Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	180,119	T→C	L295L (TTG→CTG)	dgt →	dGTP triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	180,119	0	T	C	100.0%	244.1 / NA	64	L295L (TTG→CTG)	dgt	dGTP triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

AACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACAC  >  NC_000913/179982‑180255                                                                                                                                          |                                                                                                                                         aaCTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTg                                                                                                                                        >  2:688118/1‑140 (MQ=255) aaCTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTg                                                                                                                                        >  2:57653/1‑140 (MQ=255)    ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCAc                                                                                                                                     >  2:278259/1‑140 (MQ=255)      aaTTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGa                                                                                                                                   <  2:1496648/140‑1 (MQ=255)          tGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCg                                                                                                                               <  1:148071/140‑1 (MQ=255)           ggCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGt                                                                                                                              <  1:73161/140‑1 (MQ=255)           ggCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGt                                                                                                                              >  2:1459587/1‑140 (MQ=255)              gcTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTggg                                                                                                                           <  1:972168/140‑1 (MQ=255)                tttACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGc                                                                                                                         <  1:1234751/140‑1 (MQ=255)                tttACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGc                                                                                                                         <  1:1271162/140‑1 (MQ=255)                   aCAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAg                                                                                                                      <  1:402088/140‑1 (MQ=255)                   aCAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAg                                                                                                                      <  1:221344/140‑1 (MQ=255)                     aGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCa                                                                                                                    <  1:742460/140‑1 (MQ=255)                            ttCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGaaa                                                                                                             >  2:606235/1‑140 (MQ=255)                            ttCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGaaa                                                                                                             >  2:1219072/1‑140 (MQ=255)                            ttCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGaaa                                                                                                             >  2:1075674/1‑140 (MQ=255)                                    aCGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGct                                                                                                     >  2:410719/1‑140 (MQ=255)                                           ttATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCg                                                                                              >  2:1282128/1‑140 (MQ=255)                                              tGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCtg                                                                                           >  2:658010/1‑140 (MQ=255)                                                gAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCtggt                                                                                         >  2:64604/1‑140 (MQ=255)                                                   gCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTggtggt                                                                                      >  2:280516/1‑140 (MQ=255)                                                        cgacgaCATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGaaa                                                                                 >  2:692989/1‑140 (MQ=255)                                                             acATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGcc                                                                            <  1:57653/140‑1 (MQ=255)                                                                      aTTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGaaaaa                                                                   >  2:1017907/1‑140 (MQ=255)                                                                          tgtgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCAc                                                               >  1:1320738/1‑140 (MQ=255)                                                                            tgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGc                                                             >  1:377803/1‑140 (MQ=255)                                                                            tgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGc                                                             >  2:964042/1‑140 (MQ=255)                                                                            tgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGc                                                             >  1:835221/1‑140 (MQ=255)                                                                            tgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGc                                                             >  1:135904/1‑140 (MQ=255)                                                                            tgGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGc                                                             >  1:727958/1‑140 (MQ=255)                                                                             ggCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCt                                                            >  2:412514/1‑140 (MQ=255)                                                                              gCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTc                                                           >  1:825350/1‑140 (MQ=255)                                                                                aGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCaa                                                         <  1:410719/140‑1 (MQ=255)                                                                                 gACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCaaa                                                        >  2:810036/1‑140 (MQ=255)                                                                                    cTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAg                                                     >  1:1161671/1‑140 (MQ=255)                                                                                     ttGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGt                                                    <  1:301570/140‑1 (MQ=255)                                                                                        aaGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTa                                                 <  1:1017907/140‑1 (MQ=255)                                                                                           aTGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGc                                              <  1:736516/140‑1 (MQ=255)                                                                                           aTGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGc                                              <  1:1219072/140‑1 (MQ=255)                                                                                           aTGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGc                                              <  1:563493/140‑1 (MQ=255)                                                                                           aTGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGc                                              <  1:175533/140‑1 (MQ=255)                                                                                           aTGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGc                                              <  1:278259/140‑1 (MQ=255)                                                                                            tGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGcc                                             >  1:1386083/1‑140 (MQ=255)                                                                                             gCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCg                                            <  1:1075674/140‑1 (MQ=255)                                                                                             gCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCg                                            <  1:688118/140‑1 (MQ=255)                                                                                             gCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCg                                            <  1:658010/140‑1 (MQ=255)                                                                                             gCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCg                                            <  1:444348/140‑1 (MQ=255)                                                                                              cGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGc                                           <  1:1054581/140‑1 (MQ=255)                                                                                              cGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGc                                           >  2:1267068/1‑140 (MQ=255)                                                                                               ggTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCa                                          >  1:102446/1‑140 (MQ=255)                                                                                               ggTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCa                                          >  1:312703/1‑140 (MQ=255)                                                                                                gTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAg                                         >  2:1040340/1‑140 (MQ=255)                                                                                                           aaTATTTACCGTTGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATc                              >  2:660736/1‑140 (MQ=255)                                                                                                                      ttGAGCAGCTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATg                   <  2:727958/140‑1 (MQ=255)                                                                                                                         agcagcTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTAt                >  1:123526/1‑140 (MQ=255)                                                                                                                         agcagcTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTAt                >  2:1265528/1‑140 (MQ=255)                                                                                                                            agcTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTa             <  2:312703/140‑1 (MQ=255)                                                                                                                            agcTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTa             <  1:606235/140‑1 (MQ=255)                                                                                                                              cTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGTGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACg           >  1:737364/1‑140 (MQ=255)                                                                                                                              cTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACg           >  1:1134946/1‑140 (MQ=255)                                                                                                                              cTTTATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACg           >  2:930883/1‑140 (MQ=255)                                                                                                                                ttATCATCATCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACggg         >  2:791723/1‑140 (MQ=255)                                                                                                                                   tcatcaTCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGATaa      <  1:692989/140‑1 (MQ=255)                                                                                                                                       catCTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAacac  <  1:280516/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACAC  >  NC_000913/179982‑180255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑