Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,696,623	G→A	V275I (GTC→ATC)	mltF →	membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,696,623	0	G	A	100.0%	337.2 / NA	96	V275I (GTC→ATC)	mltF	membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (60/36);  total (60/36)

ACTTGGTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACA  >  NC_000913/2696488‑2696759                                                                                                                                        |                                                                                                                                          aCTTGGTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGa                                                                                                                                       >  1:352045/1‑140 (MQ=255)  cTTGGTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGAt                                                                                                                                      >  2:1023204/1‑140 (MQ=255)   ttGGTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATa                                                                                                                                     >  2:826275/1‑140 (MQ=255)   ttGGTTTAGCCCGTTAGATGGCGATAAAACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATa                                                                                                                                     <  2:134884/140‑1 (MQ=255)     ggTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATAcg                                                                                                                                   >  2:319616/1‑140 (MQ=255)         tAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGca                                                                                                                               <  1:300012/140‑1 (MQ=255)         tAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGca                                                                                                                               >  1:928686/1‑140 (MQ=255)                ttAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑ttttt                                                                                                                       >  1:12056/1‑140 (MQ=255)                ttAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑ttttt                                                                                                                       >  2:301829/1‑140 (MQ=255)                   gATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTAcg                                                                                                                    <  2:1466643/140‑1 (MQ=255)                    aTGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTAcgc                                                                                                                   <  2:1388722/140‑1 (MQ=255)                      ggCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACgcgc                                                                                                                 >  2:842025/1‑140 (MQ=255)                      ggCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACgcgc                                                                                                                 >  2:83129/1‑140 (MQ=255)                      ggCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACgcgc                                                                                                                 >  1:541724/1‑140 (MQ=255)                       gCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGcc                                                                                                                <  1:855632/140‑1 (MQ=255)                             ataCCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGAt                                                                                                          >  2:869674/1‑140 (MQ=255)                              taCCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATg                                                                                                         <  1:1417487/140‑1 (MQ=255)                               aCCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGc                                                                                                        >  1:1156870/1‑140 (MQ=255)                                 ccTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCgg                                                                                                      >  1:857186/1‑140 (MQ=255)                                   tttCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCCCGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTa                                                                                                    >  1:1223623/1‑140 (MQ=255)                                   tttCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTa                                                                                                    <  2:1569074/140‑1 (MQ=255)                                    ttCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTAc                                                                                                   >  1:557942/1‑140 (MQ=255)                                     tCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACt                                                                                                  >  2:145018/1‑140 (MQ=255)                                     tCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACt                                                                                                  >  2:733449/1‑140 (MQ=255)                                      ccgccgCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTg                                                                                                 >  2:1603369/1‑140 (MQ=255)                                         ccgccCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCg                                                                                              >  1:1092244/1‑140 (MQ=255)                                         ccgccCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCg                                                                                              <  1:1427127/140‑1 (MQ=255)                                           gccCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCa                                                                                            >  1:533353/1‑140 (MQ=255)                                             ccTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGt                                                                                          <  1:1578263/140‑1 (MQ=255)                                                  tCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAg                                                                                     >  1:1281241/1‑140 (MQ=255)                                                   cGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGc                                                                                    <  1:1644453/140‑1 (MQ=255)                                                   cGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGc                                                                                    <  2:494832/140‑1 (MQ=255)                                                   cGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGc                                                                                    <  1:135278/140‑1 (MQ=255)                                                    gACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcc                                                                                   >  1:361125/1‑140 (MQ=255)                                                     aCTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGccc                                                                                  <  2:352045/140‑1 (MQ=255)                                                     aCTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGccc                                                                                  <  2:716756/140‑1 (MQ=255)                                                     aCTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGccc                                                                                  <  1:209812/140‑1 (MQ=255)                                                      cttcttCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc                                                                                 <  2:497430/140‑1 (MQ=255)                                                      cttcttCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc                                                                                 >  1:540263/1‑140 (MQ=255)                                                      cttcttCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc                                                                                 >  1:931877/1‑140 (MQ=255)                                                      cttcttCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc                                                                                 <  1:1460608/140‑1 (MQ=255)                                                      cttcttCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc                                                                                 >  1:1569415/1‑140 (MQ=255)                                                           tcAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGttt                                                                            >  2:428342/1‑140 (MQ=255)                                                                gAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGaa                                                                       >  1:609441/1‑140 (MQ=255)                                                                    tgaatgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATAc                                                                   >  2:1237208/1‑140 (MQ=255)                                                                    tgaatgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATAc                                                                   >  1:517317/1‑140 (MQ=255)                                                                        tgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCg                                                               <  1:501258/140‑1 (MQ=255)                                                                        tgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCg                                                               <  2:533353/140‑1 (MQ=255)                                                                           aGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCgaag                                                            >  2:762417/1‑140 (MQ=255)                                                                              cGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGaaa                                                         >  2:25345/1‑140 (MQ=255)                                                                                gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAAtt                                                       >  1:525204/1‑140 (MQ=255)                                                                                 tACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTg                                                      >  2:970736/1‑140 (MQ=255)                                                                                 tACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTg                                                      >  2:557370/1‑140 (MQ=255)                                                                                  aCGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGa                                                     <  1:684422/140‑1 (MQ=255)                                                                                    gCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACt                                                   >  2:501281/1‑140 (MQ=255)                                                                                      tGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTgg                                                 >  2:1288896/1‑140 (MQ=255)                                                                                       ggCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGc                                                >  1:601538/1‑140 (MQ=255)                                                                                         cACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGt                                              >  1:1303441/1‑140 (MQ=255)                                                                                           cGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGTGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGttt                                            >  1:1299511/1‑140 (MQ=255)                                                                                           cGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGttt                                            >  1:595903/1‑140 (MQ=255)                                                                                           cGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGttt                                            >  2:999556/1‑140 (MQ=255)                                                                                             cATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGc                                          >  1:235144/1‑140 (MQ=255)                                                                                              aTTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCt                                         >  1:180492/1‑140 (MQ=255)                                                                                                 gAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGc                                      <  1:319616/140‑1 (MQ=255)                                                                                                  aaGAGAAATACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGcc                                     >  2:910158/1‑140 (MQ=255)                                                                                                       aaaTACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAt                                <  2:557942/140‑1 (MQ=255)                                                                                                       aaaTACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAt                                <  1:301829/140‑1 (MQ=255)                                                                                                       aaaTACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAt                                >  2:1536665/1‑140 (MQ=255)                                                                                                        aaTACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAtt                               >  2:500919/1‑140 (MQ=255)                                                                                                          tACCTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGc                             <  2:1156870/140‑1 (MQ=255)                                                                                                            ccTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCtt                           >  2:160556/1‑140 (MQ=255)                                                                                                            ccTGGGGCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCtt                           >  1:528295/1‑140 (MQ=255)                                                                                                               ggggCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                        >  1:1048504/1‑140 (MQ=255)                                                                                                               ggggCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                        >  2:1260368/1‑140 (MQ=255)                                                                                                                  gCATGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAgg                     <  1:272602/140‑1 (MQ=255)                                                                                                                    aTGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGaa                   <  2:49519/140‑1 (MQ=255)                                                                                                                     tGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                  >  2:300940/1‑140 (MQ=255)                                                                                                                     tGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                  >  1:1053008/1‑140 (MQ=255)                                                                                                                     tGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                  >  1:667847/1‑140 (MQ=255)                                                                                                                     tGGCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                  >  1:75614/1‑140 (MQ=255)                                                                                                                      ggCGATGATTTTGATTACATCGATACGCGCACATTTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                  >  2:79377/1‑140 (MQ=255)                                                                                                                      ggCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATc                 >  2:539191/1‑140 (MQ=255)                                                                                                                      ggCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATc                 >  2:561837/1‑140 (MQ=255)                                                                                                                      ggCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATc                 >  2:416079/1‑140 (MQ=255)                                                                                                                       gCGATGATTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCg                >  2:1274284/1‑140 (MQ=255)                                                                                                                         gatgatTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa              <  2:125154/140‑1 (MQ=255)                                                                                                                         gatgatTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa              <  2:525204/140‑1 (MQ=255)                                                                                                                         gatgatTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa              <  2:517317/140‑1 (MQ=255)                                                                                                                           tgatTTTGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACt            >  2:1323823/1‑140 (MQ=255)                                                                                                                              ttttGATTCCATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTggg         <  2:609441/140‑1 (MQ=255)                                                                                                                              ttttGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTggg         <  1:970736/140‑1 (MQ=255)                                                                                                                              ttttGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTggg         <  1:1603369/140‑1 (MQ=255)                                                                                                                              ttttGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTggg         <  1:1365232/140‑1 (MQ=255)                                                                                                                              ttttGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTggg         <  1:1237208/140‑1 (MQ=255)                                                                                                                                 tGATTACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATg      <  2:235144/140‑1 (MQ=255)                                                                                                                                     tACATCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGcaca  <  2:540263/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          ACTTGGTTTAGCCCGTTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACA‑TTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACA  >  NC_000913/2696488‑2696759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑