Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,140	T→C	T410A (ACT→GCT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,140	0	T	C	100.0%	244.5 / NA	67	T410A (ACT→GCT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

TTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCT  >  NC_000913/1143004‑1143273                                                                                                                                          |                                                                                                                                      ttttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCAcc                                                                                                                                    >  1:1300081/1‑140 (MQ=255)     cattcaGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGaa                                                                                                                                <  1:46022/140‑1 (MQ=255)              ggTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGa                                                                                                                       <  2:962938/140‑1 (MQ=255)                 aagaagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACaa                                                                                                                    >  2:376940/1‑140 (MQ=255)                     agCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGACACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGt                                                                                                                <  2:1460386/140‑1 (MQ=255)                         aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                            >  1:763800/1‑140 (MQ=255)                         aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                            >  2:748870/1‑140 (MQ=255)                         aTTGGCACAGGAACAATAGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCCACCGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                            >  2:185600/1‑140 (MQ=255)                          ttGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatga                                                                                                           >  2:1471083/1‑140 (MQ=255)                           tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                          <  1:926788/140‑1 (MQ=255)                           tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                          <  1:237819/140‑1 (MQ=255)                               acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGa                                                                                                      <  1:803292/140‑1 (MQ=255)                               acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGa                                                                                                      <  2:470959/140‑1 (MQ=255)                                caGGAACAATGGCGTGAACTTTCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                     <  2:1266296/140‑1 (MQ=255)                                caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                     <  2:1164313/140‑1 (MQ=255)                                caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                     <  1:1433907/140‑1 (MQ=255)                                     aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                <  2:1491493/140‑1 (MQ=255)                                     aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                <  1:387919/140‑1 (MQ=255)                                        aTGGCGTGAACTTCCTGAGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAg                                                                                             >  1:1458242/1‑140 (MQ=255)                                           gCGTGAACTTCCTGGGCGTTCTCTTTCAGCGATTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGa                                                                                          >  1:1383680/1‑140 (MQ=255)                                              tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATgg                                                                                       >  1:279016/1‑140 (MQ=255)                                                 aCTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCCGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCt                                                                                    >  2:690340/1‑140 (MQ=255)                                                     ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                                                                                >  1:243314/1‑140 (MQ=255)                                                           tgtTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGa                                                                          >  1:415595/1‑140 (MQ=255)                                                           tgtTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGa                                                                          >  1:13287/1‑140 (MQ=255)                                                            gtTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc                                                                         <  1:379353/140‑1 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                      >  1:1051146/1‑140 (MQ=255)                                                               ctctTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                      <  1:748870/140‑1 (MQ=255)                                                                 ctTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc                                                                    <  1:1268074/140‑1 (MQ=255)                                                                    tCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt                                                                 <  1:6007/140‑1 (MQ=255)                                                                    tCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt                                                                 <  2:792425/140‑1 (MQ=255)                                                                    tCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt                                                                 <  1:206939/140‑1 (MQ=255)                                                                    tCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt                                                                 >  1:38119/1‑140 (MQ=255)                                                                     cAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCATCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAttt                                                                >  2:1410921/1‑140 (MQ=255)                                                                       gcgcTTCTTCTTCGAATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc                                                               <  2:1210946/140‑1 (MQ=255)                                                                       gcgcTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc                                                              >  2:1190231/1‑140 (MQ=255)                                                                       gcgcTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc                                                              >  2:998014/1‑140 (MQ=255)                                                                         gcTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag                                                            >  1:318301/1‑140 (MQ=255)                                                                               tcttcG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGcc                                                      <  2:279016/140‑1 (MQ=255)                                                                                 ttcG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCaa                                                    <  2:763800/140‑1 (MQ=255)                                                                                      aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCg                                                <  2:1458242/140‑1 (MQ=255)                                                                                      aTCAGACGCAGAATAGAGAGCGACAGCGATTAGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCg                                                >  1:811370/1‑140 (MQ=255)                                                                                        cAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCgag                                              >  1:1309063/1‑140 (MQ=255)                                                                                                gAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                      >  1:1322850/1‑140 (MQ=255)                                                                                                 aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                     >  2:1332258/1‑140 (MQ=255)                                                                                                 aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                     <  2:1383680/140‑1 (MQ=255)                                                                                                 aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                     >  2:475343/1‑140 (MQ=255)                                                                                                      agagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAtt                                >  2:1457984/1‑140 (MQ=255)                                                                                                      agagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAtt                                >  1:190526/1‑140 (MQ=255)                                                                                                         gCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGa                             >  1:945558/1‑140 (MQ=255)                                                                                                           gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCTGGCCAAAGCGAGAAATATGGCTGATTTGAAt                           >  1:164039/1‑140 (MQ=255)                                                                                                           gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                           >  2:502655/1‑140 (MQ=255)                                                                                                           gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                           >  2:708290/1‑140 (MQ=255)                                                                                                           gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                           >  1:1024187/1‑140 (MQ=255)                                                                                                            aCAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATa                          <  1:1471083/140‑1 (MQ=255)                                                                                                                cGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                      >  1:769952/1‑140 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcg                        >  1:850166/1‑136 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcg                        <  2:850166/136‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  1:185600/140‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  2:318301/140‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  1:376940/140‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  1:519258/140‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  1:792851/140‑1 (MQ=255)                                                                                                                  aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                    <  2:100922/140‑1 (MQ=255)                                                                                                                     cGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACgg                 <  2:970389/140‑1 (MQ=255)                                                                                                                             cgcacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACg         >  2:891595/1‑140 (MQ=255)                                                                                                                               cacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCa       <  2:38119/140‑1 (MQ=255)                                                                                                                                    tGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCt  >  1:867730/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCG‑ATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCT  >  NC_000913/1143004‑1143273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑