Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	995,625	A→G	F122L (TTC→CTC)	ssuD ←	FMNH2‑dependent alkanesulfonate monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	995,625	0	A	G	100.0%	214.1 / NA	58	F122L (TTC→CTC)	ssuD	FMNH2‑dependent alkanesulfonate monooxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/35);  total (23/35)

CGCCGGGAAGAGCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAACACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGAGGTTACGCTGGGACGCAGGGC  >  NC_000913/995491‑995760                                                                                                                                       |                                                                                                                                        cGCCGGGAAGAGCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTc                                                                                                                                    <  2:68990/140‑1 (MQ=255)   ccGGGAAGAGCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCg                                                                                                                                  >  2:512100/1‑140 (MQ=255)   ccGGGAAGAGCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCg                                                                                                                                  <  2:702181/140‑1 (MQ=255)            gCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGcc                                                                                                                         <  1:456865/140‑1 (MQ=255)            gCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGcc                                                                                                                         <  1:911043/140‑1 (MQ=255)              aGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAg                                                                                                                       <  1:565481/140‑1 (MQ=255)              aGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAg                                                                                                                       <  1:344575/140‑1 (MQ=255)              aGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAg                                                                                                                       <  2:1483196/140‑1 (MQ=255)              aGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAg                                                                                                                       <  1:1183617/140‑1 (MQ=255)                    gCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTtg                                                                                                                 <  2:829424/140‑1 (MQ=255)                     cTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTtgt                                                                                                                <  1:1313034/140‑1 (MQ=255)                     cTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTtgt                                                                                                                >  2:1248999/1‑140 (MQ=255)                     cTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTtgt                                                                                                                <  1:47485/140‑1 (MQ=255)                          cgcACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATc                                                                                                           >  2:1437401/1‑140 (MQ=255)                            cacaTGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGc                                                                                                         <  1:1291824/140‑1 (MQ=255)                            cacaTGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGc                                                                                                         <  1:381663/140‑1 (MQ=255)                                  aaTATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCtg                                                                                                   >  2:79375/1‑140 (MQ=255)                                     atGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGa                                                                                                <  1:47782/140‑1 (MQ=255)                                     atGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGa                                                                                                <  2:971626/140‑1 (MQ=255)                                       gTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGAcc                                                                                              <  1:297982/140‑1 (MQ=255)                                       gTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGAcc                                                                                              >  2:1316202/1‑140 (MQ=255)                                       gTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGAcc                                                                                              >  2:645062/1‑140 (MQ=255)                                       gTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGAcc                                                                                              <  1:908500/140‑1 (MQ=255)                                         ttACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAg                                                                                            >  2:1328503/1‑140 (MQ=255)                                          tACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAgg                                                                                           <  1:1414762/140‑1 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  2:1489283/1‑140 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          <  2:243789/140‑1 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  1:1025943/1‑140 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  1:1009821/1‑140 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  1:733996/1‑140 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  1:1173139/1‑140 (MQ=255)                                           aCCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGt                                                                                          >  1:75202/1‑140 (MQ=255)                                            ccGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCCCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGtt                                                                                         >  1:1416906/1‑140 (MQ=255)                                             cGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTa                                                                                        >  1:1102503/1‑140 (MQ=255)                                                tGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAaac                                                                                     <  1:1437401/140‑1 (MQ=255)                                                 gAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGCTAaaca                                                                                    >  2:25737/1‑140 (MQ=255)                                                       gACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTACCAGCTCTTGTGGATCGCTGCCTGTGACCAGGGTAAACAACGCAc                                                                              >  2:766779/1‑140 (MQ=255)                                                        aCGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACg                                                                             <  1:512100/140‑1 (MQ=255)                                                          ggTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTc                                                                           <  1:1248999/140‑1 (MQ=255)                                                                    tGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTgagag                                                                 <  1:530781/140‑1 (MQ=255)                                                                    tGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTgagag                                                                 <  1:1328503/140‑1 (MQ=255)                                                                              gccgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGc                                                       >  2:277095/1‑140 (MQ=255)                                                                               ccgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCg                                                      <  1:906693/140‑1 (MQ=255)                                                                               ccgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCAAGTCCATTTGAGAGACGGTCAAGCg                                                      <  2:1102503/140‑1 (MQ=255)                                                                                cgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGt                                                     >  1:426794/1‑140 (MQ=255)                                                                                cgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGt                                                     >  1:279145/1‑140 (MQ=255)                                                                                cgccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGt                                                     >  2:1046702/1‑140 (MQ=255)                                                                                  ccAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTgg                                                   <  2:1009821/140‑1 (MQ=255)                                                                                   cAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTggc                                                  >  2:265480/1‑140 (MQ=255)                                                                                            gTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTggcg                                         <  1:79375/140‑1 (MQ=255)                                                                                                  tCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGggcggc                                   <  1:1316202/140‑1 (MQ=255)                                                                                                     gccgAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAAc                                <  2:807530/140‑1 (MQ=255)                                                                                                      ccgAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACg                               <  2:733996/140‑1 (MQ=255)                                                                                                      ccgAGGCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACg                               <  2:1416906/140‑1 (MQ=255)                                                                                                           gCTTCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAgg                          <  2:426794/140‑1 (MQ=255)                                                                                                              tCGTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGa                       <  1:484404/140‑1 (MQ=255)                                                                                                                gTAGCGCTCGCTATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGAgg                     <  1:322085/140‑1 (MQ=255)                                                                                                                        cgctATGATCAAGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGAGGTTACGCTg             >  2:787592/1‑140 (MQ=255)                                                                                                                                   aGGAGCACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGAGGTTACGCTGGGACGCAGGGc  >  1:246943/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCCGGGAAGAGCAGTTTTGCTCCGCGCACATGAATATGTTTACCGTTGAAATCGACGGTTTCTCTCTGCAATAAACGCCGCCAGACCTGGGTAAATTCCGCCGAGGCTTCGTAGCGCTCGCTATGATCAAGGAACACTCCGTCGCCTGCCAGCTCTTGTGGATCGCTGCCTGTGACCAGGTTAAACAACGCACGTCCATTTGAGAGACGGTCAAGCGTGGCGGCCTGGCGGGCGGCAACGGTAGGTGAGGTTACGCTGGGACGCAGGGC  >  NC_000913/995491‑995760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑