Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,227	T→C	N88S (AAC→AGC)	nanR ←	DNA‑binding transcriptional dual regulator NanR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,227	0	T	C	100.0%	347.8 / NA	92	N88S (AAC→AGC)	nanR	DNA‑binding transcriptional dual regulator NanR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/44);  total (48/44)

ATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCACGACGGCGGATCAT  >  NC_000913/3374093‑3374363                                                                                                                                       |                                                                                                                                         aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     <  2:1275488/140‑1 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     >  2:534994/1‑140 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     >  2:717516/1‑140 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     >  1:893546/1‑140 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     <  2:1589743/140‑1 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     >  1:1473363/1‑140 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     <  1:1447968/140‑1 (MQ=255) aTTCAAAGAACAGACGTAATTGTTCGAAATGGGCAACCCAGCCGGGAAGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTAtt                                                                                                                                     <  2:811744/140‑1 (MQ=255)   tCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTg                                                                                                                                   >  2:1518238/1‑140 (MQ=255)     aaaGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCa                                                                                                                                 >  1:490542/1‑140 (MQ=255)        gAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCa                                                                                                                              <  1:242951/140‑1 (MQ=255)           cAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGAc                                                                                                                           >  2:1474876/1‑140 (MQ=255)              aCGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCtt                                                                                                                        >  1:1299154/1‑140 (MQ=255)                   aTTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGt                                                                                                                   >  2:463039/1‑140 (MQ=255)                        tCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAACGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTaa                                                                                                              >  2:1209162/1‑140 (MQ=255)                        tCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTaa                                                                                                              >  1:81896/1‑140 (MQ=255)                        tCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTaa                                                                                                              >  2:1576451/1‑140 (MQ=255)                        tCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTaa                                                                                                              >  2:1521291/1‑140 (MQ=255)                        tCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTaa                                                                                                              >  2:90108/1‑140 (MQ=255)                         cGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAAc                                                                                                             >  1:160265/1‑140 (MQ=255)                         cGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAAc                                                                                                             <  2:554216/140‑1 (MQ=255)                            aaTGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCt                                                                                                          <  1:756433/140‑1 (MQ=255)                              tGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGc                                                                                                        <  2:1021862/140‑1 (MQ=255)                              tGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGc                                                                                                        <  1:51203/140‑1 (MQ=255)                              tGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGc                                                                                                        <  2:99440/140‑1 (MQ=255)                               gggCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGcc                                                                                                       <  1:70885/140‑1 (MQ=255)                                  cAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAgc                                                                                                    <  2:957548/140‑1 (MQ=255)                                  cAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAgc                                                                                                    <  1:15639/140‑1 (MQ=255)                                  cAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAgc                                                                                                    <  2:83781/140‑1 (MQ=255)                                      cccACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCtt                                                                                                >  1:1617166/1‑140 (MQ=255)                                         aCCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCAc                                                                                             <  2:1520895/140‑1 (MQ=255)                                           cGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGc                                                                                           <  1:1518238/140‑1 (MQ=255)                                             ggATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGTTAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGCCCTTTGCGTTGTAACGCTGCCAGCGCTTCACGCAc                                                                                         >  2:1014197/1‑140 (MQ=255)                                               aTGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCg                                                                                       >  2:483165/1‑140 (MQ=255)                                               aTGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCg                                                                                       >  2:16541/1‑140 (MQ=255)                                                tGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGa                                                                                      >  1:1527609/1‑140 (MQ=255)                                                  agaAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAg                                                                                    >  2:413726/1‑140 (MQ=255)                                                    aaaGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGa                                                                                  >  1:1213601/1‑140 (MQ=255)                                                    aaaGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGa                                                                                  <  2:615039/140‑1 (MQ=255)                                                        gAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGcc                                                                              <  2:490542/140‑1 (MQ=255)                                                        gAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGcc                                                                              <  2:1299154/140‑1 (MQ=255)                                                        gAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGcc                                                                              <  2:474560/140‑1 (MQ=255)                                                         aaaTCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGccc                                                                             >  1:1596219/1‑140 (MQ=255)                                                            tCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGAc                                                                          >  2:178318/1‑140 (MQ=255)                                                                  gCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTaaa                                                                    >  2:372383/1‑140 (MQ=255)                                                                  gCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTaaa                                                                    >  1:2649/1‑140 (MQ=255)                                                                   ccATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAg                                                                   >  1:1559552/1‑140 (MQ=255)                                                                       gCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACg                                                               >  1:1257472/1‑140 (MQ=255)                                                                       gCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACg                                                               >  2:1283784/1‑140 (MQ=255)                                                                              aGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAg                                                        >  1:1155594/1‑140 (MQ=255)                                                                                cTCACCGGTGATAGTGTCCGCAGAAGGGCGCGAGACCCGAGCGCGTTCCCCGTTGCTTATTTTCACCAGACCTTTGGGGTTTAACGCCGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTTAAGAACGCCATCAGtt                                                      >  2:1217645/1‑140 (MQ=255)                                                                                cTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGtt                                                      >  2:1346408/1‑140 (MQ=255)                                                                                cTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGtt                                                      <  2:256746/140‑1 (MQ=255)                                                                                      gatgatAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGtt                                                <  1:562375/140‑1 (MQ=255)                                                                                      gatgatAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGtt                                                <  1:316142/140‑1 (MQ=255)                                                                                        tgatAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCa                                              <  2:930983/140‑1 (MQ=255)                                                                                         gatAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAg                                             >  2:1256901/1‑140 (MQ=255)                                                                                          atAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGa                                            <  2:695711/140‑1 (MQ=255)                                                                                          atAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGa                                            >  1:671844/1‑140 (MQ=255)                                                                                          atAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGa                                            <  1:717516/140‑1 (MQ=255)                                                                                          atAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGa                                            <  2:1537712/140‑1 (MQ=255)                                                                                           tAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGAc                                           <  1:538474/140‑1 (MQ=255)                                                                                           tAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGAc                                           <  2:1473363/140‑1 (MQ=255)                                                                                           tAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGAc                                           <  2:893546/140‑1 (MQ=255)                                                                                             gtgtCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACgg                                         <  1:16541/140‑1 (MQ=255)                                                                                              tgtCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGt                                        <  2:1585936/140‑1 (MQ=255)                                                                                               gtCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTa                                       >  2:224360/1‑140 (MQ=255)                                                                                               gtCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTa                                       <  2:2649/140‑1 (MQ=255)                                                                                               gtCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTa                                       >  2:776893/1‑140 (MQ=255)                                                                                                tCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTaa                                      >  2:603318/1‑140 (MQ=255)                                                                                                tCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTaa                                      <  2:51624/140‑1 (MQ=255)                                                                                                  cGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAAtt                                    >  1:1584376/1‑140 (MQ=255)                                                                                                  cGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAAtt                                    >  2:857955/1‑140 (MQ=255)                                                                                                  cGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAAtt                                    >  2:708672/1‑140 (MQ=255)                                                                                                  cGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAAtt                                    >  1:133328/1‑140 (MQ=255)                                                                                                   gCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTg                                   >  1:467407/1‑140 (MQ=255)                                                                                                    cAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGCCCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGt                                  <  2:1617166/140‑1 (MQ=255)                                                                                                     aGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGtt                                 <  1:1000685/140‑1 (MQ=255)                                                                                                     aGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGtt                                 >  2:1230508/1‑140 (MQ=255)                                                                                                        aGGACGCGAGACGCGAGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCAc                              >  1:390817/1‑140 (MQ=255)                                                                                                          gacgcgagacgcgaGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCt                            >  2:201941/1‑140 (MQ=255)                                                                                                          gacgcgagacgcgaGCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCt                            <  1:1521291/140‑1 (MQ=255)                                                                                                            cgcgagacgcgagCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTc                          >  1:813866/1‑140 (MQ=255)                                                                                                            cgcgagacgcgagCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTc                          >  2:1596299/1‑140 (MQ=255)                                                                                                               gagacgcgagCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGcc                       <  2:160265/140‑1 (MQ=255)                                                                                                                       agCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCac               >  2:1473039/1‑140 (MQ=255)                                                                                                                       agCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCac               <  1:1592219/140‑1 (MQ=255)                                                                                                                       agCGCGTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCac               >  2:113884/1‑140 (MQ=255)                                                                                                                          gcgTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCacgac            <  1:534994/140‑1 (MQ=255)                                                                                                                          gcgTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCacgac            <  2:1230119/140‑1 (MQ=255)                                                                                                                           cgTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCacgacg           >  2:1047518/1‑140 (MQ=255)                                                                                                                           cgTTCGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCacgacg           <  1:90108/140‑1 (MQ=255)                                                                                                                               cGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCACGAcggcgg       <  2:313020/140‑1 (MQ=255)                                                                                                                               cGCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGAACACCTTCGCCAAATTCACGAcggcgg       <  1:211423/140‑1 (MQ=255)                                                                                                                                gCCGTTGCTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCACGACGGCGGa      <  1:1346408/140‑1 (MQ=255)                                                                                                                                    ttgctTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCACGACGGCGGatcat  <  2:1155594/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACCAGACCTTTGCGTTTTAACGCTGCCAGCGCTTCACGCACCGAAGGACGCCCGACGTTAAAGAACGCCATCAGTTCGCGTTCAGACGGTAATTGTTCACCTTCGCCAAATTCACGACGGCGGATCAT  >  NC_000913/3374093‑3374363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑