BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,225	A→G	L132P (CTG→CCG)	acrA ←	multidrug efflux pump membrane fusion lipoprotein AcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,225	0	A	G	100.0%	421.9 / NA	110	L132P (CTG→CCG)	acrA	multidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/54); total (56/54)

Only 100 of 110 total aligned reads displayed.

GGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGAT  >  NC_000913/485089‑485361

ggTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGtt                                                                                                                                       >  2:50360/1‑140 (MQ=255)

ggTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGtt                                                                                                                                       >  1:1213679/1‑140 (MQ=255)

ggTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGtt                                                                                                                                       >  1:843993/1‑140 (MQ=255)

    aCTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTg                                                                                                                                   >  2:983640/1‑140 (MQ=255)

    aCTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTg                                                                                                                                   >  1:1376395/1‑140 (MQ=255)

       ttGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATa                                                                                                                                >  1:65627/1‑140 (MQ=255)

         ggTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAAc                                                                                                                              >  2:460598/1‑140 (MQ=255)

            gtAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGAt                                                                                                                           <  2:1487339/140‑1 (MQ=255)

            gtAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGAt                                                                                                                           <  2:296526/140‑1 (MQ=255)

            gtAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGAt                                                                                                                           <  1:1460321/140‑1 (MQ=255)

             tAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGAtt                                                                                                                          <  1:117526/140‑1 (MQ=255)

             tAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGAtt                                                                                                                          <  2:262354/140‑1 (MQ=255)

                     aTTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCa                                                                                                                  >  2:15861/1‑140 (MQ=255)

                     aTTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCa                                                                                                                  <  2:22154/140‑1 (MQ=255)

                     aTTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCa                                                                                                                  >  2:941747/1‑140 (MQ=255)

                         aTCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTg                                                                                                              >  1:1060098/1‑140 (MQ=255)

                          tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTgc                                                                                                             >  1:660900/1‑140 (MQ=255)

                          tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTgc                                                                                                             >  2:192800/1‑140 (MQ=255)

                          tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTgc                                                                                                             >  1:561534/1‑140 (MQ=255)

                          tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTgc                                                                                                             <  1:1131657/140‑1 (MQ=255)

                           ccGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTgcg                                                                                                            <  2:1349385/140‑1 (MQ=255)

                              cgcAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGa                                                                                                         >  2:1380985/1‑140 (MQ=255)

                              cgcAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGa                                                                                                         >  1:438234/1‑140 (MQ=255)

                                 aGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGatat                                                                                                      <  1:424769/140‑1 (MQ=255)

                                             aGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCt                                                                                          >  2:55257/1‑140 (MQ=255)

                                               cTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTgg                                                                                        >  2:34198/1‑140 (MQ=255)

                                               cTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTgg                                                                                        >  1:1215746/1‑140 (MQ=255)

                                                  tCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCt                                                                                     <  2:948124/140‑1 (MQ=255)

                                                     ccgcAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTc                                                                                  >  2:753050/1‑140 (MQ=255)

                                                        cAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCCGGGCTTTCGcc                                                                               >  2:830844/1‑140 (MQ=255)

                                                           ttACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGa                                                                            <  1:373381/140‑1 (MQ=255)

                                                             aCCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATc                                                                          <  2:1213679/140‑1 (MQ=255)

                                                             aCCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATc                                                                          >  2:1428355/1‑140 (MQ=255)

                                                              ccGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCa                                                                         >  2:40478/1‑140 (MQ=255)

                                                                   gcaTTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCttt                                                                    >  1:182700/1‑140 (MQ=255)

                                                                     aTTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCg                                                                  <  1:1262616/140‑1 (MQ=255)

                                                                       tCGCCTGTTGTGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  1:354081/140‑1 (MQ=255)

                                                                       tCGCCTGTTGTGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  1:1318414/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCTCCTTTCGCa                                                                <  2:611503/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  2:1425446/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  2:1406693/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  1:984169/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  1:50360/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  2:65627/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  2:691531/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                <  2:786163/140‑1 (MQ=255)

                                                                       tCGCCTGTTGCGCATCAG‑CAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCAc                                                               >  1:783549/1‑140 (MQ=255)

                                                                         gCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGCTAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACt                                                              >  1:1172919/1‑140 (MQ=255)

                                                                           cTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGt                                                            <  2:438234/140‑1 (MQ=255)

                                                                           cTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGt                                                            <  1:55257/140‑1 (MQ=255)

                                                                              ttGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGt                                                         >  2:1203210/1‑140 (MQ=255)

                                                                               tGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa                                                        <  1:941747/140‑1 (MQ=255)

                                                                                     tcaaccAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGc                                                  >  1:611167/5‑140 (MQ=255)

                                                                                        gCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg                                               >  1:485627/1‑140 (MQ=255)

                                                                                         ccAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa                                              <  2:349491/140‑1 (MQ=255)

                                                                                         ccAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa                                              >  1:1126620/1‑140 (MQ=255)

                                                                                          cAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                             <  1:218509/140‑1 (MQ=255)

                                                                                          cAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                             <  2:549510/140‑1 (MQ=255)

                                                                                          cAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                             <  2:843993/140‑1 (MQ=255)

                                                                                                cTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGTGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg                                       <  2:960047/140‑1 (MQ=255)

                                                                                                cTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg                                       <  1:192800/140‑1 (MQ=255)

                                                                                                cTGATCGTACTCTTGCTTACAGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg                                       <  2:1060098/140‑1 (MQ=255)

                                                                                                  gATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCa                                     <  2:1376395/140‑1 (MQ=255)

                                                                                                   aTCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg                                    >  2:103189/1‑140 (MQ=255)

                                                                                                   aTCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg                                    >  2:334693/1‑140 (MQ=255)

                                                                                                   aTCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg                                    >  2:1352648/1‑140 (MQ=255)

                                                                                                    tCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg                                   <  1:1428355/140‑1 (MQ=255)

                                                                                                      gTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGAt                                 <  2:783549/140‑1 (MQ=255)

                                                                                                      gTACTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGAt                                 <  2:508385/140‑1 (MQ=255)

                                                                                                        aCTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATTACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa                               <  2:1327462/140‑1 (MQ=255)

                                                                                                        aCTCTTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa                               <  1:34198/140‑1 (MQ=255)

                                                                                                          tctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAAt                             <  2:978552/140‑1 (MQ=255)

                                                                                                          tctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAAt                             <  2:726104/140‑1 (MQ=255)

                                                                                                          tctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAAt                             <  2:57682/140‑1 (MQ=255)

                                                                                                           ctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATc                            >  2:428653/1‑140 (MQ=255)

                                                                                                           ctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATc                            <  1:15861/140‑1 (MQ=255)

                                                                                                           ctTGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATc                            <  2:182700/140‑1 (MQ=255)

                                                                                                             tGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTg                          >  1:885380/1‑140 (MQ=255)

                                                                                                             tGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTg                          >  1:1332139/1‑140 (MQ=255)

                                                                                                             tGCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTg                          >  2:645046/1‑140 (MQ=255)

                                                                                                              gCTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGa                         <  2:805707/140‑1 (MQ=255)

                                                                                                               cTTACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAt                        <  1:1220124/140‑1 (MQ=255)

                                                                                                                 tACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                      <  2:558304/140‑1 (MQ=255)

                                                                                                                 tACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                      >  2:1392713/1‑140 (MQ=255)

                                                                                                                 tACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                      <  2:1068083/140‑1 (MQ=255)

                                                                                                                 tACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                      >  1:758996/1‑140 (MQ=255)

                                                                                                                 tACTGATGTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGCCGCAGGATCAATCTGATag                      >  2:823064/1‑140 (MQ=255)

                                                                                                                        gTACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga               >  2:6898/1‑140 (MQ=255)

                                                                                                                         tACTGAGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGACTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGac              >  1:1290138/1‑140 (MQ=255)

                                                                                                                              aGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTg         >  1:363396/1‑140 (MQ=255)

                                                                                                                              aGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTg         >  1:642773/1‑140 (MQ=255)

                                                                                                                              aGTACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTg         >  2:1208790/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt       >  2:1405015/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt       >  2:718796/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt       >  2:869408/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt       >  2:1189228/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCt       >  1:280905/1‑140 (MQ=255)

                                                                                                                                tACCGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATACTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACAGTCGTATGTCGCCTGATAGGGCGCAGGCACAATCTGTTACAGACAGACACCTGCt       >  2:1127653/1‑140 (MQ=255)

                                                                                                                                   cGAGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCg    <  2:125479/140‑1 (MQ=255)

                                                                                                                                     aGCGGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGAt  >  1:685579/1‑140 (MQ=255)

GGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGAT  >  NC_000913/485089‑485361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`