Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,359	C→T	S69S (AGC→AGT)	tolQ →	Tol‑Pal system protein TolQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,359	0	C	T	100.0%	285.1 / NA	84	S69S (AGC→AGT)	tolQ	Tol‑Pal system protein TolQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/42);  total (42/42)

ATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGCCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATC  >  NC_000913/775225‑775494                                                                                                                                       |                                                                                                                                        attGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAggg                                                                                                                                    >  2:1377152/1‑140 (MQ=255) attGGTTTTTCAATCGCATATTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAggg                                                                                                                                    >  2:1058749/1‑140 (MQ=255)   tGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGa                                                                                                                                  >  2:469024/1‑140 (MQ=255)   tGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGa                                                                                                                                  >  2:385722/1‑140 (MQ=255)    ggTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGCCCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGaa                                                                                                                                 <  2:499773/140‑1 (MQ=255)    ggTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGaa                                                                                                                                 <  1:683796/140‑1 (MQ=255)    ggTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGaa                                                                                                                                 <  2:513961/140‑1 (MQ=255)     gTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGaaa                                                                                                                                <  2:555629/140‑1 (MQ=255)         ttCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACggg                                                                                                                            <  1:570302/140‑1 (MQ=255)          tCAATCGTTTCTTGGACCATTATTATCCCGCGTAACCGTATTCTTAACGCGGCGGCGCGCGAAGCCGAAGAGTTGGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGa                                                                                                                           <  2:1453815/140‑1 (MQ=255)          tCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGa                                                                                                                           <  1:1029492/140‑1 (MQ=255)           cAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGAt                                                                                                                          <  1:224292/140‑1 (MQ=255)                 cATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTg                                                                                                                    >  1:330451/1‑140 (MQ=255)                 cATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTg                                                                                                                    >  2:440583/1‑140 (MQ=255)                        ggCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATAGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGtt                                                                                                             >  1:991398/1‑140 (MQ=255)                              tattatCCGGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAAc                                                                                                       <  1:476904/140‑1 (MQ=255)                              tattatCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAAc                                                                                                       <  1:305291/140‑1 (MQ=255)                                ttatCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACaa                                                                                                     <  1:625317/140‑1 (MQ=255)                                     cAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCtt                                                                                                <  2:406979/140‑1 (MQ=255)                                       gCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCtttt                                                                                              >  2:295317/1‑140 (MQ=255)                                        cGGACCTGAATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:1416340/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:1342429/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:1120743/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:58819/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:916570/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  2:99224/1‑140 (MQ=255)                                        cGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTa                                                                                             >  1:205773/1‑140 (MQ=255)                                          gACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGCAGATAAATTCTGGACTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACa                                                                                           >  2:352708/1‑140 (MQ=255)                                          gACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACa                                                                                           >  1:1243024/1‑140 (MQ=255)                                             ccGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCg                                                                                        >  2:1027550/1‑140 (MQ=255)                                             ccGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCg                                                                                        >  2:1023590/1‑140 (MQ=255)                                                tATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGt                                                                                     <  1:241806/140‑1 (MQ=255)                                                 aTTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGtt                                                                                    >  1:1356932/1‑140 (MQ=255)                                                   tCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCa                                                                                  <  1:686518/140‑1 (MQ=255)                                                   tCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCa                                                                                  <  1:125721/140‑1 (MQ=255)                                                     ttAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCaaa                                                                                >  1:48288/1‑140 (MQ=255)                                                     ttAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCaaa                                                                                >  2:370323/1‑140 (MQ=255)                                                       aaCGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAaga                                                                              >  1:296462/1‑140 (MQ=255)                                                        aCGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAagag                                                                             >  2:914617/1‑140 (MQ=255)                                                            aGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTtg                                                                         <  2:1172179/140‑1 (MQ=255)                                                            aGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTtg                                                                         >  2:116979/1‑140 (MQ=255)                                                            aGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTtg                                                                         >  2:889773/1‑140 (MQ=255)                                                             gcggcgCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTtgt                                                                        >  1:169527/1‑140 (MQ=255)                                                                 cgcgcgAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTgcgc                                                                    >  1:980269/1‑140 (MQ=255)                                                                          ccgaagcGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGt                                                           <  1:1416340/140‑1 (MQ=255)                                                                               gcGTTTAATGTTAAATTCTGGTCCGGAATCGACCTCTCACGTCTCTATCAAGAGAGTCAGGGGAACCGGGCTAATCTGCCTGGTTCGGAACAAGTCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCaa                                                      <  2:497789/140‑1 (MQ=255)                                                                                gcgttGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAAt                                                     <  1:924796/137‑1 (MQ=255)                                                                                cGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAAt                                                     >  1:1030762/1‑140 (MQ=255)                                                                                cGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAAt                                                     <  1:440583/140‑1 (MQ=255)                                                                                  tttGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAg                                                   >  2:974706/1‑140 (MQ=255)                                                                                  tttGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAg                                                   >  2:916378/1‑140 (MQ=255)                                                                                  tttGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAg                                                   >  2:1418002/1‑140 (MQ=255)                                                                                             aTTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCgg                                        >  2:1327766/1‑140 (MQ=255)                                                                                              ttCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGa                                       <  2:567518/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     >  1:1249042/1‑140 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  2:841471/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  2:516446/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  1:74839/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  2:330451/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  2:19239/140‑1 (MQ=255)                                                                                                ctggtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  1:1385428/140‑1 (MQ=255)                                                                                                ctggtctggAATCAAACTCACTCGCCTCTATCAAGAGAGTCAGGGAAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAg                                     <  2:991398/140‑1 (MQ=255)                                                                                                   gtctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCg                                  >  1:1183010/1‑140 (MQ=255)                                                                                                    tctggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGt                                 <  1:1120743/140‑1 (MQ=255)                                                                                                       ggAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGt                              <  2:1089562/140‑1 (MQ=255)                                                                                                        gAATCGAACTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTg                             >  2:611566/1‑140 (MQ=255)                                                                                                              aaCTCTCTCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAggg                       >  1:771512/1‑140 (MQ=255)                                                                                                                ctctctCGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGc                     <  1:1272709/140‑1 (MQ=255)                                                                                                                  ctctcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGt                   >  1:274174/1‑140 (MQ=255)                                                                                                                   tctcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTc                  >  1:1271519/1‑140 (MQ=255)                                                                                                                    ctcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTcg                 <  2:980269/140‑1 (MQ=255)                                                                                                                     tcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTcgc                <  1:914617/140‑1 (MQ=255)                                                                                                                     tcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTcgc                <  1:1377152/140‑1 (MQ=255)                                                                                                                     tcGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTcgc                <  2:838115/140‑1 (MQ=255)                                                                                                                      cGCCTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTcgcg               <  1:295317/140‑1 (MQ=255)                                                                                                                        ccTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTg             <  1:544871/140‑1 (MQ=255)                                                                                                                        ccTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTg             <  1:370323/140‑1 (MQ=255)                                                                                                                        ccTCTATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTg             <  2:205773/140‑1 (MQ=255)                                                                                                                          tctATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCt           <  1:1418002/140‑1 (MQ=255)                                                                                                                            tATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTAt         >  2:1477359/1‑140 (MQ=255)                                                                                                                            tATCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTAt         >  1:1043037/1‑140 (MQ=255)                                                                                                                             aTCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATg        >  2:7936/1‑140 (MQ=255)                                                                                                                             aTCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATg        <  2:296462/140‑1 (MQ=255)                                                                                                                             aTCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATg        >  1:1342854/1‑140 (MQ=255)                                                                                                                             aTCAAGAGAGTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATg        >  2:205714/1‑140 (MQ=255)                                                                                                                                 agagagTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTa    <  1:116979/140‑1 (MQ=255)                                                                                                                                 agagagTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTa    <  2:1249042/140‑1 (MQ=255)                                                                                                                                 agagagTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTa    <  2:169527/140‑1 (MQ=255)                                                                                                                                   agagTCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTAtc  >  1:667318/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCTCTCGCCTCTATCAAGAGAGCCAGGGGAAACGGGATAATCTGACTGGTTCGGAACAAATCTTTTACAGCGGGTTCAAAGAGTTTGTGCGCCTGCATCGTGCCAATAGCCATGCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATC  >  NC_000913/775225‑775494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑