Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,607,133	T→C	V228A (GTT→GCT)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,607,133	0	T	C	100.0%	309.4 / NA	85	V228A (GTT→GCT)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/41);  total (44/41)

GAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGG  >  NC_000913/3607000‑3607268                                                                                                                                      |                                                                                                                                        gAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAgcgc                                                                                                                                   >  1:1454298/1‑140 (MQ=255)  aaaCCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGcgcg                                                                                                                                  >  2:843408/1‑140 (MQ=255)    aCCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGtt                                                                                                                                >  1:816395/1‑140 (MQ=255)         aaTGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATgg                                                                                                                           >  2:97010/1‑140 (MQ=255)              gCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTg                                                                                                                      >  1:419731/1‑140 (MQ=255)               cGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGa                                                                                                                     <  1:708682/140‑1 (MQ=255)               cGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGa                                                                                                                     >  1:488240/1‑140 (MQ=255)                gTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGaa                                                                                                                    >  1:980456/1‑140 (MQ=255)                 tAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGaaa                                                                                                                   >  1:457639/1‑140 (MQ=255)                    ccGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCa                                                                                                                >  1:537222/1‑140 (MQ=255)                       cTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGaa                                                                                                             >  1:1503530/1‑140 (MQ=255)                             gTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGcc                                                                                                       <  2:844426/140‑1 (MQ=255)                              tGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGTCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCa                                                                                                      >  2:39167/1‑140 (MQ=255)                              tGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCACTGAAACCAGAAACCGCCa                                                                                                      >  2:555482/1‑140 (MQ=255)                               gCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCAc                                                                                                     <  1:639634/140‑1 (MQ=255)                                cACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCAcg                                                                                                    <  1:882272/140‑1 (MQ=255)                                  cTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCAcgcg                                                                                                  >  2:18527/1‑140 (MQ=255)                                  cTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCAcgcg                                                                                                  >  1:31362/1‑140 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGGGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGGGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:322563/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGTGGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:630583/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGTTGTTTTTGATTGGTGAAGGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  1:1442435/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:1355492/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  1:174142/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:415211/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:865662/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:381745/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:914635/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  2:1471570/140‑1 (MQ=255)                                   tGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  1:871494/140‑1 (MQ=255)                                   ggTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAGGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACgcgc                                                                                                 <  1:1611961/139‑1 (MQ=255)                                    gTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGcc                                                                                                >  2:1004846/1‑140 (MQ=255)                                    gTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGcc                                                                                                >  2:983069/1‑140 (MQ=255)                                    gTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGcc                                                                                                >  2:315806/1‑140 (MQ=255)                                    gTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGcc                                                                                                >  1:34651/1‑140 (MQ=255)                                    gTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGcc                                                                                                >  2:572336/1‑140 (MQ=255)                                     tttATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGTCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCt                                                                                               >  1:351061/1‑140 (MQ=255)                                       tATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGc                                                                                             <  1:425791/140‑1 (MQ=255)                                           ggCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTaa                                                                                         <  1:1106842/140‑1 (MQ=255)                                             cgcAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAgg                                                                                       >  2:267545/1‑140 (MQ=255)                                             cgcAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAgg                                                                                       >  1:69512/1‑140 (MQ=255)                                             cgcAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAgg                                                                                       >  2:679229/1‑140 (MQ=255)                                               cAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGt                                                                                     <  2:1454298/140‑1 (MQ=255)                                               cAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGt                                                                                     >  1:943526/1‑140 (MQ=255)                                               cAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGt                                                                                     >  1:1081152/1‑140 (MQ=255)                                                 aCGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGa                                                                                   <  1:1578436/140‑1 (MQ=255)                                                 aCGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGa                                                                                   <  2:965660/140‑1 (MQ=255)                                                            tGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAgg                                                                        <  2:537222/140‑1 (MQ=255)                                                            tGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAgg                                                                        <  1:774245/140‑1 (MQ=255)                                                              cGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTg                                                                      >  1:630347/1‑140 (MQ=255)                                                              cGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTg                                                                      >  2:1459787/1‑140 (MQ=255)                                                                aTGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGc                                                                    <  1:705961/140‑1 (MQ=255)                                                                      ttGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTaa                                                              <  1:379949/140‑1 (MQ=255)                                                                      ttGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTaa                                                              <  2:816395/140‑1 (MQ=255)                                                                            ctgTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCt                                                        >  1:1046644/1‑140 (MQ=255)                                                                             tgTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTg                                                       >  2:131227/1‑140 (MQ=255)                                                                             tgTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTg                                                       <  1:97010/140‑1 (MQ=255)                                                                             tgTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTg                                                       <  2:1503530/140‑1 (MQ=255)                                                                               tttttGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTgcg                                                     <  1:865699/140‑1 (MQ=255)                                                                                         gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAt                                           <  2:488240/140‑1 (MQ=255)                                                                                         gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAt                                           >  1:1141256/1‑140 (MQ=255)                                                                                         gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAt                                           >  2:1045169/1‑140 (MQ=255)                                                                                         gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAt                                           >  1:584927/1‑140 (MQ=255)                                                                                                 cTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGa                                   >  2:1356304/1‑140 (MQ=255)                                                                                                  tGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGaa                                  <  2:1637726/140‑1 (MQ=255)                                                                                                   ggAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAg                                 >  2:667874/1‑140 (MQ=255)                                                                                                   ggAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAg                                 >  1:781197/1‑140 (MQ=255)                                                                                                      aGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTcg                              <  2:369338/140‑1 (MQ=255)                                                                                                        gCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTcgcc                            <  2:890220/140‑1 (MQ=255)                                                                                                        gCTGGGCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTcgcc                            <  1:843408/140‑1 (MQ=255)                                                                                                           gggCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGCGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTcgccgcg                         >  2:1435183/1‑139 (MQ=255)                                                                                                            ggCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAg                        >  1:112775/1‑140 (MQ=255)                                                                                                            ggCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAg                        >  1:1092390/1‑140 (MQ=255)                                                                                                            ggCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAg                        >  1:649710/1‑140 (MQ=255)                                                                                                            ggCCGCCAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAg                        >  2:616282/1‑140 (MQ=255)                                                                                                             gccgccAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAgg                       <  2:1295977/140‑1 (MQ=255)                                                                                                              ccgccAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAATCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAggg                      >  2:1195581/1‑139 (MQ=255)                                                                                                               cgccAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTg                     >  1:1484143/1‑140 (MQ=255)                                                                                                                 ccggCCGCGCGCGTCGGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTggg                   <  2:1076185/137‑1 (MQ=255)                                                                                                                 ccAGCCGCGCGCGTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTggg                   >  1:1383378/1‑140 (MQ=255)                                                                                                                       gcgcgcgTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTtg             >  1:754453/1‑140 (MQ=255)                                                                                                                            cgTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTtgcctgc        <  2:772270/140‑1 (MQ=255)                                                                                                                            cgTCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTtgcctgc        <  1:667874/140‑1 (MQ=255)                                                                                                                              tCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCg      <  1:572336/140‑1 (MQ=255)                                                                                                                              tCAGGGCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCg      <  1:1004846/140‑1 (MQ=255)                                                                                                                                 gggCGCTAGCGCGTTAATGGCGCTGAATCCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACg   <  1:267545/140‑1 (MQ=255)                                                                                                                                 gggCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACg   <  1:659776/140‑1 (MQ=255)                                                                                                                                 gggCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACg   <  1:679229/140‑1 (MQ=255)                                                                                                                                  ggCGCTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACgg  >  2:1597443/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGG  >  NC_000913/3607000‑3607268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑