| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,329,979 | C→T | S216S (AGC→AGT) | sohB → | S49 peptidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,329,979 | 0 | C | T | 98.0% | 160.3 / NA | 50 | S216S (AGC→AGT) | sohB | S49 peptidase family protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/23); minor base G (1/0); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGGCGGTTACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGCAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAGTTGTTTAAAG > NC_000913/1329863‑1330109 | aGCGGCGGTTACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGca < 1:255831/140‑1 (MQ=255) ggTTACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGc < 1:1230758/140‑1 (MQ=255) ttACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCg < 1:885280/140‑1 (MQ=255) ttACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCg < 2:717339/140‑1 (MQ=255) ttACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCg < 1:734606/140‑1 (MQ=255) tACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCgg < 2:1392329/140‑1 (MQ=255) aCATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCggg < 1:508341/140‑1 (MQ=255) aCATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCggg < 2:147969/140‑1 (MQ=255) ggCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGtata > 1:1283851/1‑140 (MQ=255) gCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATaa > 1:1391013/1‑140 (MQ=255) tgtgtgGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAgcg < 1:709008/140‑1 (MQ=255) tgtgtgGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAgcg < 1:435603/140‑1 (MQ=255) tgtgtgGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAgcg < 2:410544/140‑1 (MQ=255) gtgGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCAc < 1:1197765/140‑1 (MQ=255) gtgGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCAc < 1:500624/140‑1 (MQ=255) cGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTg > 2:1369138/1‑140 (MQ=255) cGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTg > 1:523951/1‑140 (MQ=255) cGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACCGCCGGGCAGTATAAGCGCACGcag > 1:288017/1‑138 (MQ=255) cGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTACACACTGCCGGGCAGTATAAGCGCACGCTg > 1:1472862/1‑140 (MQ=255) cGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGGAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTg > 1:314925/1‑140 (MQ=255) aaaaTTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGtt > 2:566432/1‑140 (MQ=255) ccGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGaa < 2:704457/140‑1 (MQ=255) tttGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCga > 1:260023/1‑140 (MQ=255) tttGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCga > 1:124379/1‑140 (MQ=255) tgtgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGc > 2:286599/1‑140 (MQ=255) tgtgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGc > 1:1147885/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 2:98052/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 2:583672/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 2:899266/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 1:1178218/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 1:1356562/1‑140 (MQ=255) tgGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCgg > 1:261894/1‑140 (MQ=255) ggTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGga > 1:1098517/1‑140 (MQ=255) ggTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGga > 1:1360245/1‑140 (MQ=255) ggTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGga > 2:332506/1‑140 (MQ=255) aTTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAAtt < 1:925031/140‑1 (MQ=255) aTTGGCGTGGTGGCGCAAATGACCAACTTTAACCGCTTCCTGAAAAGTAAAGGTATTGATAACGAACTGCACACCGCCGGGCAGGATAAGCGCACGCTGACGATGCTGGGCGAAAATACCGAAGAAAGGCGGGAGAAAtt > 2:1442437/1‑140 (MQ=255) gCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCcgc > 2:916371/1‑140 (MQ=255) gtggCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAaga > 2:1270680/1‑140 (MQ=255) gtggCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAaga > 1:801000/1‑140 (MQ=255) tggCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAagag < 1:534478/140‑1 (MQ=255) aTGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGa < 1:419163/140‑1 (MQ=255) aTGCCCAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGa < 2:953104/140‑1 (MQ=255) ccAACTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACg < 1:286599/140‑1 (MQ=255) aCTTTAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCAt < 1:899266/140‑1 (MQ=255) ttAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAg < 2:268794/140‑1 (MQ=255) tAACCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAgt < 2:275156/140‑1 (MQ=255) aCCGCTTCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAgttg > 2:201398/1‑140 (MQ=255) tCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAGTTGTTTaaa > 2:337524/1‑140 (MQ=255) tCCTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAGTTGTTTaaa > 2:1001408/1‑140 (MQ=255) ccTGAAAAGTAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAGTTGTTTAAAg < 2:405247/140‑1 (MQ=255) | AGCGGCGGTTACATGATGGCCTGTGTGGCGGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGCGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCCTGAAAAGCAAAGATATTGATATCGAACTGCACACTGCCGGGCAGTATAAGCGCACGCTGACGTTGCTGGGTGAAAATACCGAAGAAGGGCGGGAGAAATTCCGCGAAGAGTTGAACGAAACGCATCAGTTGTTTAAAG > NC_000913/1329863‑1330109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |