Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,304,978	A→G	G540G (GGT→GGC)	yjcS ←	alkyl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,304,978	0	A	G	100.0%	296.6 / NA	78	G540G (GGT→GGC)	yjcS	alkyl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/45);  total (33/45)

TCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG  >  NC_000913/4304842‑4305113                                                                                                                                         |                                                                                                                                        tCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCg                                                                                                                                      >  2:70284/1‑140 (MQ=255) tCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGAGAGCCg                                                                                                                                      >  2:1087385/1‑140 (MQ=255)    ggTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCggtg                                                                                                                                   >  1:866821/1‑140 (MQ=255)        gAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGc                                                                                                                               <  1:418245/140‑1 (MQ=255)         aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGTCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGcc                                                                                                                              <  1:165825/140‑1 (MQ=255)         aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGcc                                                                                                                              >  2:279173/1‑140 (MQ=255)         aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGcc                                                                                                                              <  1:549519/140‑1 (MQ=255)         aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGAGGTGcc                                                                                                                              >  2:578984/1‑140 (MQ=255)          ggTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCg                                                                                                                             <  1:191458/140‑1 (MQ=255)           gTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCAGTGGTGCCGt                                                                                                                            <  2:458033/140‑1 (MQ=255)             tATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTgg                                                                                                                          <  1:857691/140‑1 (MQ=255)               tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCt                                                                                                                        >  2:983715/1‑140 (MQ=255)               tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCt                                                                                                                        >  2:887341/1‑140 (MQ=255)                cGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTACGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTg                                                                                                                       <  1:894092/140‑1 (MQ=255)                   cGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAAc                                                                                                                    <  1:218683/140‑1 (MQ=255)                     ttGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCGTAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACtt                                                                                                                  <  2:717804/140‑1 (MQ=255)                     ttGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACtt                                                                                                                  >  1:1187163/1‑140 (MQ=255)                      tGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTa                                                                                                                 >  1:506978/1‑140 (MQ=255)                          cATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGATCAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCa                                                                                                             <  2:1185754/140‑1 (MQ=255)                          cATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCa                                                                                                             <  1:964676/140‑1 (MQ=255)                          cATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCa                                                                                                             <  2:760514/140‑1 (MQ=255)                          cATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCa                                                                                                             <  1:585772/140‑1 (MQ=255)                          cATATTGAAGTAAAGCCTGATATTGTTTCCCGCAGCTTTCGCGCTATAGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGTACTTATGct                                                                                                             <  2:320944/140‑2 (MQ=255)                                  aGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTcg                                                                                                     >  1:1151264/1‑140 (MQ=255)                                   gTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTcgc                                                                                                    <  1:1125067/140‑1 (MQ=255)                                    ttCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTcgcg                                                                                                   <  2:18513/140‑1 (MQ=255)                                      cAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCa                                                                                                 >  1:196094/1‑140 (MQ=255)                                       aGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAg                                                                                                >  1:477806/1‑140 (MQ=255)                                       aGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAg                                                                                                <  2:1319468/140‑1 (MQ=255)                                       aGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAg                                                                                                >  1:656963/1‑140 (MQ=255)                                             atatTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCttt                                                                                          <  1:349255/140‑1 (MQ=255)                                                 ttttTCCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTcgcg                                                                                      <  1:527430/140‑1 (MQ=255)                                                 ttttACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTcgcg                                                                                      <  2:467926/140‑1 (MQ=255)                                                  tttACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCgcgc                                                                                     <  1:647490/140‑1 (MQ=255)                                                  tttACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCgcgc                                                                                     <  2:866821/140‑1 (MQ=255)                                                      cccGCAGCTTTCGCGCTATCGAGGCGAACGGTCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGt                                                                                 <  2:897698/140‑1 (MQ=255)                                                      cccGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGt                                                                                 <  2:969220/140‑1 (MQ=255)                                                      cccGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGt                                                                                 <  2:929076/140‑1 (MQ=255)                                                      cccGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGt                                                                                 <  2:1277283/140‑1 (MQ=255)                                                       ccGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTc                                                                                >  2:778604/1‑140 (MQ=255)                                                         gCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAg                                                                              >  2:650592/1‑140 (MQ=255)                                                         gCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAg                                                                              >  1:382053/1‑140 (MQ=255)                                                                 cgcgCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAAc                                                                      <  1:1116529/140‑1 (MQ=255)                                                                  gcgcTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAAcc                                                                     <  1:1493911/140‑1 (MQ=255)                                                                   cgcTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACcg                                                                    <  1:830273/140‑1 (MQ=255)                                                                     cTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACcgcg                                                                  <  1:70284/140‑1 (MQ=255)                                                                             gCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTgg                                                          <  2:1151264/140‑1 (MQ=255)                                                                             gCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTgg                                                          <  2:91308/140‑1 (MQ=255)                                                                             gCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTgg                                                          >  1:143184/1‑140 (MQ=255)                                                                                 aCGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGa                                                      <  2:813043/140‑1 (MQ=255)                                                                                  cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  2:1106318/1‑140 (MQ=255)                                                                                  cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  1:621226/1‑140 (MQ=255)                                                                                  cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  1:1483129/1‑140 (MQ=255)                                                                                  cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  1:789877/1‑140 (MQ=255)                                                                                   ggCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACt                                                    <  2:506978/140‑1 (MQ=255)                                                                                   ggCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACt                                                    <  1:678563/140‑1 (MQ=255)                                                                                   gcccATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACt                                                    <  2:1390600/138‑1 (MQ=255)                                                                                     ccATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCg                                                  <  1:694844/140‑1 (MQ=255)                                                                                             tCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATa                                          >  2:174353/1‑140 (MQ=255)                                                                                              cGAGCAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAg                                         <  2:825818/140‑1 (MQ=255)                                                                                               gAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCTGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAgt                                        >  1:405681/1‑139 (MQ=255)                                                                                               gAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGc                                        >  2:263666/1‑140 (MQ=255)                                                                                               gAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGc                                        >  2:1238412/1‑140 (MQ=255)                                                                                               gAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGc                                        >  2:1207657/1‑140 (MQ=255)                                                                                                aaCAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGcc                                       >  2:827276/1‑140 (MQ=255)                                                                                                   aGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAg                                    <  2:1221424/140‑1 (MQ=255)                                                                                                     cATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCt                                  >  1:345678/1‑140 (MQ=255)                                                                                                        ttCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGtt                               <  1:578984/140‑1 (MQ=255)                                                                                                               gacATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGtt                        >  1:1089911/1‑140 (MQ=255)                                                                                                                  aTCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTAtt                     <  1:778604/140‑1 (MQ=255)                                                                                                                    cccGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCg                   >  1:1256074/1‑140 (MQ=255)                                                                                                                           aTGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAg            >  2:1171349/1‑140 (MQ=255)                                                                                                                           aTGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAg            >  1:258086/1‑140 (MQ=255)                                                                                                                            tGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa           <  2:477806/140‑1 (MQ=255)                                                                                                                            tGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa           >  1:781917/1‑140 (MQ=255)                                                                                                                             ggTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAt          <  2:390328/140‑1 (MQ=255)                                                                                                                              gtgtCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAtt         >  2:15706/1‑140 (MQ=255)                                                                                                                                 tCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt      >  1:718949/1‑140 (MQ=255)                                                                                                                                 tCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttctt      <  1:1087385/140‑1 (MQ=255)                                                                                                                                   cGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcg    <  2:382053/140‑1 (MQ=255)                                                                                                                                     gggAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg  <  1:922468/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG  >  NC_000913/4304842‑4305113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑