Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	43,291	T→C	V35A (GTC→GCC)	fixB →	putative electron transfer flavoprotein FixB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	43,291	0	T	C	100.0%	234.2 / NA	62	V35A (GTC→GCC)	fixB	putative electron transfer flavoprotein FixB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

CGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGTCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACGGCG  >  NC_000913/43159‑43425                                                                                                                                     |                                                                                                                                       cGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAat                                                                                                                                 >  2:909009/1‑140 (MQ=255)    aaaGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAatgat                                                                                                                              <  2:285758/140‑1 (MQ=255)      aGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGc                                                                                                                            <  1:560923/140‑1 (MQ=255)           tttAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCGGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACg                                                                                                                       <  1:1030268/140‑1 (MQ=255)           tttAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACg                                                                                                                       >  2:598097/1‑140 (MQ=255)               aTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGgcgc                                                                                                                   <  2:1390183/140‑1 (MQ=255)               aTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGgcgc                                                                                                                   <  1:995713/140‑1 (MQ=255)               aTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGgcgc                                                                                                                   <  1:166921/140‑1 (MQ=255)               aTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGgcgc                                                                                                                   <  1:1261862/140‑1 (MQ=255)                  aCAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGTCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGcaca                                                                                                                <  1:945877/140‑1 (MQ=255)                    aGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAgg                                                                                                              >  2:941703/1‑140 (MQ=255)                    aGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAgg                                                                                                              <  1:1051942/140‑1 (MQ=255)                        gATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAAt                                                                                                          <  1:1066680/140‑1 (MQ=255)                             tATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGc                                                                                                     >  2:257366/1‑140 (MQ=255)                               tGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTc                                                                                                   <  2:139763/140‑1 (MQ=255)                                     cGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCt                                                                                             >  1:701500/1‑140 (MQ=255)                                      gTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTa                                                                                            >  2:886498/1‑140 (MQ=255)                                       ttttCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTaa                                                                                           >  1:1175069/1‑140 (MQ=255)                                        tttCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAAt                                                                                          <  2:455104/140‑1 (MQ=255)                                             cAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGt                                                                                     <  2:2255/140‑1 (MQ=255)                                                 tCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGACAATCCAGCTCGGCGCTAATCATGTCTgg                                                                                 >  2:1078656/1‑140 (MQ=255)                                                    gggTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGaaa                                                                              >  1:1162565/1‑140 (MQ=255)                                                     ggTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAAt                                                                             >  2:1427310/1‑140 (MQ=255)                                                     ggTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAAt                                                                             >  1:944989/1‑140 (MQ=255)                                                            aGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACgg                                                                      <  1:886498/140‑1 (MQ=255)                                                               gATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCaa                                                                   >  1:1360624/1‑140 (MQ=255)                                                                aTACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCaaa                                                                  <  1:193505/140‑1 (MQ=255)                                                                 tACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAAc                                                                 >  2:651167/1‑140 (MQ=255)                                                                 tACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAAc                                                                 >  1:859410/1‑140 (MQ=255)                                                                 tACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAAc                                                                 >  1:1292726/1‑140 (MQ=255)                                                                   ccccTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCg                                                               >  2:796672/1‑140 (MQ=255)                                                                   ccccTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCg                                                               >  2:78436/1‑140 (MQ=255)                                                                         ctcGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGAt                                                         <  1:105119/140‑1 (MQ=255)                                                                         ctcGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGAt                                                         <  2:1175069/140‑1 (MQ=255)                                                                             tCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGga                                                     <  2:503286/140‑1 (MQ=255)                                                                             tCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGga                                                     >  2:1232607/1‑140 (MQ=255)                                                                                gCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGgatga                                                  >  2:670347/1‑140 (MQ=255)                                                                                  cGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCTGACGATCGGATGATc                                                <  2:1325073/140‑1 (MQ=255)                                                                                       cTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGACAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGa                                           <  1:1078656/140‑1 (MQ=255)                                                                                           tgaACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTAc                                       >  1:288369/1‑140 (MQ=255)                                                                                           tgaACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTAc                                       >  2:978693/1‑140 (MQ=255)                                                                                              aCGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGcc                                    <  1:670347/140‑1 (MQ=255)                                                                                               cGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCg                                   <  1:1070390/140‑1 (MQ=255)                                                                                                ggTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCgg                                  >  2:75747/1‑140 (MQ=255)                                                                                                  tGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGgtg                                >  1:296929/1‑140 (MQ=255)                                                                                                    cgcAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTc                              >  1:1024711/1‑140 (MQ=255)                                                                                                    cgcAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTc                              >  1:687834/1‑140 (MQ=255)                                                                                                    cgcAGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTc                              <  1:257366/140‑1 (MQ=255)                                                                                                       aGGCTTTAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATg                           <  1:687729/140‑1 (MQ=255)                                                                                                            ttAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGa                      <  1:909009/140‑1 (MQ=255)                                                                                                            ttAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGa                      >  2:878951/1‑140 (MQ=255)                                                                                                            ttAGCTAATCAAATCAACACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGa                      >  1:260991/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  1:126763/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  2:536582/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  2:520006/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  1:800532/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  1:1135154/1‑140 (MQ=255)                                                                                                                   atcaaatcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTAtt               >  1:202296/1‑140 (MQ=255)                                                                                                                         tcaaCACCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAg         >  2:1077479/1‑140 (MQ=255)                                                                                                                             cacCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGAAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACg     >  2:989572/1‑140 (MQ=255)                                                                                                                              acCTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACgg    >  2:563213/1‑140 (MQ=255)                                                                                                                                cTTTGCCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACGgcg  >  2:775557/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAACACCTTTGTCCTCAATGATGCCGACGGCGCACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGAAATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACGGCG  >  NC_000913/43159‑43425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑