Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,496	T→C	M77T (ATG→ACG)	yggW →	putative coproporphyrinogen‑III oxidase‑like protein YggW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,496	0	T	C	100.0%	208.6 / NA	56	M77T (ATG→ACG)	yggW	putative coproporphyrinogen‑III oxidase‑like protein YggW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/48);  total (8/48)

GAGAAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCATCTC  >  NC_000913/3097361‑3097631                                                                                                                                        |                                                                                                                                        gagaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTTCGGCCCCGCGACGCaa                                                                                                                                     >  1:920425/1‑140 (MQ=255)  agaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCaaa                                                                                                                                    <  2:267153/140‑1 (MQ=255)  agaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCaaa                                                                                                                                    <  1:1219999/140‑1 (MQ=255)  agaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCaaa                                                                                                                                    <  1:164721/140‑1 (MQ=255)  agaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATATTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCaaa                                                                                                                                    <  1:619494/140‑1 (MQ=255)   gaAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTTCGGCCCGGCGACGCCAAc                                                                                                                                   >  1:199263/1‑140 (MQ=255)    aaGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACg                                                                                                                                  <  1:1367465/140‑1 (MQ=255)         ccGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGCGCCCGCTTTCCCGCCCCGCGACGCAAAcgccgcc                                                                                                                             >  2:362670/1‑139 (MQ=255)         ccGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTTCCGCCCCGCGACGCAAAAgccgcc                                                                                                                             >  1:429736/1‑139 (MQ=255)          cGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGctgctg                                                                                                                            <  1:1320605/140‑1 (MQ=255)            cACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCCAGCCCGCTTTCCCGCCCCGCGGCGCCAACGCCGCCggg                                                                                                                          >  2:710731/1‑139 (MQ=255)            cACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGCCGCCGGGCCTGCTTTCCCGCCCCGCGGCGCCAACGCTGCTggg                                                                                                                          >  2:684395/1‑139 (MQ=255)             acgacgATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGAc                                                                                                                         <  2:1000346/140‑1 (MQ=255)                     tATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGgcgtgcg                                                                                                                 <  2:638847/140‑1 (MQ=255)                       tGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGcgtgcgtg                                                                                                               <  2:349084/140‑1 (MQ=255)                           cAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGTGCCGTGAAGTAAAGACAATCTTTATTGGCGGTTGGACCCCGAGCCTGCTTTTCTGCCCGGCGGCGCGAACACTTCTGGACGGGGGGGGTGcgcg                                                                                                           >  2:704679/1‑140 (MQ=255)                              cATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTTCGGCCCCGCGGCGCAAACGCCGCTGGGCGGCGGGTGTGCGCGgtt                                                                                                        >  2:791990/1‑140 (MQ=255)                                  tGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCg                                                                                                    <  2:670639/140‑1 (MQ=255)                                          gATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGc                                                                                            <  1:1548826/140‑1 (MQ=255)                                          gATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGc                                                                                            <  2:228598/140‑1 (MQ=255)                                          gATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGc                                                                                            <  1:1287584/140‑1 (MQ=255)                                            tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCgg                                                                                          <  2:1549015/140‑1 (MQ=255)                                            tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGGCGCCGCGCCCGCCTTTCCGCCCCGCGACGCCAAAGCCGCTGGGCGGCGGGCGGGCGCGGTTGCCGCCGGCCGCgg                                                                                          >  2:709130/1‑140 (MQ=255)                                            tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTCCGGCCCCGCGACGCAAACGCCGCTGGGCGGCGGGCGGGGGCGGTTGCCGCcggcggcgg                                                                                          >  2:24007/1‑140 (MQ=255)                                            tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCCAGCCCGCCTTTCCGCCCCGCGACGCCAACGCCGCTGGGCGGCGGGCGGGCGCGGTTGCCGCCGGCCGCgg                                                                                          >  2:233296/1‑140 (MQ=255)                                              tGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGAt                                                                                        <  2:1405769/140‑1 (MQ=255)                                              tGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCCGCTTTCCGGCCCCGCGGCGCAAACGCCGCTGGACGGGGGGCGTGCGCGTTTGCCCCCGGCAGCGGag                                                                                        >  2:283060/1‑139 (MQ=255)                                              tGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCCCGCCCGCCTTTCGGCCCCGCCGCCCCAACGCCGCTGGGCGGGGGGCGGGCGCGTTTGGCGCCGGCCGCgggg                                                                                        >  2:947153/1‑138 (MQ=255)                                              tGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCCTTCCCGCCCGGCGACGCAAACGCTGCTGGGCGGCGGGCGGGCGCGTTTGCCGCCGGCCGCgggg                                                                                        >  2:769879/1‑138 (MQ=255)                                              tGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGCACCCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGGCGGCGTGCGTGCGCGTTTTCCGCTGGGAGCGGAt                                                                                        >  2:417681/1‑140 (MQ=255)                                                gACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGc                                                                                      <  1:244233/140‑1 (MQ=255)                                                     cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGCGCCTGCTTTTCCGCCCCGCGGCGCAAACCCCGCTGGGCGGCGGGCGTGCGCGTTTTCCGCCGGCCGCGGGGGCAGaaa                                                                                 >  2:189053/1‑140 (MQ=255)                                                          tgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCCAGCCCGCTTTCCCGCCCCGCGGCGCCAACGCCGCTGGGCGGCGGGCGGGCGCGGTTGCCGCCGGCCGCGGGTGGAGAAAATAcc                                                                            >  2:1116287/1‑139 (MQ=255)                                                          tgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACCCCCAGCCCGCTTTCCCGCCCCGCGACGCAAACGCCGCTGGACGGCGGGCGGGGGCGGTTGCCGCCGGCCGCGGGTGCAGGAAATAcc                                                                            >  2:228026/1‑139 (MQ=255)                                                           ggCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTa                                                                           <  1:92474/140‑1 (MQ=255)                                                                aCGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGaa                                                                      <  2:1561679/140‑1 (MQ=255)                                                                  gCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGc                                                                    <  2:1093951/140‑1 (MQ=255)                                                                      aGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTCCGGCCCCGCGACCCAAACGCTGCTGGGCGGCGTGCGGGCGCGGTTGCCGCCGGCCGCGGGTGCAGAAAATACTATGGGAGCGGAc                                                                >  2:224347/1‑140 (MQ=255)                                                                            gTGAAGTAAAGACAATCTTTATTGGCGGTGATGCGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGc                                                          <  1:1520563/140‑1 (MQ=255)                                                                             tGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCa                                                         <  1:710731/140‑1 (MQ=255)                                                                             tGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCa                                                         <  1:1168540/140‑1 (MQ=255)                                                                                       aCAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTTCGGCCCGGCGGCGCCAACGCCGCTGGGCGGCGGGCGGGCGCGTTTTCCGCCGGCCGCGGGTGGAGAAAATACTATGGAAGCGGAACCCCGCCCGGGAGAAGc                                               >  2:921715/1‑140 (MQ=255)                                                                                         aaTCTTTATTGGCGGTGGGTCGCCGAGCCTGCCTTTCGGCCCCGCGACGCAAACGCTGCTGGACGGCGTGCGCGCGCGTGTGCCGCCGGCCGCGGGTGCAGAAAATACTATGGAAGGGGACCCCGGCGCGGGAGAAGCCg                                             >  2:127828/1‑140 (MQ=255)                                                                                          aTCTTTATTGGCGGTGGTACGCCGCGCCCGCTTTTCCGCCCCGCGACGCCAAAGCTGCTGGGCGGCGGGCGTGCGCGGTTTTCGCTGGCCGCGGGGGCAGAAAATACTATGGGAGCGAACCCCGGCACGGGAGAAGGCgc                                            >  2:335601/1‑139 (MQ=255)                                                                                           tCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGAt                                           <  1:362670/140‑1 (MQ=255)                                                                                           tCTTTATTGGCGGTGGTACGCCGAGCCCGCTTTTCGGCCCCGCGGCGCAAAAGCTTCTGGACGGCGGGCGGGCGCGTTTGTCGCCGGCAGCGGGAGCAGAAAATACTATGGAAGCGAAACCCCGCCCGGTGGAAACCGag                                           >  2:904178/1‑139 (MQ=255)                                                                                                tttGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCtt                                      <  1:592966/139‑1 (MQ=255)                                                                                                aTTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCtt                                      <  1:189053/140‑1 (MQ=255)                                                                                                aTTGGCGGTGGTACGCCGAGCCCGCTTTTCGGCCCGGCGACGCAAACGCTGCTGGGCGGCGGGCGGGCGCGGTTTGCGCTGGGAGCGGGTGCAGAAAATACTATGGAAGCGAACCCCGGCACGGGAGAAGCCGAACGcct                                      >  2:547440/1‑138 (MQ=255)                                                                                                   ggCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGt                                   <  1:1634306/140‑1 (MQ=255)                                                                                                    gCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTc                                  <  1:1493969/140‑1 (MQ=255)                                                                                                     cGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCg                                 <  1:1116287/140‑1 (MQ=255)                                                                                                       gtggtACGCCGAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGAt                               <  2:920425/140‑1 (MQ=255)                                                                                                                 gAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGTGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTg                     <  2:283476/140‑1 (MQ=255)                                                                                                                 gAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTg                     <  2:199263/140‑1 (MQ=255)                                                                                                                 gAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTg                     <  1:691855/140‑1 (MQ=255)                                                                                                                 gAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTg                     <  2:1575207/140‑1 (MQ=255)                                                                                                                 gAGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTg                     >  1:299229/1‑140 (MQ=255)                                                                                                                  aGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGc                    >  2:168231/1‑140 (MQ=255)                                                                                                                  aGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGc                    <  2:938512/140‑1 (MQ=255)                                                                                                                  aGCCTGCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGc                    >  1:851720/1‑140 (MQ=255)                                                                                                                       gCTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGgtg               >  1:273716/1‑140 (MQ=255)                                                                                                                        cTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGgtgt              >  1:226546/1‑140 (MQ=255)                                                                                                                        cTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGgtgt              >  1:1151856/1‑140 (MQ=255)                                                                                                                        cTTTCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGgtgt              <  1:843213/140‑1 (MQ=255)                                                                                                                         tttCCGGCCCGGCGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGtgtg             <  1:709130/140‑1 (MQ=255)                                                                                                                            ccggcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAAc          <  1:417681/140‑1 (MQ=255)                                                                                                                            ccggcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAAc          <  2:1499590/140‑1 (MQ=255)                                                                                                                            ccggcccggcGACGCAAACACTGCTGGACGGCGTGCGTGCGTGTATGCCGCTGGCAGCGGATTCAGAAATTACTATGGAAGCGAGCCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAAc          <  2:1616363/140‑1 (MQ=255)                                                                                                                             cggcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAAcc         <  1:1004977/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  2:783486/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  1:339364/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  2:429736/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  2:1404470/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  2:335244/140‑1 (MQ=255)                                                                                                                               gcccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  1:769879/140‑1 (MQ=255)                                                                                                                               gcccggcGACGAAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGc       <  1:229802/140‑1 (MQ=255)                                                                                                                                 ccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAt     <  1:24007/140‑1 (MQ=255)                                                                                                                                 ccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAt     <  1:1388076/140‑1 (MQ=255)                                                                                                                                 ccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAt     <  2:248661/140‑1 (MQ=255)                                                                                                                                 ccggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAt     <  1:1014078/140‑1 (MQ=255)                                                                                                                                   ggcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAtct   >  1:436604/1‑140 (MQ=255)                                                                                                                                    gcGACGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCAtctc  >  2:532097/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GAGAAGTGCCGCACGACGATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTGAACCGCATCTC  >  NC_000913/3097361‑3097631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑