Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,055	A→G	V110A (GTA→GCA)	ybdK ←	carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,055	0	A	G	100.0%	164.9 / NA	46	V110A (GTA→GCA)	ybdK	carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/15);  total (31/15)

GCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAA  >  NC_000913/606925‑607190                                                                                                                                   |                                                                                                                                        gCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGAc                                                                                                                                >  2:762149/1‑140 (MQ=255)      gcagcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGc                                                                                                                           <  1:727187/140‑1 (MQ=255)       cagcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGcc                                                                                                                          >  2:1081010/1‑140 (MQ=255)        agcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCACATGCCTCCTGACGCTGCCa                                                                                                                         <  1:478453/140‑1 (MQ=255)          caAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCAtt                                                                                                                       <  1:3528/140‑1 (MQ=255)            aataaatGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTc                                                                                                                     >  2:458869/1‑140 (MQ=255)                aatGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGaa                                                                                                                 <  1:1312630/140‑1 (MQ=255)                 atGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGaaa                                                                                                                <  2:153551/140‑1 (MQ=255)                    gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             <  2:721817/140‑1 (MQ=255)                    gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             >  1:403655/1‑140 (MQ=255)                    gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             <  1:559346/140‑1 (MQ=255)                       tCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGgtg                                                                                                          <  1:1433896/140‑1 (MQ=255)                         aTCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGgtgcg                                                                                                        >  2:1295827/1‑140 (MQ=255)                               aCTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCAc                                                                                                  >  2:1302962/1‑140 (MQ=255)                                    cgcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCAccgccg                                                                                             >  2:524372/1‑140 (MQ=255)                                     gcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCTTTTCTGAAACGGGTGCGTGCCACcgccgc                                                                                            <  2:788564/140‑1 (MQ=255)                                     gcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACcgccgc                                                                                            >  2:737055/1‑140 (MQ=255)                                      cAGCCAACATGGACATGCTGACCAAAACCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCa                                                                                           <  1:190046/140‑1 (MQ=255)                                             cATGGTCATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTcc                                                                                    >  2:1089574/1‑140 (MQ=255)                                                 gACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgat                                                                                >  2:677576/1‑140 (MQ=255)                                                   cATGCTGCCAAAAACCGGTCGCCTGCTGAAGGACATACCCAAGGTTGTCCGGCGTGCGTTGATAGCGTTCGTTTTCGCATGCCCCCCGGCCCTGCAATTTTTGAAACGGGTGCGTCCCACCGCCGCAAATTTCCAgatga                                                                              <  2:12915/140‑1 (MQ=255)                                                   cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAgatga                                                                              >  1:1063013/1‑140 (MQ=255)                                                   cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              <  1:594630/140‑1 (MQ=255)                                                   cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              >  2:1359013/1‑140 (MQ=255)                                                   cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              >  2:833228/1‑140 (MQ=255)                                                     tGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGatgatg                                                                            >  2:1465736/1‑140 (MQ=255)                                                      gCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATgg                                                                           >  2:1467353/1‑140 (MQ=255)                                                       cTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAATTTTCCAGATGATGGt                                                                          >  2:260109/1‑140 (MQ=255)                                                       cTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGt                                                                          >  1:694174/1‑140 (MQ=255)                                                         gACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCt                                                                        >  1:1407527/1‑140 (MQ=255)                                                              aaaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATGGCGTTCGTTACCGCATGCCTCCTGACGCTGCCTTTTCTGAAAGGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGc                                                                   <  2:350002/140‑1 (MQ=255)                                                              aaaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGc                                                                   >  2:325408/1‑140 (MQ=255)                                                               aaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGct                                                                  >  2:554440/1‑140 (MQ=255)                                                                    gTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGctgcctg                                                             >  2:286363/1‑140 (MQ=255)                                                                    gTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGctgcctg                                                             <  1:1081010/140‑1 (MQ=255)                                                                          tgctgAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATAc                                                       >  2:1197595/1‑140 (MQ=255)                                                                           gctgAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACg                                                      <  2:133020/140‑1 (MQ=255)                                                                                   agaTAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTg                                              <  1:505439/140‑1 (MQ=255)                                                                                         ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGc                                        >  2:95224/1‑140 (MQ=255)                                                                                         ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGc                                        >  2:570420/1‑140 (MQ=255)                                                                                              gTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGaaa                                   >  2:1177966/1‑140 (MQ=255)                                                                                                         tgcgtTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                        >  1:316854/1‑140 (MQ=255)                                                                                                         tgcgtTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                        >  2:904205/1‑140 (MQ=255)                                                                                                           cgtTGATAGCGTTCGTTACCGCATGCCCCCTGACGCTGCCATTTCTGAAGCGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGc                      <  2:403655/140‑1 (MQ=255)                                                                                                                aTAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGt                 <  2:1063013/140‑1 (MQ=255)                                                                                                                   gCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGa              >  2:1489699/1‑140 (MQ=255)                                                                                                                       tcgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATc          >  2:1428326/1‑140 (MQ=255)                                                                                                                        cgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:1471906/1‑140 (MQ=255)                                                                                                                        cgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:1333124/1‑140 (MQ=255)                                                                                                                        cgttATCGCATGCCTCCTGACGCCGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:863238/1‑140 (MQ=255)                                                                                                                               gCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCaaa  <  1:833228/140‑1 (MQ=255)                                                                                                                               gCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCaaa  <  1:1471906/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAA  >  NC_000913/606925‑607190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑