Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,163,379	A→G	E40E (GAA→GAG)	thiK →	thiamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,163,379	0	A	G	100.0%	172.8 / NA	46	E40E (GAA→GAG)	thiK	thiamine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/46);  total (0/46)

TATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCG  >  NC_000913/1163243‑1163510                                                                                                                                         |                                                                                                                                    tATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCAt                                                                                                                                  <  2:993679/140‑1 (MQ=255)       gTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGc                                                                                                                            <  1:1061641/140‑1 (MQ=255)         cAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCa                                                                                                                          <  2:8079/140‑1 (MQ=255)              aaaGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGtt                                                                                                                     <  1:779137/140‑1 (MQ=255)               aaGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGGTTTCTCATTGTGCATCAGGGCCAGCGctt                                                                                                                    >  1:385021/1‑140 (MQ=255)                  gTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTttgt                                                                                                                 <  1:1261091/140‑1 (MQ=255)                      cGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCCTTTTGCCTCAGGGCCCGCGGTTTGTtttg                                                                                                             >  2:413585/1‑140 (MQ=255)                      cGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCCTTTAGCATCAGGGGCAGCGTTTTGTtgtg                                                                                                             >  1:164322/1‑140 (MQ=255)                      cGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGGTTTTTCCTTTTGCATCAAGGGCCGCGTTTttttgtt                                                                                                             >  1:1366425/1‑139 (MQ=255)                        tttCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTGGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGACAGCGTTTTGTTGTGCg                                                                                                           <  2:315963/140‑1 (MQ=255)                         ttCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGt                                                                                                          <  1:229599/140‑1 (MQ=255)                          tCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTc                                                                                                         <  2:573929/140‑1 (MQ=255)                           cGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCCTTTTGCATCAGGGGCCGCGGTTTTTtttgtggcg                                                                                                        >  2:560386/1‑135 (MQ=255)                            gcagcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAg                                                                                                       <  1:1251181/140‑1 (MQ=255)                            gcagcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAg                                                                                                       <  1:233512/140‑1 (MQ=255)                             cagcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCTTTTTGGATCAAGGCCACCGCTTTTTTGTTCGTCGGc                                                                                                      >  1:916412/1‑140 (MQ=255)                              agcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGcc                                                                                                     <  2:722987/140‑1 (MQ=255)                                 aataatCCCGTCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGATTTCTCATTGTGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  2:1127689/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  2:616553/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  2:170023/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  2:1272040/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  1:1008878/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  1:457892/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  1:17743/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  1:313483/140‑1 (MQ=255)                                 aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCa                                                                                                  <  1:504896/140‑1 (MQ=255)                                   taatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGGTTTTTTTTTTTGCAACAGGGGCAGCGGTTTTTTTTTGGTCGGCCGcccc                                                                                                >  1:1134789/1‑137 (MQ=255)                                           tCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGAt                                                                                        <  1:681435/140‑1 (MQ=255)                                                 gcgACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGcgccg                                                                                  <  1:552784/140‑1 (MQ=255)                                                 gcgACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGcgccg                                                                                  <  1:1295708/140‑1 (MQ=255)                                                  cgacgaATTGCTGTCGCGCTTTTTCCCGCAGTATCGTCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGcgccgc                                                                                 <  2:640171/140‑1 (MQ=255)                                                         ttGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCcgc                                                                          <  1:628689/140‑1 (MQ=255)                                                              gTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCTTTTTGCATCAGGGCCCACGGTTTGTTGTGGGTCAGCCCCACCAGCCTGATGCGCCCCAGGCCCCGTTCt                                                                     >  2:928057/1‑140 (MQ=255)                                                              gTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCt                                                                     <  2:951924/140‑1 (MQ=255)                                                                           ccGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCg                                                        <  1:1302887/140‑1 (MQ=255)                                                                              cAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGTAGTCCGCGTTCTTGCGCCAGTATCGGGc                                                     <  2:203559/140‑1 (MQ=255)                                                                              cAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGc                                                     <  2:939328/140‑1 (MQ=255)                                                                               aGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCt                                                    <  1:470762/140‑1 (MQ=255)                                                                                      tcCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATcac                                             <  2:164322/140‑1 (MQ=255)                                                                                         cGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAAc                                          <  1:448064/140‑1 (MQ=255)                                                                                           tCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTa                                        <  2:1366425/140‑1 (MQ=255)                                                                                           tCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTa                                        <  2:362009/140‑1 (MQ=255)                                                                                                acgTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgc                                   <  2:250023/140‑1 (MQ=255)                                                                                                acgTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgc                                   <  1:198024/140‑1 (MQ=255)                                                                                                acgTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgc                                   <  1:524033/140‑1 (MQ=255)                                                                                                acgTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCACACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgc                                   <  1:928057/140‑1 (MQ=255)                                                                                                          gTGGGCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCa                         <  1:256188/140‑1 (MQ=255)                                                                                                             ggCTTAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCg                      <  2:766530/140‑1 (MQ=255)                                                                                                                ttAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAg                   <  1:1199255/140‑1 (MQ=255)                                                                                                                 tAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGc                  <  1:953539/140‑1 (MQ=255)                                                                                                                 tAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGc                  <  2:1134789/140‑1 (MQ=255)                                                                                                                 tAGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCCTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGc                  <  2:782690/140‑1 (MQ=255)                                                                                                                  aGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGcc                 <  1:50724/140‑1 (MQ=255)                                                                                                                  aGTGGCGGGAGTTTTCTCATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCACACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGcc                 <  1:1155622/140‑1 (MQ=255)                                                                                                                                 ctcATTGAGCATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCg  <  1:1441321/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCG  >  NC_000913/1163243‑1163510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑