Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	893,579	T→C	intergenic (+146/‑205)	ybjN → / → potF	protein YbjN/putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	893,579	0	T	C	100.0%	278.0 / NA	74	intergenic (+146/‑205)	ybjN/potF	protein YbjN/putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/45);  total (29/45)

TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATAAAAAATATGC  >  NC_000913/893442‑893712                                                                                                                                          |                                                                                                                                      ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTGTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTa                                                                                                                                     >  2:1100478/1‑140 (MQ=255)  tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTaa                                                                                                                                    >  1:1452190/1‑140 (MQ=255)    gAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAAt                                                                                                                                  >  2:413185/1‑140 (MQ=255)       cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATAcc                                                                                                                               >  2:1045365/1‑140 (MQ=255)         cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGt                                                                                                                             >  1:555345/1‑140 (MQ=255)             gTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGttttt                                                                                                                         <  1:1179276/140‑1 (MQ=255)                 cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAAt                                                                                                                     <  2:963283/140‑1 (MQ=255)                 cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAAt                                                                                                                     <  1:1045365/140‑1 (MQ=255)                    gTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCg                                                                                                                  <  1:668093/140‑1 (MQ=255)                    gTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCg                                                                                                                  <  2:198949/140‑1 (MQ=255)                    gTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCg                                                                                                                  >  1:592082/1‑140 (MQ=255)                          tGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCtat                                                                                                            <  1:1413176/140‑1 (MQ=255)                              tGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATGAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGtc                                                                                                        >  1:1351337/1‑140 (MQ=255)                              tGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGtc                                                                                                        >  2:1271524/1‑140 (MQ=255)                               gcgcgcTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGtct                                                                                                       >  2:279884/1‑140 (MQ=255)                                     tGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                                                                                 <  2:762017/140‑1 (MQ=255)                                      gCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAccc                                                                                                >  1:722593/1‑140 (MQ=255)                                       cAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCt                                                                                               >  1:224819/1‑140 (MQ=255)                                         aaaTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTgg                                                                                             <  1:944856/140‑1 (MQ=255)                                          aaTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                                                                                            <  2:1091590/140‑1 (MQ=255)                                          aaTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                                                                                            <  2:321022/140‑1 (MQ=255)                                          aaTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                                                                                            <  1:168926/140‑1 (MQ=255)                                          aaTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                                                                                            <  2:164151/140‑1 (MQ=255)                                           aTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCt                                                                                           <  1:1113325/140‑1 (MQ=255)                                           aTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCt                                                                                           <  1:535277/140‑1 (MQ=255)                                           aTTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCt                                                                                           <  2:1452190/140‑1 (MQ=255)                                             tATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                                                                                         <  2:1414797/140‑1 (MQ=255)                                                         ttAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGc                                                                             >  2:1115563/1‑140 (MQ=255)                                                         ttAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGc                                                                             <  2:488117/140‑1 (MQ=255)                                                         ttAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGc                                                                             <  2:555345/140‑1 (MQ=255)                                                           aaCCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa                                                                           <  2:175189/140‑1 (MQ=255)                                                           aaCCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa                                                                           >  1:1331916/1‑140 (MQ=255)                                                           aaCCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa                                                                           <  1:1362483/140‑1 (MQ=255)                                                           aaCCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa                                                                           <  1:1496952/140‑1 (MQ=255)                                                             ccTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATg                                                                         <  2:1131048/140‑1 (MQ=255)                                                             ccTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATg                                                                         <  2:344204/140‑1 (MQ=255)                                                                tttCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtt                                                                      >  1:736815/1‑140 (MQ=255)                                                                   cTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTta                                                                   >  1:766328/1‑140 (MQ=255)                                                                       aaGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatata                                                               <  2:664870/140‑1 (MQ=255)                                                                           ttattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGa                                                           <  2:1250355/140‑1 (MQ=255)                                                                            tattTCTCTTCTCTTGTGTCGATTTGGCTTTAGCACATAGAGCTAATATGCATAAATATTTGCTAAATTCCGATTTTTATACCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAATCTTGCGATGGTTTTATATAGCGAg                                                          <  2:873891/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  1:1100478/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  2:188097/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  1:756603/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  2:710143/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  2:1327370/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  2:1447511/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAg                                                          <  1:1423098/140‑1 (MQ=255)                                                                            tattTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTACTCTTGCGATGCTTTTATATAGCGAg                                                          <  2:1464735/140‑1 (MQ=255)                                                                                         ttGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCgg                                             >  2:191520/1‑140 (MQ=255)                                                                                              tCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGaa                                        >  2:662972/1‑140 (MQ=255)                                                                                              tCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGaa                                        >  2:1165443/1‑140 (MQ=255)                                                                                                  tttGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGtt                                    <  2:722593/140‑1 (MQ=255)                                                                                                     ggCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAATCCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTtctc                                 <  2:1149346/140‑1 (MQ=255)                                                                                                     ggCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTtctc                                 <  1:966912/140‑1 (MQ=255)                                                                                                     ggCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTtctc                                 <  2:1205848/140‑1 (MQ=255)                                                                                                     ggCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTtctc                                 <  2:224819/140‑1 (MQ=255)                                                                                                      gCTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTctct                                >  2:1161058/1‑140 (MQ=255)                                                                                                       cTTTATCACATAGAGCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCtt                               <  1:530836/140‑1 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  >  2:18363/1‑140 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  >  1:1468577/1‑140 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  >  2:1079186/1‑140 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  <  2:1442124/140‑1 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  >  2:137058/1‑140 (MQ=255)                                                                                                                    agCAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACcgcg                  >  2:102983/1‑140 (MQ=255)                                                                                                                      cAAATATGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGcc                >  2:1041056/1‑140 (MQ=255)                                                                                                                         atatGCATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGAt             <  2:736815/140‑1 (MQ=255)                                                                                                                              cATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaa        >  1:26636/1‑140 (MQ=255)                                                                                                                              cATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaa        >  2:561397/1‑140 (MQ=255)                                                                                                                              cATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaa        >  2:1195750/1‑140 (MQ=255)                                                                                                                              cATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaa        >  1:1211688/1‑140 (MQ=255)                                                                                                                              cATAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaa        >  2:107585/1‑140 (MQ=255)                                                                                                                               aTAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaaa       <  1:226453/140‑1 (MQ=255)                                                                                                                               aTAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaaa       <  1:1427514/140‑1 (MQ=255)                                                                                                                               aTAAAAATTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATaaaaaa       <  2:1331916/140‑1 (MQ=255)                                                                                                                                    aaTTTGCTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATAAAAAATATGc  <  1:662972/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGCAGTGCTGGCCGGGAGAAAGTTCTCTTTTCTTACACCGCGCCGATAAAAAATATGC  >  NC_000913/893442‑893712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑