399/1‑140 (MQ=255)                               ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTc                                                                                             <  2:1324742/140‑1 (MQ=255)                                   agagCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACa                                                                                         >  1:429520/1‑140 (MQ=255)                                         ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcaca                                                                                   >  2:667626/1‑140 (MQ=255)                                          cTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAAACGGCAGAAAGCGCCGCATCGACTGCTTCAACAGCCACaa                                                                                  >  2:487039/1‑140 (MQ=255)                                                 gCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                                           >  2:1328967/1‑140 (MQ=255)                                                       ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAGGGGCCGGGCGGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  2:1457156/140‑1 (MQ=255)                                                       ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  1:1304222/140‑1 (MQ=255)                                                       ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  2:1200526/140‑1 (MQ=255)                                                       ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCACAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGTGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  1:1143399/140‑1 (MQ=255)                                                            aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCTGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGAAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  1:206123/140‑1 (MQ=255)                                                            aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1600226/140‑1 (MQ=255)                                                            aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  1:1328967/140‑1 (MQ=255)                                                            aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1489618/140‑1 (MQ=255)                                                            aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1356753/140‑1 (MQ=255)                                                                 cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  2:1265408/140‑1 (MQ=255)                                                                 cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  2:1375069/140‑1 (MQ=255)                                                                 cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  1:1505413/140‑1 (MQ=255)                                                                 cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  1:551912/140‑1 (MQ=255)                                                                    cGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAg                                                        >  1:917897/1‑140 (MQ=255)                                                                      gCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGc                                                      <  1:987953/140‑1 (MQ=255)                                                                      gCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGc                                                      <  2:985204/140‑1 (MQ=255)                                                                       cTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGcc                                                     >  1:414836/1‑140 (MQ=255)                                                                        ggCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCCCAGCCAGTGCTACCGCCGCGGGAAAATCGGCAGAAAGCCCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCa                                                    <  2:1621071/139‑1 (MQ=255)                                                                             cgtcTGCCAGTGCAGCGTCAACAAGTGCGGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCgca                                               <  2:1452001/140‑1 (MQ=255)                                                                                   ccAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCg                                         >  1:1175831/1‑140 (MQ=255)                                                                                      gTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAgg                                      <  1:1583680/140‑1 (MQ=255)                                                                                           gCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGc                                 >  1:1426020/1‑140 (MQ=255)                                                                                              tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  1:1545003/1‑140 (MQ=255)                                                                                              tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  2:736474/1‑140 (MQ=255)                                                                                              tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  2:780449/1‑140 (MQ=255)                                                                                               caacaaGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTcc                             <  2:1403596/140‑1 (MQ=255)                                                                                                  caaGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCa                          <  1:264359/140‑1 (MQ=255)                                                                                                       gCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGaa                     <  1:424683/140‑1 (MQ=255)                                                                                                                          gTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAAcgcg  >  1:1626130/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                        ACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAACGCG  >  NC_000913/1429883‑1430143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑