Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,220,362	A→G	N290S (AAC→AGC)	patA →	putrescine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,362	0	A	G	100.0%	335.1 / NA	89	N290S (AAC→AGC)	patA	putrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (64/25);  total (64/25)

GCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTT  >  NC_000913/3220227‑3220492                                                                                                                                        |                                                                                                                                   gCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTa                                                                                                                                >  2:847797/1‑140 (MQ=255) gCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTa                                                                                                                                >  2:1475771/1‑140 (MQ=255) gCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTa                                                                                                                                <  1:1384843/140‑1 (MQ=255)     aaTTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGc                                                                                                                            >  2:414279/1‑140 (MQ=255)         cTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGa                                                                                                                        <  1:1369403/140‑1 (MQ=255)              gccgccgGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATcc                                                                                                                   <  1:71063/140‑1 (MQ=255)                  ccgGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTg                                                                                                               <  1:1096760/140‑1 (MQ=255)                    gggCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGcc                                                                                                             <  1:1528681/140‑1 (MQ=255)                            tcACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCaaa                                                                                                     <  2:754439/140‑1 (MQ=255)                                cgccgTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAgcgc                                                                                                 >  1:1592113/1‑140 (MQ=255)                                    gtacgtaAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTcgg                                                                                             >  1:867618/1‑140 (MQ=255)                                     tacgtaAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTcggc                                                                                            >  1:561979/1‑140 (MQ=255)                                     tacgtaAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTcggc                                                                                            >  1:1624245/1‑140 (MQ=255)                                     tacgtaAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTcggc                                                                                            >  2:300943/1‑140 (MQ=255)                                            gCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGgcggcggcg                                                                                     >  1:251828/1‑140 (MQ=255)                                            gCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGgcggcggcg                                                                                     >  1:862843/1‑140 (MQ=255)                                            gCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGCACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGgcggcggcg                                                                                     >  2:1031539/1‑140 (MQ=255)                                               aTGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGa                                                                                  >  2:1286160/1‑140 (MQ=255)                                                tGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGac                                                                                 >  1:588614/1‑139 (MQ=255)                                                tGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGAt                                                                                 >  2:30996/1‑140 (MQ=255)                                                tGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGAt                                                                                 >  2:1615383/1‑140 (MQ=255)                                                tGCGATGAGTTCGGCACACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGAt                                                                                 >  1:1424808/1‑140 (MQ=255)                                                 gCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATg                                                                                >  1:1195109/1‑140 (MQ=255)                                                  cGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGAGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGc                                                                               >  1:591043/1‑140 (MQ=255)                                                      gAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGAt                                                                           >  2:764964/1‑140 (MQ=255)                                                      gAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGAt                                                                           >  1:1361030/1‑140 (MQ=255)                                                       aGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGAtt                                                                          >  2:110822/1‑140 (MQ=255)                                                          tCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTggg                                                                       >  2:1514222/1‑139 (MQ=255)                                                           cGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGgcg                                                                      >  1:194564/1‑140 (MQ=255)                                                           cGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGgcg                                                                      >  1:679445/1‑140 (MQ=255)                                                           cGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGgcg                                                                      <  2:606874/140‑1 (MQ=255)                                                           cGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGgcg                                                                      <  1:300943/140‑1 (MQ=255)                                                                 aCTGATGATCCTCGATGAAGTACAAACGGGCATAGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCa                                                                >  2:927078/1‑140 (MQ=255)                                                                  cTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCAt                                                               <  1:1475771/140‑1 (MQ=255)                                                                   tgatgaTCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATc                                                              >  1:808344/1‑140 (MQ=255)                                                                   tgatgaTCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATc                                                              >  2:744360/1‑140 (MQ=255)                                                                     atgatCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGc                                                            <  2:1503499/140‑1 (MQ=255)                                                                          ccTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTg                                                       >  2:1098037/1‑140 (MQ=255)                                                                          ccTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTg                                                       >  2:606802/1‑140 (MQ=255)                                                                           cTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGa                                                      >  2:719358/1‑140 (MQ=255)                                                                            tCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGaa                                                     >  2:1017894/1‑140 (MQ=255)                                                                             cGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAag                                                    >  1:583155/1‑140 (MQ=255)                                                                               aTGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAagag                                                  >  1:661388/1‑140 (MQ=255)                                                                               aTGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAagag                                                  >  2:36221/1‑140 (MQ=255)                                                                               aTGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAagag                                                  >  1:1212289/1‑140 (MQ=255)                                                                                 gAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGgt                                                <  2:862843/140‑1 (MQ=255)                                                                                   aGTACAAACGGGTATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGgtgt                                              >  1:1595574/1‑140 (MQ=255)                                                                                   aGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGgtgt                                              >  1:480263/1‑140 (MQ=255)                                                                                     tACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGttt                                            >  1:480297/1‑140 (MQ=255)                                                                                     tACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGttt                                            >  1:813935/1‑140 (MQ=255)                                                                                      aCAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGtttt                                           <  1:473496/140‑1 (MQ=255)                                                                                        aaaCGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGTCACTGAAGAGGTGTTTTCa                                         >  1:316382/1‑140 (MQ=255)                                                                                        aaaCGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCa                                         >  1:281809/1‑140 (MQ=255)                                                                                         aaCGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAg                                        <  1:163393/140‑1 (MQ=255)                                                                                          aCGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGt                                       >  1:1553026/1‑140 (MQ=255)                                                                                            gggCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACGGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTc                                     >  2:132880/1‑140 (MQ=255)                                                                                            gggCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTc                                     >  1:1023456/1‑140 (MQ=255)                                                                                             ggCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCt                                    >  1:398650/1‑140 (MQ=255)                                                                                             ggCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCt                                    >  1:212560/1‑140 (MQ=255)                                                                                             ggCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCt                                    <  1:1546615/140‑1 (MQ=255)                                                                                               cATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGt                                  >  2:593793/1‑140 (MQ=255)                                                                                               cATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGt                                  >  1:1282325/1‑140 (MQ=255)                                                                                               cATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGt                                  >  2:824585/1‑140 (MQ=255)                                                                                                    ggCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGAc                             >  1:372171/1‑140 (MQ=255)                                                                                                     gcgcACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACa                            <  1:52072/140‑1 (MQ=255)                                                                                                     gcgcACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTACTGTTCGACa                            <  1:1031539/140‑1 (MQ=255)                                                                                                        cACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAAcc                         <  2:808344/140‑1 (MQ=255)                                                                                                        cACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAAcc                         <  2:467300/140‑1 (MQ=255)                                                                                                        cACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAAcc                         >  1:1355025/1‑140 (MQ=255)                                                                                                        cACGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAAcc                         >  1:386762/1‑140 (MQ=255)                                                                                                          cGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCa                       <  2:1412814/140‑1 (MQ=255)                                                                                                          cGGGCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGACGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCa                       <  2:588614/140‑1 (MQ=255)                                                                                                           gggCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCAt                      <  2:1361030/140‑1 (MQ=255)                                                                                                             gCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTc                    >  1:1595114/1‑140 (MQ=255)                                                                                                             gCAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTc                    >  2:1360597/1‑140 (MQ=255)                                                                                                              cAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTcc                   >  2:434149/1‑140 (MQ=255)                                                                                                              cAAGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTcc                   <  1:403472/140‑1 (MQ=255)                                                                                                                aGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTg                 >  1:516349/1‑140 (MQ=255)                                                                                                                aGATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTg                 <  2:251828/140‑1 (MQ=255)                                                                                                                 gATGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGc                <  1:1286160/140‑1 (MQ=255)                                                                                                                  aTGTTCGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCa               <  2:591043/140‑1 (MQ=255)                                                                                                                       cGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATacca          >  2:498363/1‑140 (MQ=255)                                                                                                                       cGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATacca          >  1:406678/1‑140 (MQ=255)                                                                                                                       cGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATacca          >  1:452887/1‑140 (MQ=255)                                                                                                                       cGCCTGCGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATacca          >  2:245570/1‑140 (MQ=255)                                                                                                                             cGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCt    >  2:1169894/1‑140 (MQ=255)                                                                                                                             cGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCt    >  1:1337610/1‑140 (MQ=255)                                                                                                                             cGAGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCt    >  1:781386/1‑140 (MQ=255)                                                                                                                               aGCATGAGAGCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCttt  >  1:1059694/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTT  >  NC_000913/3220227‑3220492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑