Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,551,796	A→G	D54G (GAC→GGC)	uxuA →	D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,551,796	0	A	G	100.0%	325.1 / NA	86	D54G (GAC→GGC)	uxuA	D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/56);  total (30/56)

GCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCA  >  NC_000913/4551661‑4551930                                                                                                                                        |                                                                                                                                       gCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCa                                                                                                                                    >  1:104180/1‑140 (MQ=255)  cccAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAg                                                                                                                                   <  1:453544/140‑1 (MQ=255)    cAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGc                                                                                                                                 >  1:1310387/1‑140 (MQ=255)     aaaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCGTTGAAGGCGCAGGcc                                                                                                                                <  1:694495/140‑1 (MQ=255)      aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCt                                                                                                                               >  1:975202/1‑140 (MQ=255)      aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCt                                                                                                                               >  1:161460/1‑140 (MQ=255)         gATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGgt                                                                                                                            <  1:1395915/140‑1 (MQ=255)          aTCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGgtg                                                                                                                           <  1:985261/140‑1 (MQ=255)           tCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGgtgt                                                                                                                          >  2:1035588/1‑140 (MQ=255)            ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGtgtg                                                                                                                         >  1:423582/1‑140 (MQ=255)            ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGtgtg                                                                                                                         >  2:1301474/1‑140 (MQ=255)            ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGtgtg                                                                                                                         <  2:1454843/140‑1 (MQ=255)            ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGtgtg                                                                                                                         >  2:187569/1‑140 (MQ=255)            ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAAGCCTGGtgtg                                                                                                                         >  2:1419525/1‑140 (MQ=255)              ggTTTCTTTAGCTGATGTGCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:1167271/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTGTGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:217690/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:586528/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:993171/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:804700/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:726754/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:747187/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:775016/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:789622/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:847017/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:88943/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:984497/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:1108400/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:116592/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  2:1448479/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:1312362/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:1329961/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:1296197/140‑1 (MQ=255)              ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGt                                                                                                                       <  1:1094419/140‑1 (MQ=255)                              gTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGt                                                                                                       <  2:575183/140‑1 (MQ=255)                               tCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTg                                                                                                      <  2:1176798/140‑1 (MQ=255)                                 cGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGGGTGGTCTGTCGTAGAAAGCGTGcc                                                                                                    >  1:1323884/1‑140 (MQ=255)                                         gggcgCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACg                                                                                            <  1:584236/140‑1 (MQ=255)                                                aCTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGatat                                                                                     <  1:435936/140‑1 (MQ=255)                                                 cTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATc                                                                                    >  2:373544/1‑140 (MQ=255)                                                 cTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATc                                                                                    <  2:830381/140‑1 (MQ=255)                                                  tGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCa                                                                                   <  1:1330632/140‑1 (MQ=255)                                                      gTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAAc                                                                               <  1:87703/140‑1 (MQ=255)                                                         gTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCca                                                                            >  2:591289/1‑140 (MQ=255)                                                          ttACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCcac                                                                           <  2:812088/140‑1 (MQ=255)                                                          ttACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCcac                                                                           <  2:1311739/140‑1 (MQ=255)                                                             ccGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATGGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACt                                                                        <  2:382909/140‑1 (MQ=255)                                                              cgcgCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTg                                                                       >  2:1133057/1‑140 (MQ=255)                                                               gcgcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTgg                                                                      <  2:288010/140‑1 (MQ=255)                                                               gcgcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTgg                                                                      <  2:975202/140‑1 (MQ=255)                                                                 gcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCa                                                                    <  2:1310387/140‑1 (MQ=255)                                                                 gcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCa                                                                    <  1:394406/140‑1 (MQ=255)                                                                  cTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCACAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCaa                                                                   >  1:396106/1‑140 (MQ=255)                                                                     cACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTa                                                                <  1:1009742/140‑1 (MQ=255)                                                                      aCCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTAt                                                               <  1:1094311/140‑1 (MQ=255)                                                                      aCCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTAt                                                               <  2:423582/140‑1 (MQ=255)                                                                          tatCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGTTCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGc                                                           <  2:1323884/140‑1 (MQ=255)                                                                           atCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCa                                                          >  1:791739/1‑140 (MQ=255)                                                                             cccGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGt                                                        >  2:1260527/1‑140 (MQ=255)                                                                               cGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTgg                                                      >  2:1361401/1‑140 (MQ=255)                                                                               cGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTgg                                                      >  2:361886/1‑140 (MQ=255)                                                                                 aaCGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGAt                                                    >  1:1323283/1‑140 (MQ=255)                                                                                    ggCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGc                                                 <  1:964992/140‑1 (MQ=255)                                                                                     gCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAATATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCt                                                <  1:390728/140‑1 (MQ=255)                                                                                                tCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACACACACTGGCAACTATGAGCAGTGGATCGCTAACTATcagca                                     >  2:919381/1‑140 (MQ=255)                                                                                                   gTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAc                                  <  1:1131432/140‑1 (MQ=255)                                                                                                    ttGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAct                                 >  2:1211403/1‑140 (MQ=255)                                                                                                    ttGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAct                                 >  1:312860/1‑140 (MQ=255)                                                                                                    ttGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAct                                 <  2:1404242/140‑1 (MQ=255)                                                                                                     tGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGActc                                >  1:804979/1‑140 (MQ=255)                                                                                                     tGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGActc                                >  1:771746/1‑140 (MQ=255)                                                                                                         gagaTCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTgcg                            <  1:1182525/140‑1 (MQ=255)                                                                                                            aTCCTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCaa                         >  2:106164/1‑140 (MQ=255)                                                                                                              ccTCAAACGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAAcc                       >  1:267374/1‑140 (MQ=255)                                                                                                                   aaCGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgcg                  <  1:1260527/140‑1 (MQ=255)                                                                                                                   aaCGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgcg                  <  1:33982/140‑1 (MQ=255)                                                                                                                     cGCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCa                <  2:1323283/140‑1 (MQ=255)                                                                                                                      gCAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAg               >  1:204649/1‑140 (MQ=255)                                                                                                                       cAAGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCCCACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAgt              <  2:455233/140‑1 (MQ=255)                                                                                                                         aGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAgtgt            <  1:1269308/140‑1 (MQ=255)                                                                                                                         aGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAgtgt            <  1:1281773/140‑1 (MQ=255)                                                                                                                         aGGCGATCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAgtgt            <  2:791739/140‑1 (MQ=255)                                                                                                                              aTCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCAt       >  2:330721/1‑140 (MQ=255)                                                                                                                               tCATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCAtt      >  1:44942/1‑140 (MQ=255)                                                                                                                                cATTGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTc     >  1:234005/1‑140 (MQ=255)                                                                                                                                  ttGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGc   <  2:1256366/140‑1 (MQ=255)                                                                                                                                   tGAAGGCGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCa  <  1:1361401/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCA  >  NC_000913/4551661‑4551930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑