Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,111	A→G	V139A (GTC→GCC)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,111	0	A	G	100.0%	317.9 / NA	84	V139A (GTC→GCC)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/38);  total (46/38)

GCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGC  >  NC_000913/4558979‑4559244                                                                                                                                     |                                                                                                                                      gccgccATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATa                                                                                                                                >  2:1497845/1‑140 (MQ=255)     ccATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAgcg                                                                                                                            <  2:428722/140‑1 (MQ=255)     ccATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAgcg                                                                                                                            >  2:622509/1‑140 (MQ=255)        tatTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCg                                                                                                                         <  2:1436596/140‑1 (MQ=255)               cAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCa                                                                                                                  >  2:37296/1‑140 (MQ=255)                  gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATcc                                                                                                               >  2:319255/1‑140 (MQ=255)                     aTAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAgg                                                                                                            >  2:1424273/1‑140 (MQ=255)                       aaaCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGgcg                                                                                                          >  1:1057208/1‑140 (MQ=255)                       aaaCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGgcg                                                                                                          >  2:817244/1‑140 (MQ=255)                       aaaCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGgcg                                                                                                          >  1:1496452/1‑140 (MQ=255)                          cGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTg                                                                                                       <  1:979652/140‑1 (MQ=255)                            tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCATGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGTTAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                     >  1:431508/1‑140 (MQ=255)                            tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                     <  1:123206/140‑1 (MQ=255)                            tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                     <  1:83033/140‑1 (MQ=255)                            tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                     <  2:1151084/140‑1 (MQ=255)                             ccGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATg                                                                                                    >  2:1112336/1‑140 (MQ=255)                             ccGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATg                                                                                                    >  2:644258/1‑140 (MQ=255)                              cGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGc                                                                                                   >  2:699356/1‑140 (MQ=255)                              cGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGc                                                                                                   >  1:524656/1‑140 (MQ=255)                                gTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct                                                                                                 <  1:639657/140‑1 (MQ=255)                                           cGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAg                                                                                      >  1:556721/1‑140 (MQ=255)                                              tGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAgcgc                                                                                   <  1:1424273/140‑1 (MQ=255)                                              tGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAgcgc                                                                                   >  1:600689/1‑140 (MQ=255)                                               gATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCa                                                                                  <  2:1274438/140‑1 (MQ=255)                                                aTCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                 <  1:929737/140‑1 (MQ=255)                                                aTCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                 >  1:48920/1‑140 (MQ=255)                                                aTCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                 <  1:635497/140‑1 (MQ=255)                                                   agagATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACggg                                                                              >  2:173963/1‑140 (MQ=255)                                                   agagATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACggg                                                                              >  2:1488321/1‑140 (MQ=255)                                                   agagATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACggg                                                                              >  2:743787/1‑140 (MQ=255)                                                      gaTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCt                                                                           >  2:1315845/1‑140 (MQ=255)                                                        tGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATGGAGCGCACGGGTCTTg                                                                         <  1:1497845/140‑1 (MQ=255)                                                         ggCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTgg                                                                        <  1:572988/140‑1 (MQ=255)                                                         ggCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTgg                                                                        >  1:27169/1‑140 (MQ=255)                                                          gcgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGc                                                                       >  1:1142759/1‑140 (MQ=255)                                                          gcgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGc                                                                       >  2:112306/1‑140 (MQ=255)                                                          gcgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGc                                                                       >  1:311399/1‑140 (MQ=255)                                                           cgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                      <  2:508367/140‑1 (MQ=255)                                                           cgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                      >  1:844779/1‑140 (MQ=255)                                                           cgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                      <  2:1467524/140‑1 (MQ=255)                                                           cgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAAGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                      >  2:1190535/1‑140 (MQ=255)                                                                    cGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGAt                                                             >  2:846908/1‑140 (MQ=255)                                                                     gCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGAtt                                                            <  2:231874/140‑1 (MQ=255)                                                                      ccAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTc                                                           <  1:319255/140‑1 (MQ=255)                                                                        aaTCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCg                                                         >  2:63149/1‑140 (MQ=255)                                                                         aTCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCTAGCAGGGATTCCgg                                                        <  2:524656/140‑1 (MQ=255)                                                                          tCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTTCggg                                                       <  2:1173680/140‑1 (MQ=255)                                                                          tCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCggg                                                       <  1:585605/140‑1 (MQ=255)                                                                          tCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCggg                                                       >  2:1287439/1‑140 (MQ=255)                                                                          tCACACGATCGATAATCGCCACGTCTTTTGCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCggg                                                       >  1:99319/1‑140 (MQ=255)                                                                            acgcgatcgatAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTg                                                     <  2:524001/137‑1 (MQ=255)                                                                            acacGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTg                                                     <  2:326445/140‑1 (MQ=255)                                                                               cgatcgatAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCg                                                  <  1:622509/140‑1 (MQ=255)                                                                                gatcgatAATCGCCCCGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCga                                                 <  1:231813/140‑1 (MQ=255)                                                                                     atAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                            >  1:280532/1‑140 (MQ=255)                                                                                     atAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                            >  2:857621/1‑140 (MQ=255)                                                                                       aaTCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATag                                          <  1:1112336/140‑1 (MQ=255)                                                                                       aaTCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATAGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATag                                          <  1:616161/140‑1 (MQ=255)                                                                                          cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                       <  1:112306/140‑1 (MQ=255)                                                                                          cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                       <  1:846908/140‑1 (MQ=255)                                                                                          cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                       <  1:386769/140‑1 (MQ=255)                                                                                            tcACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  2:1363434/2‑140 (MQ=255)                                                                                            ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  2:930091/1‑140 (MQ=255)                                                                                            ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  1:1348430/1‑140 (MQ=255)                                                                                            ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  1:1377526/1‑140 (MQ=255)                                                                                            ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  1:592667/1‑140 (MQ=255)                                                                                            ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                     >  2:489975/1‑140 (MQ=255)                                                                                              aCGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGt                                   <  2:229653/140‑1 (MQ=255)                                                                                                     tttCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCcagc                            <  2:556721/140‑1 (MQ=255)                                                                                                      ttCCACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCcagca                           >  2:404099/1‑140 (MQ=255)                                                                                                        ccccGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGCCCTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGa                         <  1:46041/137‑1 (MQ=255)                                                                                                        ccACGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGa                         <  1:37296/140‑1 (MQ=255)                                                                                                          aCGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                       <  2:797581/140‑1 (MQ=255)                                                                                                          aCGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                       <  1:775940/140‑1 (MQ=255)                                                                                                           cGGAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacca                      >  2:201782/1‑140 (MQ=255)                                                                                                             gAACCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCTGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaac                    <  1:1190535/140‑1 (MQ=255)                                                                                                              aaCCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacc                   >  2:531701/1‑140 (MQ=255)                                                                                                              aaCCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacc                   >  2:882157/1‑140 (MQ=255)                                                                                                              aaCCCGTAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacc                   >  2:479798/1‑140 (MQ=255)                                                                                                                    tAATGGTGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgac             >  1:462744/1‑140 (MQ=255)                                                                                                                          tGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgacg       <  1:228352/140‑1 (MQ=255)                                                                                                                          tGCGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgacg       >  2:702431/1‑140 (MQ=255)                                                                                                                            cGGGAAGGGGCATGTTAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGcc     <  2:431508/140‑1 (MQ=255)                                                                                                                            cGGGAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGcc     <  1:1315845/140‑1 (MQ=255)                                                                                                                               gAAGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGc  >  1:981175/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      GCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGC  >  NC_000913/4558979‑4559244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑