Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,074,398	A→G	T53A (ACC→GCC)	rutR →	DNA‑binding transcriptional dual regulator RutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,398	0	A	G	100.0%	277.7 / NA	73	T53A (ACC→GCC)	rutR	DNA‑binding transcriptional dual regulator RutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/44);  total (29/44)

AAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACA  >  NC_000913/1074261‑1074533                                                                                                                                          |                                                                                                                                        aaaCAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGcc                                                                                                                                       >  2:1178693/1‑140 (MQ=255)  aacaacGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCa                                                                                                                                      >  1:584444/1‑140 (MQ=255)  aacaacGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCa                                                                                                                                      <  1:305981/140‑1 (MQ=255)    caacGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGTGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAAt                                                                                                                                    <  2:897335/140‑1 (MQ=255)     aacGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATc                                                                                                                                   <  2:961095/140‑1 (MQ=255)     aacGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATc                                                                                                                                   <  2:1422966/140‑1 (MQ=255)       cGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATctg                                                                                                                                 <  2:643986/140‑1 (MQ=255)       cGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATctg                                                                                                                                 <  2:55326/140‑1 (MQ=255)       cGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCATAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATctg                                                                                                                                 <  2:1482756/140‑1 (MQ=255)        gggTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCATAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATctgc                                                                                                                                >  1:1369297/1‑140 (MQ=255)        gggTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATctgc                                                                                                                                >  1:651442/1‑140 (MQ=255)                   cgcgcgCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGGGCAGATAGCAGAGCTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACttt                                                                                                                     >  2:1163492/1‑140 (MQ=255)                   cgcgcgCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACttt                                                                                                                     <  1:998861/140‑1 (MQ=255)                   cgcgcgCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACttt                                                                                                                     <  2:553043/140‑1 (MQ=255)                         cAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCa                                                                                                               >  1:16112/1‑140 (MQ=255)                          aGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCaa                                                                                                              <  1:1012050/140‑1 (MQ=255)                             aaGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAag                                                                                                           <  1:1388649/140‑1 (MQ=255)                              aGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAaga                                                                                                          <  1:1209834/140‑1 (MQ=255)                               gcgcGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAagag                                                                                                         <  2:584444/140‑1 (MQ=255)                               gcgcGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAagag                                                                                                         <  1:341293/140‑1 (MQ=255)                               gcgcGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAagag                                                                                                         <  2:1304829/140‑1 (MQ=255)                               gcgcGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAagag                                                                                                         <  1:1403819/140‑1 (MQ=255)                               gcgcGAAG‑AAAAAGCGATTCTTAGCGCAGCGCTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAgg                                                                                                        <  2:593243/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:1046937/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  2:821918/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  2:477830/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      >  2:1461888/1‑140 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  2:1329214/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      >  2:1268636/1‑140 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:1404069/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:169434/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:54976/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:375710/140‑1 (MQ=255)                                  cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                                                                                      <  1:1418426/140‑1 (MQ=255)                                    aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCt                                                                                                    <  1:859943/140‑1 (MQ=255)                                    aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCt                                                                                                    >  1:672786/1‑140 (MQ=255)                                    aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCt                                                                                                    >  1:618668/1‑140 (MQ=255)                                    aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCt                                                                                                    >  2:539208/1‑140 (MQ=255)                                        aaaaaGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGtat                                                                                                >  2:951001/1‑140 (MQ=255)                                                 ttCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGTACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGtg                                                                                       >  1:324893/1‑140 (MQ=255)                                                 ttCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGtg                                                                                       <  2:1074923/140‑1 (MQ=255)                                                                  gACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGa                                                                      >  1:566469/1‑140 (MQ=255)                                                                  gACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGa                                                                      >  1:687907/1‑140 (MQ=255)                                                                     acTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGatat                                                                   <  1:833868/140‑1 (MQ=255)                                                                       tttttCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATct                                                                 >  1:685687/1‑140 (MQ=255)                                                                       tttttCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATct                                                                 >  2:266150/1‑140 (MQ=255)                                                                       tttttCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATct                                                                 >  2:359592/1‑140 (MQ=255)                                                                                aTTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCAc                                                        <  2:1007994/140‑1 (MQ=255)                                                                                   cGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGt                                                     >  1:173312/1‑140 (MQ=255)                                                                                                acaAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCg                                        >  2:1374724/1‑140 (MQ=255)                                                                                                  aaGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTg                                      <  1:813493/140‑1 (MQ=255)                                                                                                  aaGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTg                                      <  1:821248/140‑1 (MQ=255)                                                                                                         gAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGAttt                               >  1:636932/1‑140 (MQ=255)                                                                                                         gAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGAttt                               >  1:268353/1‑140 (MQ=255)                                                                                                              gATCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCATTAAAAGCGTTTCGTGAAGATTTCGccc                          >  1:623552/1‑140 (MQ=255)                                                                                                               aTCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                         >  2:494507/1‑140 (MQ=255)                                                                                                               aTCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAACGTTTCGTGAAGATTTCGcccc                         >  2:750360/1‑140 (MQ=255)                                                                                                                tCGCAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCg                        >  2:1146346/1‑140 (MQ=255)                                                                                                                   cAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTTCTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                     <  2:266784/140‑1 (MQ=255)                                                                                                                   cAGAGTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                     >  1:678786/1‑140 (MQ=255)                                                                                                                     gagTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggc                   <  2:329302/140‑1 (MQ=255)                                                                                                                       gTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggcgg                 <  1:1304426/140‑1 (MQ=255)                                                                                                                       gTTGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggcgg                 <  2:687907/140‑1 (MQ=255)                                                                                                                        ttGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggcggc                <  2:651442/140‑1 (MQ=255)                                                                                                                        ttGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggcggc                <  2:760358/140‑1 (MQ=255)                                                                                                                        ttGGCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggcggc                <  2:106782/140‑1 (MQ=255)                                                                                                                          ggCGGGTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGa              <  1:887430/140‑1 (MQ=255)                                                                                                                             gggTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa           <  1:1178693/140‑1 (MQ=255)                                                                                                                             gggTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa           >  1:366828/1‑140 (MQ=255)                                                                                                                             gggTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa           <  1:266150/140‑1 (MQ=255)                                                                                                                             gggTGTTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa           <  2:16112/140‑1 (MQ=255)                                                                                                                               gtgtTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCaaa         <  1:1049895/140‑1 (MQ=255)                                                                                                                               gtgtTTCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCaaa         <  2:1390101/140‑1 (MQ=255)                                                                                                                                  tttCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAagag      >  1:1162524/1‑140 (MQ=255)                                                                                                                                  tttCAAAAGCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAagag      >  2:1022571/1‑140 (MQ=255)                                                                                                                                      aaaagCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACa  <  2:672786/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACA  >  NC_000913/1074261‑1074533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑