Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,570,443	A→G	L16P (CTT→CCT)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,570,443	0	A	G	100.0%	325.6 / NA	85	L16P (CTT→CCT)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/51);  total (34/51)

CACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAA  >  NC_000913/1570309‑1570573                                                                                                                                       |                                                                                                                                   cacaAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAAt                                                                                                                               >  1:18107/1‑140 (MQ=255)         cccACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCGTCGATGCCG‑TATGGCAAAGAATCCAGGTAATGTGAgctgc                                                                                                                      <  2:1286171/140‑1 (MQ=255)          ccACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAgctgc                                                                                                                      <  1:1571886/140‑1 (MQ=255)           cACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAgctgct                                                                                                                     <  2:1141056/140‑1 (MQ=255)           cACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAgctgct                                                                                                                     >  1:1319430/1‑140 (MQ=255)            aCGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCtt                                                                                                                    <  1:695266/140‑1 (MQ=255)            aCGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCtt                                                                                                                    <  2:74571/140‑1 (MQ=255)            aCGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATAAAAAGCCCGATGTTGGGAAGGTAGGGTACTCATAATCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCtt                                                                                                                    <  2:432384/140‑1 (MQ=255)              gggAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAg                                                                                                                  <  2:501612/140‑1 (MQ=255)              gggAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAg                                                                                                                  <  2:1305662/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  2:826349/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  2:1235269/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  2:550289/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  2:73143/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  1:774176/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  1:1158368/140‑1 (MQ=255)                   tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTa                                                                                                             <  2:1569278/140‑1 (MQ=255)                    aaaCCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTAc                                                                                                            <  1:955910/140‑1 (MQ=255)                       ccATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACctg                                                                                                         <  1:1025039/140‑1 (MQ=255)                        cATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACctgt                                                                                                        <  1:1186639/140‑1 (MQ=255)                        cATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACctgt                                                                                                        <  1:1487905/140‑1 (MQ=255)                          tAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACctgtct                                                                                                      >  2:1412631/1‑140 (MQ=255)                           aaaaTCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACctgtctg                                                                                                     >  2:557653/1‑140 (MQ=255)                             aaTCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTa                                                                                                   <  2:1418374/140‑1 (MQ=255)                             aaTCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTa                                                                                                   <  1:122753/140‑1 (MQ=255)                             aaTCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTa                                                                                                   <  1:577025/140‑1 (MQ=255)                               tCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACt                                                                                                 >  2:643461/1‑140 (MQ=255)                                  cGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGAt                                                                                              >  1:594190/1‑140 (MQ=255)                                  cGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGAt                                                                                              <  1:848870/140‑1 (MQ=255)                                   gCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATg                                                                                             <  2:355790/140‑1 (MQ=255)                                      tAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAg                                                                                          <  2:266197/140‑1 (MQ=255)                                      tAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTGGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAg                                                                                          <  2:666496/140‑1 (MQ=255)                                        aTAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGcc                                                                                        <  1:111323/140‑1 (MQ=255)                                        aTAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGGATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGcc                                                                                        >  1:1505559/1‑140 (MQ=255)                                          aGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCat                                                                                      <  1:458004/140‑1 (MQ=255)                                             aGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtatt                                                                                   <  2:1531052/140‑1 (MQ=255)                                               gaagaaGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtattat                                                                                 <  2:301311/140‑1 (MQ=255)                                               gaagaaGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtattat                                                                                 >  2:1359714/1‑140 (MQ=255)                                               gaagaaGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtattat                                                                                 <  1:1497579/140‑1 (MQ=255)                                               gaagaaGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtattat                                                                                 >  1:1159357/1‑140 (MQ=255)                                                 agaagaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATcc                                                                               <  2:1181506/140‑1 (MQ=255)                                                  gaagaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATccc                                                                              >  1:909299/1‑140 (MQ=255)                                                       cTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTaa                                                                         <  1:557653/140‑1 (MQ=255)                                                         aaTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTaaaa                                                                       <  1:244388/140‑1 (MQ=255)                                                          aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAAc                                                                      >  1:1540562/1‑140 (MQ=255)                                                           tGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACg                                                                     <  2:18107/140‑1 (MQ=255)                                                                aGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTAtt                                                                >  2:578382/1‑140 (MQ=255)                                                                      aTGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTc                                                          >  1:554975/1‑140 (MQ=255)                                                                            cGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAcc                                                    >  2:1019708/1‑140 (MQ=255)                                                                                ggTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAc                                                <  2:947087/140‑1 (MQ=255)                                                                                ggTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAc                                                <  2:282287/140‑1 (MQ=255)                                                                                ggTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAc                                                <  1:992992/140‑1 (MQ=255)                                                                                 gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc                                               >  1:107047/1‑140 (MQ=255)                                                                                 gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCGTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc                                               >  1:807970/1‑140 (MQ=255)                                                                                   aGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTg                                             <  2:1540562/140‑1 (MQ=255)                                                                                     ggTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtt                                           <  1:1522724/140‑1 (MQ=255)                                                                                     ggTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtt                                           <  2:1505559/140‑1 (MQ=255)                                                                                         ttCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGt                                       >  2:1174562/1‑140 (MQ=255)                                                                                            aTAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt                                    <  2:114453/140‑1 (MQ=255)                                                                                            aTAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt                                    <  2:1319430/140‑1 (MQ=255)                                                                                            aTAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt                                    <  1:28598/140‑1 (MQ=255)                                                                                               aaCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGaa                                 >  1:1428315/1‑140 (MQ=255)                                                                                                 cAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGc                               >  1:987557/1‑140 (MQ=255)                                                                                                   gCCATTACCGTCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt                             >  2:473009/1‑140 (MQ=255)                                                                                                   gCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt                             >  1:1068146/1‑140 (MQ=255)                                                                                                   gCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt                             >  2:290548/1‑140 (MQ=255)                                                                                                   gCCATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt                             >  2:812754/1‑140 (MQ=255)                                                                                                    ccATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                            >  2:1461376/1‑140 (MQ=255)                                                                                                    ccATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                            >  1:1387056/1‑140 (MQ=255)                                                                                                    ccATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                            >  1:1491130/1‑140 (MQ=255)                                                                                                    ccATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                            >  1:1535765/1‑140 (MQ=255)                                                                                                    ccATTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                            >  2:314732/1‑140 (MQ=255)                                                                                                      aTTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt                          <  2:512127/140‑1 (MQ=255)                                                                                                      aTTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt                          <  2:386245/140‑1 (MQ=255)                                                                                                      aTTACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt                          <  2:1159357/140‑1 (MQ=255)                                                                                                        tACCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCa                        >  2:1003803/1‑140 (MQ=255)                                                                                                              cGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACaa                  >  1:650950/1‑140 (MQ=255)                                                                                                              cGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACaa                  >  2:726256/1‑140 (MQ=255)                                                                                                              cGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACaa                  >  2:1315259/1‑140 (MQ=255)                                                                                                                 tGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAatat               >  2:811307/1‑140 (MQ=255)                                                                                                                     gTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTc           <  2:1495512/140‑1 (MQ=255)                                                                                                                      ttATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTcc          >  1:840215/1‑140 (MQ=255)                                                                                                                       tATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCg         <  1:314732/140‑1 (MQ=255)                                                                                                                       tATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCg         <  1:1477735/140‑1 (MQ=255)                                                                                                                              aaGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTaa  <  2:554975/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   CACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAA  >  NC_000913/1570309‑1570573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑