BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,377	A→G	Y284C (TAC→TGC)	frsA →	fermentation‑respiration switch protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,377	0	A	G	100.0%	437.5 / NA	114	Y284C (TAC→TGC)	frsA	fermentation‑respiration switch protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (57/57); total (57/57)

Only 100 of 116 total aligned reads displayed.

TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT  >  NC_000913/257241‑257513

tGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCt                                                                                                                                       >  1:1513760/1‑140 (MQ=255)

        cACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATcgc                                                                                                                               >  2:338440/1‑140 (MQ=255)

         aCCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATcgcc                                                                                                                              <  2:292618/140‑1 (MQ=255)

         aCCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATcgcc                                                                                                                              <  1:827778/140‑1 (MQ=255)

         aCCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATcgcc                                                                                                                              <  1:1134669/140‑1 (MQ=255)

          cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATcgccg                                                                                                                             <  2:652409/140‑1 (MQ=255)

                 cTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGa                                                                                                                      >  2:44545/1‑140 (MQ=255)

                 cTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGa                                                                                                                      <  1:1454231/140‑1 (MQ=255)

                  tCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGaa                                                                                                                     >  2:1437523/1‑140 (MQ=255)

                   ccAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGaaa                                                                                                                    >  2:811934/1‑140 (MQ=255)

                   ccAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGCGGATCACACTCGCGTCGCGGCCTTTGGATTCCGTTTCGGCGCTAACGTTGCCGCGCGTCTGGCATGCCTTGAATCGCCGCGTCTGaaa                                                                                                                    >  2:52745/1‑140 (MQ=255)

                    cAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAg                                                                                                                   >  2:314470/1‑140 (MQ=255)

                    cAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAg                                                                                                                   >  2:509094/1‑140 (MQ=255)

                     aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  1:1010079/1‑140 (MQ=255)

                     aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  2:1342502/1‑140 (MQ=255)

                     aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  1:802808/1‑140 (MQ=255)

                     aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  1:1308555/1‑140 (MQ=255)

                      gCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                 <  1:972917/140‑1 (MQ=255)

                      gCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                 <  2:24650/140‑1 (MQ=255)

                           ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                            <  1:1287049/140‑1 (MQ=255)

                           ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                            <  2:1206515/140‑1 (MQ=255)

                           ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                            <  1:1643136/140‑1 (MQ=255)

                           ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                            >  1:514159/1‑140 (MQ=255)

                              cATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTg                                                                                                         >  2:1409482/1‑140 (MQ=255)

                               aTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCCCGCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGt                                                                                                        <  2:244418/140‑1 (MQ=255)

                                   gCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                    >  1:1143160/1‑140 (MQ=255)

                                   gCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                    >  1:34704/1‑140 (MQ=255)

                                      cGTCTTTAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTc                                                                                                 >  2:303548/1‑140 (MQ=255)

                                      cGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTc                                                                                                 >  1:396530/1‑140 (MQ=255)

                                      cGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTc                                                                                                 <  1:1209141/140‑1 (MQ=255)

                                         cTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                              >  2:919878/1‑140 (MQ=255)

                                         cTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                              <  1:1112961/140‑1 (MQ=255)

                                         cTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                              >  2:520970/1‑140 (MQ=255)

                                          ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                             <  2:1195188/140‑1 (MQ=255)

                                          ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                             <  1:1643250/140‑1 (MQ=255)

                                          ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                             <  1:1230343/140‑1 (MQ=255)

                                          ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                             <  1:628881/140‑1 (MQ=255)

                                             aaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGt                                                                                          <  2:1466072/140‑1 (MQ=255)

                                                  gcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATa                                                                                     <  1:921821/140‑1 (MQ=255)

                                                   cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAc                                                                                    >  2:376481/1‑140 (MQ=255)

                                                   cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAc                                                                                    >  2:592686/1‑140 (MQ=255)

                                                   cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAc                                                                                    >  2:246329/1‑140 (MQ=255)

                                                   cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAc                                                                                    >  2:684439/1‑140 (MQ=255)

                                                    tGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAcc                                                                                   <  1:71261/140‑1 (MQ=255)

                                                           cGTACCGTGGGTGGATCACCCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                            <  1:590078/140‑1 (MQ=255)

                                                           cGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                            <  2:563842/140‑1 (MQ=255)

                                                           cGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                            <  2:791825/140‑1 (MQ=255)

                                                            gTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAg                                                                           <  2:1231201/140‑1 (MQ=255)

                                                            gTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAg                                                                           <  2:338336/140‑1 (MQ=255)

                                                             tACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGt                                                                          <  1:311939/140‑1 (MQ=255)

                                                              aCCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                         >  1:388708/1‑140 (MQ=255)

                                                                cGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAt                                                                       >  1:872496/1‑140 (MQ=255)

                                                                cGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAt                                                                       >  1:127859/1‑140 (MQ=255)

                                                                   gggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtttt                                                                    <  2:1480416/140‑1 (MQ=255)

                                                                    ggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTa                                                                   >  1:603435/1‑140 (MQ=255)

                                                                    ggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTa                                                                   >  1:1430371/1‑140 (MQ=255)

                                                                    ggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTa                                                                   >  1:172639/1‑140 (MQ=255)

                                                                       ggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGt                                                                <  2:1048689/140‑1 (MQ=255)

                                                                       ggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGt                                                                <  1:1409482/140‑1 (MQ=255)

                                                                        gATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTg                                                               >  2:25655/1‑140 (MQ=255)

                                                                        gATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTg                                                               <  1:44545/140‑1 (MQ=255)

                                                                         aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGc                                                              >  2:532041/1‑140 (MQ=255)

                                                                         aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGc                                                              >  2:246556/1‑140 (MQ=255)

                                                                             cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGc                                                          >  1:562105/1‑140 (MQ=255)

                                                                             cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGAAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGc                                                          >  1:1107224/1‑140 (MQ=255)

                                                                                tcgcgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAc                                                       >  1:1106345/1‑140 (MQ=255)

                                                                                 cgcgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACa                                                      <  2:34704/140‑1 (MQ=255)

                                                                                   cgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                    >  2:820473/1‑140 (MQ=255)

                                                                                   cgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                    >  1:90805/1‑140 (MQ=255)

                                                                                     tcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                  >  1:394622/1‑140 (MQ=255)

                                                                                       gcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGcc                                                >  2:889459/1‑140 (MQ=255)

                                                                                        cgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                               <  1:782039/140‑1 (MQ=255)

                                                                                           ccTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGaa                                            <  1:1437523/140‑1 (MQ=255)

                                                                                             tttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                          >  1:1284980/1‑140 (MQ=255)

                                                                                             tttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                          <  1:270361/140‑1 (MQ=255)

                                                                                              ttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATg                                         >  1:377744/1‑140 (MQ=255)

                                                                                              ttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATg                                         >  2:85497/1‑140 (MQ=255)

                                                                                                ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTa                                       <  1:246329/140‑1 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  1:1382835/140‑1 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  1:246556/140‑1 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  2:724835/140‑1 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  1:314470/140‑1 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  >  1:342151/1‑140 (MQ=255)

                                                                                                     ccGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  2:1197560/140‑1 (MQ=255)

                                                                                                        tttCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACg                               >  1:270695/1‑140 (MQ=255)

                                                                                                          tCGGCGCTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGtt                             <  1:208461/140‑1 (MQ=255)

                                                                                                               gcTAACGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGc                        <  2:603435/140‑1 (MQ=255)

                                                                                                                  aaCGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAg                     <  1:1643644/140‑1 (MQ=255)

                                                                                                                  aaCGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAg                     <  2:1010079/140‑1 (MQ=255)

                                                                                                                   aCGTTGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGt                    <  1:891157/140‑1 (MQ=255)

                                                                                                                      ttGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                 >  2:1186877/1‑140 (MQ=255)

                                                                                                                       tGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGtt                >  1:1134201/1‑140 (MQ=255)

                                                                                                                       tGCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGtt                <  1:820473/140‑1 (MQ=255)

                                                                                                                        gCCGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt               >  2:410914/1‑140 (MQ=255)

                                                                                                                          cGTGCGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgg             <  1:25655/140‑1 (MQ=255)

                                                                                                                              cGTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAt         >  2:1576726/1‑140 (MQ=255)

                                                                                                                               gTCTGGCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATg        >  2:1500541/1‑140 (MQ=255)

                                                                                                                                   ggCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatg    <  1:1641790/140‑1 (MQ=255)

                                                                                                                                   ggCATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatg    <  2:270695/140‑1 (MQ=255)

                                                                                                                                     cATGCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatgat  <  1:811934/140‑1 (MQ=255)

TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT  >  NC_000913/257241‑257513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`