Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,537,596	T→C	T420A (ACC→GCC)	narY ←	nitrate reductase Z subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,537,596	0	T	C	100.0%	273.5 / NA	72	T420A (ACC→GCC)	narY	nitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

ATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCACGC  >  NC_000913/1537460‑1537730                                                                                                                                         |                                                                                                                                       aTTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtg                                                                                                                                     <  2:957340/140‑1 (MQ=255) aTTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtg                                                                                                                                     <  2:697140/140‑1 (MQ=255) aTTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtg                                                                                                                                     <  2:717422/140‑1 (MQ=255)   ttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  1:863963/140‑1 (MQ=255)   ttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  1:1021094/140‑1 (MQ=255)   ttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  2:568823/140‑1 (MQ=255)   ttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  1:1154822/140‑1 (MQ=255)   ttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  1:1542381/140‑1 (MQ=255)   ctCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTtgtg                                                                                                                                   <  1:1462138/139‑1 (MQ=255)    tCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGa                                                                                                                                  <  2:1286961/140‑1 (MQ=255)    tCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGa                                                                                                                                  <  2:691673/140‑1 (MQ=255)    tCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGa                                                                                                                                  <  1:1087258/140‑1 (MQ=255)        gATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGc                                                                                                                              <  1:730360/140‑1 (MQ=255)        gATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCACTAACGCCTTCCACGGCTTGTGAACGc                                                                                                                              <  2:1368769/140‑1 (MQ=255)         aTGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCa                                                                                                                             <  1:1514935/140‑1 (MQ=255)                     gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCa                                                                                                                 <  2:887751/140‑1 (MQ=255)                     gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCa                                                                                                                 <  1:265835/140‑1 (MQ=255)                          cAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGcc                                                                                                            >  1:792639/1‑140 (MQ=255)                               cGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCcatcat                                                                                                       >  2:895008/1‑140 (MQ=255)                                    ttCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGtt                                                                                                  >  2:773836/1‑140 (MQ=255)                                         aGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAgc                                                                                             <  2:1276825/140‑1 (MQ=255)                                            tGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGcc                                                                                          <  2:1429066/140‑1 (MQ=255)                                             ggCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc                                                                                         >  1:1100791/1‑140 (MQ=255)                                             ggCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc                                                                                         >  1:873345/1‑140 (MQ=255)                                             ggCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc                                                                                         >  1:528181/1‑140 (MQ=255)                                             ggCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc                                                                                         >  1:561171/1‑140 (MQ=255)                                              gCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCg                                                                                        <  2:1389176/140‑1 (MQ=255)                                              gCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCg                                                                                        <  1:988856/140‑1 (MQ=255)                                                  tGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGt                                                                                    >  1:1455534/1‑140 (MQ=255)                                                    gCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTac                                                                                  <  2:1619668/140‑1 (MQ=255)                                                     cGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTacc                                                                                 >  2:191039/1‑140 (MQ=255)                                                       agaTAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccgg                                                                               <  1:41607/140‑1 (MQ=255)                                                        gaTAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccgga                                                                              >  1:423726/1‑140 (MQ=255)                                                          tAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccggacc                                                                            <  1:488863/140‑1 (MQ=255)                                                                 aCATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCg                                                                     >  2:999730/1‑140 (MQ=255)                                                                  cATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGc                                                                    >  1:66117/1‑140 (MQ=255)                                                                      tctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGc                                                                <  2:407660/140‑1 (MQ=255)                                                                       ctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCa                                                               >  1:264944/1‑140 (MQ=255)                                                                       ctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCa                                                               <  1:1531369/140‑1 (MQ=255)                                                                            aCCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCaa                                                          >  2:135802/1‑140 (MQ=255)                                                                               tGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACat                                                       >  1:995566/1‑140 (MQ=255)                                                                                gggCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACata                                                      >  1:247832/1‑140 (MQ=255)                                                                                gggCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACata                                                      >  1:155372/1‑140 (MQ=255)                                                                                gggCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACata                                                      >  1:870666/1‑140 (MQ=255)                                                                                      cGCTCAGGCCTACTTCGTCGATGGCACGAGTGTCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCg                                                >  2:1023526/1‑140 (MQ=255)                                                                                         tCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCgaga                                             <  1:895008/140‑1 (MQ=255)                                                                                         tCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCgaga                                             <  2:682985/140‑1 (MQ=255)                                                                                           aGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGata                                           <  2:423726/140‑1 (MQ=255)                                                                                            ggCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGatat                                          >  1:1612878/1‑140 (MQ=255)                                                                                                tACTTCGTCGATGGCACGAGTATCAGTAGCGCCTTCCGCGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTGCCGGACGGGTAGCGCGGGCGCTCAACATATTGGCGAGATATTGCa                                      <  2:1412066/140‑1 (MQ=255)                                                                                                tACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCa                                      >  2:1400753/1‑140 (MQ=255)                                                                                                tACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCa                                      <  1:1508209/140‑1 (MQ=255)                                                                                                tACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCa                                      <  1:773836/140‑1 (MQ=255)                                                                                                tACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCa                                      <  2:792639/140‑1 (MQ=255)                                                                                                 aCTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCAc                                     <  2:383782/140‑1 (MQ=255)                                                                                                 aCTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCACAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCAc                                     >  1:268381/1‑140 (MQ=255)                                                                                                    tcgtcgATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCgg                                  <  1:1578931/140‑1 (MQ=255)                                                                                                     cgtcgATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGa                                 >  2:687524/1‑140 (MQ=255)                                                                                                       tcgATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAAt                               >  2:29312/1‑140 (MQ=255)                                                                                                       tcgATGGCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAAt                               >  2:1502507/1‑140 (MQ=255)                                                                                                            ggCACGAGTATCAGTAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCa                          >  1:197116/1‑140 (MQ=255)                                                                                                                          tAACGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACACAGGCTTTCGATGGCg            <  1:1048941/140‑1 (MQ=255)                                                                                                                            aCGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCggg          <  2:873345/140‑1 (MQ=255)                                                                                                                             cGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgc         >  1:475773/1‑140 (MQ=255)                                                                                                                             cGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgc         >  2:8979/1‑140 (MQ=255)                                                                                                                             cGCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgc         >  1:1202659/1‑140 (MQ=255)                                                                                                                              gCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgca        >  2:72501/1‑140 (MQ=255)                                                                                                                              gCCTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgca        >  2:328960/1‑140 (MQ=255)                                                                                                                                cTTCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcagc      >  2:677383/1‑140 (MQ=255)                                                                                                                                 ttCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcagca     <  2:894054/140‑1 (MQ=255)                                                                                                                                  tCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCAc    >  2:1608362/1‑140 (MQ=255)                                                                                                                                  tCCACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCAc    >  2:1142925/1‑140 (MQ=255)                                                                                                                                    cACGGCTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCACGc  >  1:224853/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCACGC  >  NC_000913/1537460‑1537730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑