Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,200,155	(C)6→7	intergenic (+61/‑126)	yjaG → / → hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,200,149	1	.	C	100.0%	325.3 / NA	87	intergenic (+55/‑132)	yjaG/hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (41/46);  total (41/46)

ACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑CCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATG  >  NC_000913/4200010‑4200283                                                                                                                                              |                                                                                                                                        aCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCg                                                                                                                                          >  2:568213/1‑140 (MQ=255)  cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑c                                                                                                                                        >  1:1247305/1‑140 (MQ=255)     gATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccc                                                                                                                                     <  1:805088/140‑1 (MQ=255)       aatCGAGTTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGGGAGAGCAATATTGGGATAATTTTTCAGCAATAAGACCAGCAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccc                                                                                                                                   >  2:826063/2‑140 (MQ=255)          cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgt                                                                                                                                <  1:590901/140‑4 (MQ=255)          cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgt                                                                                                                                <  1:1160024/140‑4 (MQ=255)          cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgt                                                                                                                                <  2:454064/140‑4 (MQ=255)          cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgt                                                                                                                                <  2:194852/140‑4 (MQ=255)           gAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgtc                                                                                                                               <  1:755703/140‑5 (MQ=255)           gAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgtc                                                                                                                               <  1:597292/140‑5 (MQ=255)           gAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑cccccccgtc                                                                                                                               <  1:734010/140‑5 (MQ=255)             gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTg                                                                                                                              >  2:528973/1‑140 (MQ=255)             gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTg                                                                                                                              <  2:1459832/140‑1 (MQ=255)               tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGGTTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGt                                                                                                                            <  2:789776/140‑1 (MQ=255)               tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGt                                                                                                                            <  1:597262/140‑1 (MQ=255)               tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGt                                                                                                                            <  1:792135/140‑1 (MQ=255)                gATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTc                                                                                                                           <  1:121275/140‑1 (MQ=255)                 aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCt                                                                                                                          >  2:154450/1‑140 (MQ=255)                 aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCt                                                                                                                          >  2:537383/1‑140 (MQ=255)                           ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTgggg                                                                                                                >  1:995470/1‑140 (MQ=255)                           ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTgggg                                                                                                                >  2:67925/1‑140 (MQ=255)                             aGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTgggggg                                                                                                              >  1:1269143/1‑140 (MQ=255)                                   gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaa                                                                                                       >  1:618159/1‑140 (MQ=255)                                   gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaa                                                                                                       >  1:733941/1‑140 (MQ=255)                                   gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaa                                                                                                       >  1:29720/1‑140 (MQ=255)                                   gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaa                                                                                                       >  1:186697/1‑140 (MQ=255)                                    aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaa                                                                                                      <  1:1260457/140‑1 (MQ=255)                                    aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaa                                                                                                      >  1:562146/1‑140 (MQ=255)                                    aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaa                                                                                                      >  2:766329/1‑140 (MQ=255)                                      cTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaaaa                                                                                                    <  2:1188351/140‑1 (MQ=255)                                       tGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAg                                                                                                   >  1:518771/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  1:578890/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  2:1077758/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  1:653491/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  1:1215390/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  1:549993/1‑140 (MQ=255)                                         cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGGCTACATTTGGGGGGCG‑AAAAAAAGTg                                                                                                 >  1:30321/1‑140 (MQ=255)                                          gTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTgg                                                                                                <  1:1345367/140‑1 (MQ=255)                                          gTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTgg                                                                                                >  1:544455/1‑140 (MQ=255)                                           tGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGc                                                                                               >  2:332720/1‑140 (MQ=255)                                              ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                            >  2:1234183/1‑140 (MQ=255)                                              ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                            <  1:557116/140‑1 (MQ=255)                                              ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                            <  1:679284/140‑1 (MQ=255)                                                cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAAGTGGCTATc                                                                                           >  1:1005138/1‑140 (MQ=255)                                                 gggTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCgg                                                                                         <  1:67925/140‑1 (MQ=255)                                                    tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGc                                                                                      <  2:1103035/140‑1 (MQ=255)                                                    tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGc                                                                                      <  2:562146/140‑1 (MQ=255)                                                     gagaGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTT‑GGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  1:1269750/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  2:529950/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  2:416736/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  2:428507/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  1:15136/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  1:683033/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  1:1044149/140‑1 (MQ=255)                                                      agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                    <  1:260749/140‑1 (MQ=255)                                                        agCAAAATTTGTATAAATTTTCAATAATAAAACCAGAAAACGCGATTTAACGCCTGATTTGTCCTACCCGGAGACTTCCCTTTTAC‑CCCCCCCTCCGGGTTACCTTTTGGGGGCG‑GAAAAAAATGGCTATCGGTGCgtgg                                                                                  >  2:1194672/1‑139 (MQ=255)                                                           aaTATTTGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATg                                                                               <  1:826063/140‑1 (MQ=255)                                                                tGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGga                                                                          <  2:518771/140‑1 (MQ=255)                                                                  gTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGgaga                                                                        >  2:202604/1‑140 (MQ=255)                                                                      aaTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTg                                                                     <  2:1005138/140‑1 (MQ=255)                                                                                   tAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAttt                                                       >  1:538862/1‑140 (MQ=255)                                                                                          aGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGc                                                >  2:1434058/1‑140 (MQ=255)                                                                                           gAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCa                                               <  1:154450/140‑1 (MQ=255)                                                                                            aaaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                              <  2:1070449/140‑1 (MQ=255)                                                                                            aaaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                              >  2:228463/1‑140 (MQ=255)                                                                                             aaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                             <  2:77102/140‑1 (MQ=255)                                                                                             aaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                             <  2:995470/140‑1 (MQ=255)                                                                                             aaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                             <  2:618159/140‑1 (MQ=255)                                                                                                cGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGa                                          <  1:1194672/140‑1 (MQ=255)                                                                                                cGTGATTTAACACCTGATTTGTCGTACCTGTAGTCTTCTC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGTG‑AAAAATTGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTGAGTCGCACTCGa                                          <  2:1380196/140‑1 (MQ=255)                                                                                                 gTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGAt                                         >  1:1288662/1‑140 (MQ=255)                                                                                                        aaCGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGc                                  <  1:332720/140‑1 (MQ=255)                                                                                                        aaCGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGc                                  <  2:549993/140‑1 (MQ=255)                                                                                                         aCGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCa                                 >  2:114975/1‑140 (MQ=255)                                                                                                          cGCCTGATTTGTCGTCCCTGGAGTCTTCCC‑TTCCGCCCCCCCGTCTGGCTTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                                <  2:1269143/140‑1 (MQ=255)                                                                                                          cGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                                <  1:1434058/140‑1 (MQ=255)                                                                                                          cGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                                <  2:702486/140‑1 (MQ=255)                                                                                                          cGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                                <  1:568213/140‑1 (MQ=255)                                                                                                               gATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                           >  1:974071/1‑140 (MQ=255)                                                                                                               gATTTGTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                           <  1:751207/140‑1 (MQ=255)                                                                                                                    gTCGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                      <  2:1215390/140‑1 (MQ=255)                                                                                                                      cGTACCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAAcaca                    <  2:1222725/140‑1 (MQ=255)                                                                                                                         gccTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTg                 <  2:30321/139‑1 (MQ=255)                                                                                                                         aCCTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTg                 <  2:198360/140‑1 (MQ=255)                                                                                                                          ccTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGt                >  2:1123520/1‑140 (MQ=255)                                                                                                                          ccTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGt                >  1:1204613/1‑140 (MQ=255)                                                                                                                           cTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa               <  2:1408077/140‑1 (MQ=255)                                                                                                                           cTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa               <  1:528973/140‑1 (MQ=255)                                                                                                                           cTGGAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa               <  1:1448889/140‑1 (MQ=255)                                                                                                                             ggAGTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAg             >  2:902479/1‑140 (MQ=255)                                                                                                                                gTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt          <  2:1247305/140‑1 (MQ=255)                                                                                                                                gTCTTCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt          <  1:537383/140‑1 (MQ=255)                                                                                                                                   ttCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc       >  1:1018689/1‑140 (MQ=255)                                                                                                                                   ttCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc       >  1:1071801/1‑140 (MQ=255)                                                                                                                                   ttCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc       >  2:64027/1‑140 (MQ=255)                                                                                                                                   ttCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGGATGCAGGAGTGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc       >  2:89526/1‑140 (MQ=255)                                                                                                                                    tCCC‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACt      >  1:1391465/1‑140 (MQ=255)                                                                                                                                     ccc‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt     >  2:835567/1‑140 (MQ=255)                                                                                                                                     ccc‑TTTCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt     >  2:577854/1‑140 (MQ=255)                                                                                                                                         tttCGCCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATg  >  2:166214/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                        ACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC‑TTTCG‑CCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATG  >  NC_000913/4200010‑4200283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑