Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,679	T→G	I581S (ATC→AGC)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,679	0	T	G	100.0%	285.3 / NA	77	I581S (ATC→AGC)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/47);  total (30/47)

CTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAA  >  NC_000913/2046542‑2046814                                                                                                                                          |                                                                                                                                        ctgggTATCGTTAAGTCCCAAAACATTAAGCGGGGTTCCCCATTCACCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCa                                                                                                                                       <  1:792746/137‑1 (MQ=255)       aTCGTTAAGTCCCAAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCttt                                                                                                                                 <  1:1130510/140‑1 (MQ=255)           ttAAGTACCCAAACATTGAACGCGGATTCCCATTCAACTGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGa                                                                                                                             >  2:593604/1‑140 (MQ=255)           ttAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGa                                                                                                                             >  1:636880/1‑140 (MQ=255)           ttAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGa                                                                                                                             >  1:1199524/1‑140 (MQ=255)              aGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTg                                                                                                                          >  1:547923/1‑140 (MQ=255)               gtccccAAACATTGACCGCGGATCCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTgg                                                                                                                         <  1:316784/137‑1 (MQ=255)               gTACCAAAACATTGAGCGGGGATCCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTgg                                                                                                                         <  1:1070963/140‑1 (MQ=255)                tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGa                                                                                                                        >  1:962453/1‑140 (MQ=255)                tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGa                                                                                                                        >  1:979005/1‑140 (MQ=255)                tACCAAAACTTTAAGCGCGGTTTCCCATAAAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGa                                                                                                                        <  2:1519400/140‑1 (MQ=255)                   ccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAg                                                                                                                     >  1:1609210/1‑140 (MQ=255)                   ccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAg                                                                                                                     >  1:669713/1‑140 (MQ=255)                   ccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAg                                                                                                                     >  2:149200/1‑140 (MQ=255)                    cAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGa                                                                                                                    >  1:1121219/1‑140 (MQ=255)                     aaaCATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGAt                                                                                                                   >  1:1395932/1‑140 (MQ=255)                      aaCATTGAACGCGGATCCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATa                                                                                                                  <  2:145470/140‑1 (MQ=255)                          ttGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATgg                                                                                                              >  2:1153884/1‑140 (MQ=255)                             aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGa                                                                                                           >  2:360918/1‑140 (MQ=255)                              ccgcgGTTTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGAc                                                                                                          <  1:1340587/139‑1 (MQ=255)                               cgcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACg                                                                                                         >  1:1103154/1‑140 (MQ=255)                               cgcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACg                                                                                                         >  1:1222459/1‑140 (MQ=255)                                ggggATCCCCTTTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                                                        <  2:436054/138‑1 (MQ=255)                                gcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                                                        <  1:1561590/140‑1 (MQ=255)                                gcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                                                        <  1:112885/140‑1 (MQ=255)                                gcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                                                        <  1:1128709/140‑1 (MQ=255)                                gcgGATCCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                                                        <  1:487007/140‑1 (MQ=255)                                   gATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAg                                                                                                     >  1:1373427/1‑140 (MQ=255)                                     ttCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCt                                                                                                   <  2:1477376/140‑1 (MQ=255)                                      tCCCTTTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCta                                                                                                  <  1:151835/140‑1 (MQ=255)                                      tCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCta                                                                                                  <  2:547102/140‑1 (MQ=255)                                      ccccTTTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCta                                                                                                  <  2:521518/139‑1 (MQ=255)                                       cccATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCtat                                                                                                 >  2:1618444/1‑140 (MQ=255)                                          aTTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATAcc                                                                                              <  1:1123257/140‑1 (MQ=255)                                           ttCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATAccc                                                                                             <  2:1554721/140‑1 (MQ=255)                                           ttCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATAccc                                                                                             <  1:800558/140‑1 (MQ=255)                                            tCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCa                                                                                            >  2:1016985/1‑140 (MQ=255)                                                ccGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATc                                                                                        >  1:967615/1‑140 (MQ=255)                                                ccGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATc                                                                                        <  2:471281/140‑1 (MQ=255)                                                  gCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCt                                                                                      <  1:593604/140‑1 (MQ=255)                                                     acacTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGAc                                                                                   <  2:1609210/140‑1 (MQ=255)                                                             ttttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAg                                                                           <  2:1131724/140‑1 (MQ=255)                                                             ttttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAg                                                                           <  2:1143131/140‑1 (MQ=255)                                                               ttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCGCCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCGCAGGAGCg                                                                         <  2:1402532/140‑1 (MQ=255)                                                               ttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCg                                                                         <  2:618318/140‑1 (MQ=255)                                                               ttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCg                                                                         <  1:463425/140‑1 (MQ=255)                                                               ttATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCg                                                                         <  2:636880/140‑1 (MQ=255)                                                                     cgcATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCt                                                                   >  2:1394673/1‑140 (MQ=255)                                                                            tgCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCacgc                                                            >  2:452504/1‑140 (MQ=255)                                                                                      aaTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGAt                                                  >  2:1534458/1‑140 (MQ=255)                                                                                      aaTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGAt                                                  >  1:287047/1‑140 (MQ=255)                                                                                      aaTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGAt                                                  >  1:262504/1‑140 (MQ=255)                                                                                       aTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATg                                                 >  1:1479083/1‑140 (MQ=255)                                                                                       aTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGGGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATg                                                 >  2:1387208/1‑140 (MQ=255)                                                                                        tCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGc                                                <  2:463269/140‑1 (MQ=255)                                                                                        tCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGc                                                <  2:872390/140‑1 (MQ=255)                                                                                        tCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGc                                                <  1:1153884/140‑1 (MQ=255)                                                                                          cTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCca                                              <  2:1374626/140‑1 (MQ=255)                                                                                            gttgtCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCcaca                                            <  2:962453/140‑1 (MQ=255)                                                                                             ttgtCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAg                                           >  1:854472/1‑140 (MQ=255)                                                                                             ttgtCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAg                                           <  2:1103154/140‑1 (MQ=255)                                                                                             ttgtCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAg                                           <  2:1222459/140‑1 (MQ=255)                                                                                              tgtCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGc                                          >  2:1644302/1‑140 (MQ=255)                                                                                                  gggCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGaa                                      <  2:208433/140‑1 (MQ=255)                                                                                                  gggCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGaa                                      <  1:149200/140‑1 (MQ=255)                                                                                                     cTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATgg                                   >  1:645614/1‑140 (MQ=255)                                                                                                       ggTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGTAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGgtg                                 >  1:561306/1‑140 (MQ=255)                                                                                                       ggTGCTCTCGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGgtg                                 <  2:979005/140‑1 (MQ=255)                                                                                                            tctcGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGAt                            <  2:1373427/140‑1 (MQ=255)                                                                                                             ctcGACGCGTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                           <  1:360918/140‑1 (MQ=255)                                                                                                                   gcgTCACGAAGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTa                     <  2:272689/140‑1 (MQ=255)                                                                                                                         ggAAGGTGTTCAGGACAGCCCCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGccc               <  2:13241/139‑1 (MQ=255)                                                                                                                          gagggTGTTAAGAACAGCACCCTTAGTGACTGGAAAGATAAGGGGGACGGAACCTATACCCAAATCCTACCCAAAGGACCTATGTTGGGCCCGAAGCCGATGATGCCACAGCTGAATGGTGTGGTTCCGGTTAAGGcccc              <  2:669713/137‑1 (MQ=255)                                                                                                                            aGGTGTTCAGGACAGCCCCTTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCccg            <  1:114918/140‑1 (MQ=255)                                                                                                                            aGGTGTTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGGGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCccg            <  2:1395932/140‑1 (MQ=255)                                                                                                                              gtgtTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATCCCCAGATCCTGCCCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCccgcc          <  2:1036244/140‑1 (MQ=255)                                                                                                                                gtTCAGGACAGCACCCTTTCTGACTGGAAAGATAATGGGGCCGGAAGCTATACCCAGTTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCgt        <  1:591068/140‑1 (MQ=255)                                                                                                                                    aGGACAGCCCCCTTTCTGACGGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCgtggtg    <  2:1199524/140‑1 (MQ=255)                                                                                                                                      gACAGCCCCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTGTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGaa  <  1:691067/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAA  >  NC_000913/2046542‑2046814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑